JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Analysis of Cyclic Adenosine Monophosphate (cAMP) Separation via RP-HPLC (reversed-phase high-performance liquid chromatography) by the Moment Method and the van Deemter Equation
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Korean Chemical Engineering Research
  • Volume 53, Issue 6,  2015, pp.723-729
  • Publisher : The Korean Institute of Chemical Engineers
  • DOI : 10.9713/kcer.2015.53.6.723
 Title & Authors
Analysis of Cyclic Adenosine Monophosphate (cAMP) Separation via RP-HPLC (reversed-phase high-performance liquid chromatography) by the Moment Method and the van Deemter Equation
Lee, Il Song; Ko, Kwan Young; Kim, In Ho;
  PDF(new window)
 Abstract
The moment analysis of cyclic adenosine monophosphate (cAMP) was performed using chromatograms that were obtained with the pulse input method from an octadecyl silica (ODS) high-performance liquid chromatography (HPLC) column. The general rate (GR) model was employed to calculate the first absolute moment and the second central moment. Three important coefficients for moment analysis, which are molecular diffusivity (), external mass transfer coefficient (), and intra-particle diffusivity (), were estimated by the Wilke-Chang equation, Wilson-Geankoplis equation, and comparing van Deemter equation to theoretical plate number equation, respectively. Experiments were conducted by various conditions of flow rates, methanol volume ratio of the mobile phase, and solute concentration. After the moment analysis, results were organized by van Deemter plots. Also van Deemter coefficients were compared each other to effect , , and on height equivalent to a theoretical plate (HETP, ). The value of intraparticle diffusion () was the primary factor which makes for HETP whereas external mass transfer () was disregardable factor.
 Keywords
HPLC;Parameter Estimation;Moment Analysis;Van Deemter Equation;General Rate Model;
 Language
Korean
 Cited by
 References
1.
Miyabe, K. and Suzuki, M., "Chromatography of Liquid-phase Adsorption on Octadecylsilyl-silica Gel," AIChE J., 38, 901-910 (1992). crossref(new window)

2.
Miyabe, K. and Guiochon, G., "Measurement of the Parameters of the Mass Transfer Kinetics in High Performance Liquid Chromatography," J. Sep. Sci., 26, 155-173(2003). crossref(new window)

3.
Miyabe, K., "Moment Analysis of Chromatograpic Behavior in Reversed-phase Liquid Chromatography," J. Sep. Sci., 32, 757-770(2009). crossref(new window)

4.
Choi, D. Y. and Row, K. H., "Estimation of Kinetic and Rate Parameters of Peptides by Moment Analysis," J. Ind. Eng. Chem., 10, 1052-1057(2004).

5.
Wilson, E. J. and Geankoplis, C. J., "Liquid Mass Transfer at Very Low Reynolds Numbers in Packed Beds," Ind. Eng. Chem. Fundamen, 5, 9-14(1966). crossref(new window)

6.
Grushka, E., Snyder, L. R. and Knox, J. H., "Advances in Band Spreading Theories," J. Chromatogr. Sci., 13, 25-37(1975). crossref(new window)

7.
Seirafi, H. A. and Smith, J. M., "Mass Transfer and Adsorption in Liquid Full and Trickle Beds," AIChE J., 26, 711-717(1980). crossref(new window)

8.
Gunn, D. J., "Axial and Radial Dispersion in Fixed Beds," Chem. Eng. Sci., 42, 363-373(1987). crossref(new window)

9.
Tallarek, U., Albert, K., Bayer, E. and Guiochon, G., "Measurement of Transverse and Axial Apparent Dispersion Coefficient in Packed Beds," AIChE J., 42, 3041-3054(1996). crossref(new window)

10.
Tallarek, U., Bayer, E. and Guiochon, G., "Study of Dispersion in Packed Chromatographic Columns Field Gradient Nuclear Magnetic Resonance," J. Am. Chem. Soc., 120, 1494-1505(1998). crossref(new window)

11.
Tallarek, U., Bayer, E., Dusschoten, D. V., Scheenen, T, As, V. H., Guiochon, G. and Neue, U. D., "Dynamic NMR Microscopy of Chromatographic Columns," AIChE J., 44, 1962-1975(1998). crossref(new window)

12.
Tallarek, U., Dusschoten, D. V., As, V. H., Guiochon, G. and Bayer, E., "Mass Transfer in Chromatographic Columns Studied by PFG NMR," Magn. Reson., 16, 699-702(1998). crossref(new window)

13.
Tallarek, U., Dusschoten, D. V., As, H. V., Bayer, E. and Guiochon, G., "Study of Transport Phenomena in Chromatograpic Columns by Pulsed Field Gradient NMR," J. Phys. Chem. B., 102, 3489-3497(1998).

