JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Moment Analysis (MA) of Lysozyme in Cation Exchange High Performance Liquid Chromatography (HPLC)
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Korean Chemical Engineering Research
  • Volume 54, Issue 4,  2016, pp.487-493
  • Publisher : The Korean Institute of Chemical Engineers
  • DOI : 10.9713/kcer.2016.54.4.487
 Title & Authors
Moment Analysis (MA) of Lysozyme in Cation Exchange High Performance Liquid Chromatography (HPLC)
Ko, Kwan Young; Kim, In Ho;
  PDF(new window)
 Abstract
The moment analysis of lysozyme was implemented using chromatograms that were obtained from weak cation exchange column in high performance liquid chromatography system. Three elution sodium phosphate buffers containing 1.0, 0.75, 0.5M sodium chloride were used. Experiments were conducted by varying flow rate, elution sodium chloride concentration, and lysozyme solute concentration. The general rate (GR) model was employed to calculate the first moment and the second moment. By plotting vs. ] equilibrium constants (K) were obtained from first moment analysis. Intra-particle diffusivity was obtained from theoretical plate number data. Based on the results of moment analysis, van Deemter plots were drawn in order to investigate the contributions of , , and to total Height Equivalent to a Theoretical Plate (HETP, ). The effect of intra-particle diffusion () was the most dominant factor contributing to HETP while external mass transfer () was negligible factor.
 Keywords
Moment Analysis;Iysozyme;
 Language
Korean
 Cited by
 References
1.
Otto, G., "Interpretation of Asymmetric Curves in Linear Chromatography," Anal. Chem., 43(14), 1934-1937(1971). crossref(new window)

2.
Miyabe, K., "Moment Analysis of Chromatographic Behavior in Reversed-phase Liquid Chromatography," J. Sep. Sci., 32, 757-770(2009). crossref(new window)

3.
Kim, H. M., Kim, A. R., Lee, C. S., and Kim, I. H., "Identification of Proteins in Egg White using Ion Exchange Cartridge and RP-HPLC," Korean Chem. Eng. Res., 50(4), 713-717(2012). crossref(new window)

4.
Ko, K. Y. and Kim, I. H., "The Effect of pH and Temperature on Lysozyme Separation in Ion-exchange Chromatography," Korean Chem. Eng. Res., 52(1), 98-105(2014). crossref(new window)

5.
Miyabe, K. and Suzuki, M., "Chromatography of Liquid-phase Adsorption on Octadecylsilyl-silica Gel," AIChE J., 38, 901-910(1992). crossref(new window)

6.
Suzuki, M., Adsorption engineering, Kodansha, Elsevier, Tokyo, Japan(1990).

7.
Liu, Z., Roininen, J., Pilkkinen, I., Saari, P., Sainio, T. and Alopaeus, V., "A New Moment Analysis Method to Estimate the Characteristic Parameters in Chromatographic General Rate Model," Computers and Chemical Engineering, 55, 50-60(2013). crossref(new window)

8.
Choi, D. Y. and Row, K. H., "Estimation of Kinetic and Rate Parameters of Peptides by Moment Analysis," J. Ind. Eng. Chem., 10, 1052-1057(2004).

9.
http://www.sanderkok.com/techniques/hplc/column_properties.html.

10.
Kestin, J., Khalifa, H. E. and Correia, R. J., "Tables of the Dynamic and Kinematic Viscosity of Aqueous NaCl Solutions in the Temperature Range $20-150^{\circ}C$ and the Pressure Range 0.1-35 MPa," J. Phys. Chem. Ref. Data, 10(1), 71-87(1981). crossref(new window)