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Simple Fabrication of Adipocyte Cell Chip Using Micropatterning
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  • Journal title : Korean Chemical Engineering Research
  • Volume 54, Issue 2,  2016, pp.223-228
  • Publisher : The Korean Institute of Chemical Engineers
  • DOI : 10.9713/kcer.2016.54.2.223
 Title & Authors
Simple Fabrication of Adipocyte Cell Chip Using Micropatterning
Kim, Gi Yong; Jeong, Heon-Ho; Lee, Chang-Soo; Roh, Changhyun;
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In this study, we described a simple and facile method to generate uniform microwells poly(dimethyl siloxane) (PDMS) microstamps through micro-molding for efficient, rapid and reliable cell patterning of adipocyte differentiation. In contrast to the conventional methods, the microstamp technologies are low expensive, non-toxic, and using a small amount of solution. Recently, Orlistat known as tetrahydrolipstatin is a prescription drug designed to treat obesity which is used to aid in weight loss and help to reduce overweight obesity. Here, 3T3-L1 cells were treated under various concentration manners of Orlistat . and it was confirmed maximum 26.5% inhibition activity compared to control. Thus, we elucidated this platform can be used for the real-time analyzing of cell proliferation, adipocyte differentiation for evaluation of anti-obesity agents on cell chip. Furthermore, we except that this platform technology designed here might be readily be expanded to discover a wider variety of anti-obesity agents.
Micropatterning;Anti-obesity agents;Screening;Orlistat;
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