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Therapeutic Effects of Sesamum Indicum Extracts on Atopic Dermatitis-Like Skin Lesions of NC/Nga Mouce
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 Title & Authors
Therapeutic Effects of Sesamum Indicum Extracts on Atopic Dermatitis-Like Skin Lesions of NC/Nga Mouce
Kim, Yun Hee; Lee, Hye Lim;
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 Abstract
Objectives The purpose of this study is to investigate the effects of Sesamum indicum extracted (SEI) on atopic dermatitis in an in-vitro and in-vivo experiment using a MC/9 murine mast cells and a NC/Nga mouse. Methods In-vitro experiment, IL-4, IL-5, IL-6, IL-13, TNF- and GM-CSF mRNA expression were evaluated by Real-time PCR, IL-13, MIP- production by ELISA and manifestations of NFAT-1, NFAT-2, c-jun, c-fos, NF- p65 transcription factors by western blotting. In-vivo experiment, we measured WBC, Eosinophil, Neutrophil, and serum IL-5, IL-13 in NC/Nga atopic dermatitis mouse, IL-5, IL-13, IFN-, IL-4 in the spleenocyte culture supernatant by ELISA, the absolute cell numbers of CD4+, CD8+, +Gr-1+CD11b, B220+CD23+ in the axillary lymph node (ALN), peripheral blood mononuclear cells (PBMCs) and dorsal skin tissue, IL-5, IL-13 by Real-time PCR, the distribution of tissue inflammation and cellular infiltration by H&E and toluidine blue. Results SEI decreased IL-4, IL-5, IL-6, IL-13, GM-CSF, TNF- mRNA expression, IL-13, MIP- production and the expression of transcription factors including NFAT-1, c-jun, NF- p65 in MC/9 murine mast cells. SEI orally administration decreased cell number of WBC, Eosinophil, the level of serum IgE, total cell number of ALN and dorsal skin tissue, absolute cell number of CD4+, CD8+, B220+CD23+ in the ALN. SEI orally administration also increased absolute cell number of CD8+/CD3+ and decreased Gr-1+/CD11b+ in PBMCs, decreased CD4+ in dorsal skin tissue, inhibited IL-5, IL-13 mRNA expression. Infiltration levels of inflammatory immune cells, mast cells and thickness of epidermis decreased in dorsal skin tissue. Conclusions SEI can regulate allergic inflammatory response suppressed the gene expression and production of cytokines that mediate allergic reactions, and will be able to be effectively utilized in the treatment of atopic dermatitis future.
 Keywords
胡麻子 (Sesamum indicum);NC/Nga mouse;Atopic dermatitis;DNCB;MC/9 murine mast cell;
 Language
Korean
 Cited by
 References
1.
Leung Donald YM. Atopic dermatitis: new insights and opportunities for therapeutic intervention. J Allergy Clin Immunol. 2000;105(5):860-76. crossref(new window)

2.
Jang SN, Kim KR, Yun MY, Kang SM, Moon MY. The effect of $\gamma$-PGA on NC/Nga mice, a mouse model for mite antigen-induced atopic dermatitis. Kor J Microbiol Biotechnol. 2010;38(1):53-63.

3.
Watanabe A, Mishima H, Renzi PM, Xu LJ, Hamid Q, Martin JG. Transfer of allergic airway responses with antigen-primed CD4+ but not CD8+T cells in brown Norway rats. J Clin Invest. 1995;96(3):1303-10. crossref(new window)

4.
Korean dermatological association of Korea's Colleges of Medicine. Textbook of dermatology. Seoul: Daehanuihak. 2014:75, 91-2.

5.
Herbology Association of Korea's Colleges of Oriental Medicine. Textbook of herbology. Seoul: Younglimsa. 2011:666-7.

6.
Huh J. Donguibogam. Seoul: Bubin Publishers. 2005:883, 1349-50, 2740-1.

7.
Hwang MC, Chai BY. Experimental studies on the effect of HoMaSan. J Kyung Hee Univ Med Cent. 1987;10:563-77.

8.
Seo HS, Roh SS. The study on the inhibitory effect of allery by SoPungSan. J Korean Medical Ophthalmol Otolaryngol Dermatol. 2000;13(1):22-9.

9.
Ryu JC, Lee JG, Ku Sk, Jee SY. Effect of Sopung-san extracts on the passive cutaneous anaphylaxis of wistar rats (Type I Allergic Dermatitis). J Korean Medical Ophthalmol Otolaryngol Dermatol. 2009;22(3):1-10.

10.
Park SN, Jung HS, Lee JY. Effect of Sopungsan in BALB/c mouse model of atopic dermatitis. J Kyung Hee Univ Med Cent. 2004;20(2):129-41.

