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Moment Analysis (MA) of Lysozyme in Cation Exchange High Performance Liquid Chromatography (HPLC)
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  • Journal title : Korean Chemical Engineering Research
  • Volume 54, Issue 4,  2016, pp.487-493
  • Publisher : The Korean Institute of Chemical Engineers
  • DOI : 10.9713/kcer.2016.54.4.487
 Title & Authors
Moment Analysis (MA) of Lysozyme in Cation Exchange High Performance Liquid Chromatography (HPLC)
Ko, Kwan Young; Kim, In Ho;
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The moment analysis of lysozyme was implemented using chromatograms that were obtained from weak cation exchange column in high performance liquid chromatography system. Three elution sodium phosphate buffers containing 1.0, 0.75, 0.5M sodium chloride were used. Experiments were conducted by varying flow rate, elution sodium chloride concentration, and lysozyme solute concentration. The general rate (GR) model was employed to calculate the first moment and the second moment. By plotting vs. ] equilibrium constants (K) were obtained from first moment analysis. Intra-particle diffusivity was obtained from theoretical plate number data. Based on the results of moment analysis, van Deemter plots were drawn in order to investigate the contributions of , , and to total Height Equivalent to a Theoretical Plate (HETP, ). The effect of intra-particle diffusion () was the most dominant factor contributing to HETP while external mass transfer () was negligible factor.
Moment Analysis;Iysozyme;
 Cited by
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