JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Ultrasound-Assisted Liquid-Liquid Extraction for Recovery of Paclitaxel from Plant Cell Cultures
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Korean Chemical Engineering Research
  • Volume 54, Issue 2,  2016, pp.229-233
  • Publisher : The Korean Institute of Chemical Engineers
  • DOI : 10.9713/kcer.2016.54.2.229
 Title & Authors
Ultrasound-Assisted Liquid-Liquid Extraction for Recovery of Paclitaxel from Plant Cell Cultures
Ha, Geon-Soo; Kim, Jin-Hyun;
  PDF(new window)
 Abstract
In this study, an efficient ultrasound-assisted liquid-liquid extraction process was developed for recovering of paclitaxel from plant cell cultures. The optimal ultrasonic power and operating time were 250 W and 15 min at fixed ratio of bottom phase, methylene chloride to top phase, MeOH (25%, v/v). Under the optimal conditions developed in the present method, most of the paclitaxel (~92%) was recovered from crude extract by a single extraction step. Due to the synergistic effect of ultrasound by the addition of inorganic salt, an appropriate inorganic salt concentration and the ultrasonic power were found to be required for the effective recovery of paclitaxel using ultrasound-assisted liquid-liquid extraction.
 Keywords
Paclitaxel;Recovery;Ultrasound-Assisted Liquid-Liquid Extraction;NaCl;
 Language
Korean
 Cited by
 References
1.
Kim, J. H., "Paclitaxel : Recovery and Purification in Commercialization Step," Korean J. Biotechnol. Bioeng., 21, 1-10(2006).

2.
Kim, G. J. and Kim, J. H., "Enhancement of Extraction Efficiency of Paclitaxel From Biomass Using Ionic Liquid-methanol Cosolvents Under Acidic Conditions," Process Biochem., 50, 989- 996(2015). crossref(new window)

3.
Hsiao, J. R., Leu, S. F. and Huang, B. M., "Apoptotic Mechanism of Paclitaxel-induced Cell Death in Human Head and Neck Tumor Cell Lines," J. Oral Pathol. Med., 38, 188-197(2009). crossref(new window)

4.
Rao, K., Hanuman, J., Alvarez, C., Stoy, M., Juchum, J., Davies, R. and Baxley, R., "A New Large-scale Process for Taxol and Related Taxanes from Taxus brevifolia," Pharm. Res., 12, 1003-1010(1995). crossref(new window)

5.
Choi, H. K., Son, J. S., Na, G. H., Hong, S. S., Park, Y. S. and Song, J. Y., "Mass Production of Paclitaxel by Plant Cell Culture," J. Plant Biotechnol., 29, 59-62(2002). crossref(new window)

6.
Baloglu, E. and Kingston, D. G. I., "A New Semisynthesis of Paclitaxel from Baccatin III," J. Nat. Prod., 62, 1068-1071(1999). crossref(new window)

7.
Lee, C. G. and Kim, J. H., "Optimization of Adsorbent Treatment Process for the Purification of Paclitaxel from Plat Cell Cultures of Taxus chinensis," Korean Chem. Eng. Res., 52, 497-502(2014). crossref(new window)

8.
Kim, J. H., Lim, C. B., Kang, I. S., Hong, S. S. and Lee, H. S., "The Use of a Decanter for Harvesting Biomass from Plant Cell Cultures," Korean J. Biotechnol. Bioeng., 15, 337-341(2000).

9.
Kim, G. J. and Kim, J. H., "A Simultaneous Microwave-assisted Extraction and Adsorbent Treatment Process Under Acidic Conditions for Recovery and Separation of Paclitaxel from Plant Cell," Korean J. Chem. Eng., 32, 1023-1028(2015). crossref(new window)

10.
Pyo, S. H., Song, B. K., Ju, C. H., Han, B. H. and Choi, H. J., "Effects of Adsorbent Treatment on the Purification of Paclitaxel From Cell Cultures of Taxus chinensis and Yew Tree," Process Biochem., 40, 1113-1117(2005). crossref(new window)

11.
Kim, J. H., Kang, I. S., Choi, H. K., Hong, S. S. and Lee, H. S., "A Novel Prepurification for Paclitaxel from Plant Cell Cultures," Process Biochem., 37, 679-682(2002). crossref(new window)

12.
Kim, J. H. and Hong, S. S., "Optimization of Extraction Process for Mass Production of Paclitaxel from Plant Cell Cultures," Korean J. Biotechnol. Bioeng., 15, 346-351(2000).