14.
Sajonz, P., Guan-Sajonz, H., Zhong, G. and Guiochon, G., "Application of the Shock Layer Theory to the Determination of the Mass Transfer Rate Coefficient and its Concentration Dependence for Proteins on Anion Exchange Columns," Biotechnol. Prog., 13, 170-178(1997). crossref(new window)

15.
Sajonz, P., Kele, M., Zhong, G., Sellergen, B. and Guiochon, G., "Study of the Thermodynamics and Mass Transfer Kinetics of Two Enantiomers on a Polymeric Imprinted Stationary Phase," J. Chromatogr. A, 810, 1-17(1998). crossref(new window)

16.
Miyabe, K. and Guiochon, G., "Kinetic Study of the Concentraion Dependence of the Mass Transfer Rate Coefficient in Anionexchange Chromatography of Bovine Serum Albumin," Biotechnol. Prog., 15, 740-752(1999). crossref(new window)

17.
Miyabe, K., Canazzini, A., Gritti, F., Kele, M. and Guiochon, G., "Moment Analysis of Mass Transfer Kinetics in Silica Monolithic Columns," Anal. Chem., 75, 6975-6986(2003). crossref(new window)

18.
Miyabe, K. and Guiochon, G., "Surface Diffusion in Reversed-phase Liquid Chromatography," J. Chromatogr. A., 1217, 1713-1734(2010). crossref(new window)

19.
Gritti, F. and Guiochon, G., "Importance of Sample Intraparticle Diffusivity in Investigations of the Mass Transfer Mechanism in Liquid Chromatography," AIChE J., 57, 346-358(2011). crossref(new window)

20.
Silva Jr, I. J. D., Veredas, D., Carpes, M. J. S. and Santana, C. C., "Chromatographic Separation of Bupivacaine Enantiomers by HPLC: Parameter Estimation of Equilibrium and Mass Transfer Under Linear Conditions," Adsorption, 11, 123-129(2005). crossref(new window)

21.
Spoto, G., Whitehead, E., Ferraro, A., Di, Terlizzi PM., Turano, C. and Riva, F., "A Reverse-phase HPLC Method for cAMP Phosphodiesterase Activity," Anal. Biochem., 196, 207-210(1991). crossref(new window)

22.
Zhang, Y., Lu, P., Wang, H., Zhang, J., Li, H. and Liu, J., "Simultaneous HPLC Determination of cAMP and cGMP in Commercial Jujube Juice Concentrate," Food Science, 30, 321-322(2009).

23.
Miyabe, K. and Isogai, R., "Estimation of Molecular Diffusivity in Liquid Phase Systems by the Wilke-Chang Equation," J. Chromatogr. A., 1218, 6639-6645(2011). crossref(new window)

24.
Choi, D. Y. and Row, K. H., "Estimation of Kinetic and Rate Parameters of Peptides by Moment Analysis," J. Ind. Eng. Chem., 10, 1052-1057(2004).

25.
Won, H. J. and Kim, I. H., "Prediction of Formic Acid Chromatogram in Gradient Elution Chromatography," Biotechnol. Bioprocess Eng., 6, 31-36(2001). crossref(new window)

26.
Kim, J. T. and Kim, I. H., "Pulse Response Test in Medium Pressure Protein Chromatography," Korean J. Chem. Eng., 15, 575- 579(1998). crossref(new window)

27.
Wang, Y. J. and Gerard, C., "The Use of Moment Analysis in Inverse Gas Chromatography," Macromolecules, 22, 3781-3788(1989). crossref(new window)

28.
Lee, C. H., Kang, J. H. and Row, K. H., "Parameter Estimation of Perillyl Alcohol in RP-HPLC by Moment Analysis," Biotechnol. Bioprocess Eng., 7, 16-20(2002). crossref(new window)

29.
Ra, Y. J., Jung, Y. A. and Row, K. H., "Factors Affecting HETP in Capillary Gas Chromatography," Korean J. Biotechnol. Bioeng., 17, 88-92(2002).

30.
Park, I. S. and Ahn, D. Y., "Estimation of Mass Transfer Parameters of Large-pore Particles by Chromatography," Korean Chem. Eng. Res., 27, 331-338(1989).