11.
Lee JS, Seo HS, Roh SS. The stydy on the anti-allergic effect of SoPungSan-Gami. J Korean Medical Ophthalmol Otolaryngol Dermatol. 2001;14(2):9-20.

12.
Na HU, Jung HS, Lee GG. Anti-allergic property of Chungjokupye-tang. Korean J Orient Physiol Pathol. 2003;17(6):1376-82.

13.
Guk YB, Kum SC, Park SD, Park SG, Seo BY, Seo YB, Sin SS, Lee SY, Lee JC, Lee C, Jung JG, Joo YS, Choi HY. Bangjehak. Seoul: Younglimsa. 2008;439-40.

14.
Smith D.E., Salerno J.W. Selective growth inhibition of a human malignant melanoma cell line by sesame oil in vitro. PLEFA. 1992;46(2):145-50.

15.
Nahar L., Rokonuzzaman. Investigation of the analgesic and antioxidant activity from an ethanolic extract of seeds of Sesamum indicum. Pak J Biol Sci. 2009;12(7):595-8. crossref(new window)

16.
Sirato-Yasumoto S M J, Katsuta Y, Okuyama Y, Takahashi Ide T. Effect of sesame seeds rich in sesamin and sesamolin on fatty acid oxidation in rat liver. J Agri Food Chem. 2001;49:2647-51. crossref(new window)

17.
Ahn SG, Pack BD, Won JH, Sung YY, Cheon SH. Atopic dermatitis for the opening doctor. Seoul: Pacific Publisher Co. 2007:13-82.

18.
Bischoff SC. Role of mast cells in allergic and non-allergic immune responses comparison of human and murine data. Nat Rev Immunol. 2007;7(2):93-104. crossref(new window)

19.
Korean dermatological association of Korea's Colleges of Medicine. Textbook of dermatology. Seoul: Daehanuihak. 2014:75, 91-2.

20.
Lee IS, Dat NT, Cai XF, Shen GH, Kim YH. Inhibitory effects of natural products against NFAT (nuclear factor of activated T cells) transcription factor. Korean J Pharmacogn. 2003;34(2):150-5.

21.
Lewin I, Nechushtan H, Ke Q, Razin E. The role played by protein kinase C and the possible involvement of Fos interacting protein. Blood. 1993;82(1):3745-51.

22.
Park GY, Lee SH, Hwang BB, Im JJ, Lee CT, Kim YH, Han SG, Shim YS, You CG. Pro-inflammatory cytokine expression through NF-$\kappa$B/I$\kappa$B pathway in lung epithelial cells. Tuberc Respir Dis. 2000;49(3):332-42. crossref(new window)

23.
Vestergaard C, Yoneyama H, Matsushima K. The NC/Nga mouse: A model for atopic dermatitis. Mol Med Today. 2000;5(1):209-10.

24.
Bellanti JA. Cytokines and allergic diseases: clinical aspects. Allergy Asthma Proc. 1998;19(6):337-41. crossref(new window)

25.
Yawalkar N, Uguccioni M, Scharer J, Braunwalder J, Karlen S, Dewald B, Braathen LR, Baggiolini M. Enhanced expression of eotaxin and CCR3 in atopic dermatitis. J Invest Dermatol. 1999;113(1):43-8. crossref(new window)

26.
Ryouji Y, Haruko N, Yutaka I, Toshihiro A, Takao A, Masato K. Development of atopic dermatitis-like skin lesions in STAT6-deficient NC/Nga mouse. J Immunol. 2002;168(1):2020-7. crossref(new window)

27.
Bronte V, Wang M, Overwijk WW, Surman DR, Pericle F, Rosenberg SA, Restifo NP. Apoptotic death of CD8+T lymphocytes after immunization. J Immunol. 1998;161 (10):5313-20.

28.
Angulo I, Heras FG, Garcia-Bustos JF, Gargallo D, Munoz-Fernandez A, Fresno M. Nitric oxide-producing CD11b (+)Ly-6G (Gr-1) (+)CD31 (ER-MP12) (+) cells in the spleen of cyclophosphamide-treated mice:implications for T-cell responses in immunosuppressed mice. Blood. 2000;95(1):212-20.

29.
Dorshkind K, Narayanan R, Landreth KS. Regulatory cells and cytokines involved in primary B lymphocyte production. Adv Exp Med Biol. 1992;323:119-23. crossref(new window)

30.
Rosenwasser LJ, Busse WW, Lizambri RG, Olejnik TA, Totoritis MC. Allergic asthma and an anti- CD23+mAb (IDEC-152). J Allergy Clin Immunnol. 2003;112(3):563-70. crossref(new window)