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Liquid-Liquid Extraction for Recovery of Paclitaxel from Plant Cell Cultures by Adding Inorganic Salts
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  • Journal title : Korean Chemical Engineering Research
  • Volume 54, Issue 1,  2016, pp.135-139
  • Publisher : The Korean Institute of Chemical Engineers
  • DOI : 10.9713/kcer.2016.54.1.135
 Title & Authors
Liquid-Liquid Extraction for Recovery of Paclitaxel from Plant Cell Cultures by Adding Inorganic Salts
Ha, Geon-Soo; Kim, Jin-Hyun;
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We developed a liquid-liquid extraction method using an inorganic salt to dramatically improve the recovery efficiency of the anticancer agent paclitaxel from plant cell cultures. As a result of liquid-liquid extraction using a diverse types of inorganic salt (NaCl, KCl, , , ), NaCl gave the highest yield (~96%) and lowest partition coefficient (0.053) of paclitaxel. The optimal NaCl/solvent ratio, methylene chloride/MeOH ratio, and pure paclitaxel content for liquid-liquid extraction using NaCl were 1% (w/v), 26% (v/v), and 0.066% (w/v), respectively. Under the optimal conditions developed in the present method, most of the paclitaxel (~96%) was recovered from biomass by a single extraction step. In addition, this method facilitated 3-fold higher recovery efficiency of paclitaxel in a shorter extraction number than the conventional liquid-liquid extraction method.
Paclitaxel;Recovery;Liquid-Liquid Extraction;Inorganic Salt;NaCl;
 Cited by
식물세포배양으로부터 파클리탁셀 회수를 위한 초음파를 이용한 액-액 추출,하건수;김진현;

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