13.
Hyun, J. E. and Kim, J. H., "Microwave-assisted Extraction of Paclitaxel from Plant Cell Cultures," Korean J. Biotechnol. Bioeng., 23, 281-284(2008).

14.
Pyo, S. H., Park, H. B., Song, B. K., Han, B. H. and Kim, J. H., "A Large-scale Purification of Paclitaxel from Cell Cultures of Taxus chinensis," Process Biochem., 39, 1985-1991(2004). crossref(new window)

15.
Kim, J. H., "Optimization of Liquid-liquid Extraction Conditions for Paclitaxel Separation from Plant Cell Cultures," KSBB J., 24, 212-215(2009).

16.
Pachaiyappan, A., Varathan, S. and Venugopal, R., "Sonication Effect on the Reaction of 4-bromo-1-methylbenzene with Sodium Sulfide in Liquid-liquid Multi-site Phase-transfer Catalysis Condition - Kinetic Study," Ultrason. Sonochem., 23, 156-164(2015). crossref(new window)

17.
Guo, Y. X., Han, J., Zhang, D. Y., Wang, L. H. and Zhou, L. L., "An Ammonium Sulfate/ethanol Aqueous Two-phase System Combined with Ultrasonication for the Separation and Purification of Lithospermic Acid B from Salvia miltiorrhiza Bunge," Ultrason. Sonochem., 19, 719-724(2012). crossref(new window)

18.
Reza, R., Rouhollah, H. and Ahmad, L., "Ultrasound and Salt-assisted Liquid-liquid Extraction as an Efficient Method for Natural Product Extraction," Anal. Methods., 7, 3253(2015). crossref(new window)

19.
Ming, G., Huili, W., Meiping, M., Yuna, Z., Xiaohan, Y., Randy D., Dongli, D. and Xuedong, W., "Optimization of a Phase Separation Based Magnetic-stirring Salt-induced Liquid-liquid Microextraction Method for Determination of Fluoroquinolones in Food," Food Chem., 175, 181-188(2015). crossref(new window)

20.
Choi, H. K., Adams, T. L., Stahlhut, R. W., Kim, S. I., Yun, J. H., Song, B. K., Kim, J. H., Song, J. S., Hong, S. S. and Lee, H. S., "Method for Mass Production of Taxol by Semi-continuous Culture with Taxus chinensis Cell Culture," US. Patent No. 5,871,979(1999).

21.
Findik, S. and Gunduz, G., "Sonolytic Degradation of Acetic Acid in Aqueous Solutions," Ultrason. Sonochem., 14, 157-162(2007). crossref(new window)

22.
Mukesh, G., Hu, H., Arun, S. M. and Madhumita, B, R., "Sonochemical Decomposition of Volatile and Non-volatile Organic Compounds a Comparative Study," Water Res., 38, 4247-4261(2004). crossref(new window)

23.
Jagannathan, T. K., Nagarajan, R. and Ramamurthi, K., "Effect of Ultrasound on Bubble Breakup Within the Mixing Chamber of An Effervescent Atomizer," Chem. Eng. Process., 50, 305-315 (2011). crossref(new window)

24.
Ha, G. S. and Kim, J. H., "Liquid-liquid Extraction for Recovery of Paclitaxel from Plant Cell Cultures by Adding Inorganic Salts," Korean Chem. Eng. Res., In press(2015).

25.
Eiji, I., Masako, N., Nobuyuki, K. and Kenji, K., "Synergy Effect of Ultrasonication and Salt Addition on Settling Behaviors of Activated Sludge," Sep. Purif. Technol., 144, 177-185(2015). crossref(new window)

26.
Cesar, O., Ana, P., Jose, V. G., Antonio, M. and Juan, A. C., "Influence of High Intensity Ultrasound Application on Mass Transport, Microstructure and Textural Properties of Pork Meat (Longissimus dorsi) Brined at Different NaCl Concentrations," J. Food Eng., 119, 84-93(2013). crossref(new window)