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Activating the Proliferation of Keratinocyte Stem Cells by Paeonol, a Compound from Natural Herb
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 Title & Authors
Activating the Proliferation of Keratinocyte Stem Cells by Paeonol, a Compound from Natural Herb
Kim, Do Hyung; Kim, Hyo Jin; Yeo, Hyerin; Lee, Cheon Goo; Lee, Sang Hwa;
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 Abstract
Epidermis is continuously regenerated by keratinocyte stem cells (KSCs) residing in basement membrane, which is critical to the survival of an organism. KSCs are believed to persist during the whole lifetime and generate an enormous number of keratinocytes, required for the maintenance of epidermis, through transit amplifying cells dividing definite times until they become differentiated. In this report, we have developed a phenolic compound, paeonol, purified from Moutan Cortex, as a KSC proliferation activator, by screening about 350 herbal compounds. The cell proliferation activation by paeonol is specific for KSC not for keratinocyte, and no significant difference in the expression of p63 protein, a KSC marker, in KSCs treated with paeonol was observed in FACS analysis with anti-p63 antibody. In the colony forming assay, paeonol-treated KSC showed improved colony forming activity more than 1.3 fold. In addition, the result of PCR array shows that the activity of paeonol is through several signal pathways involving stem cell functions. These results suggest that paeonol could enhance KSC proliferation activity without reduction in stemness and could be applied to cosmetics as a KSC activating ingredient.
 Keywords
anti-aging;paeonol;keratinocyte stem cell;p63 protein;cosmetics;
 Language
Korean
 Cited by
 References
1.
R. M. Lavker and T. T. Sun, Epidermal stem cells: properties, markers, and location, Proc. Natl. Acad. Sci. USA, 97(25), 13473 (2000). crossref(new window)

2.
L. Alonso and E. Fuchs, Stem cells of the skin epithelium, Proc. Natl. Acad. Sci. USA, 100(suppl1), 11830 (2003). crossref(new window)

3.
C. Blanpain and E. Fuchs, Epidermal stem cells of the skin, Annu. Rev. Cell. Dev. Biol., 22, 339 (2006). crossref(new window)

4.
A. Webb, A. Li, and P. Kaur, Location and phenotype of human adult keratinocyte stem cells of the skin, Differentiation, 72(8), 387 (2004). crossref(new window)

5.
P. Kaur, Interfollicular epidermal stem cells: identification, challenges, potential, J. Invest. Dermatol., 126(7), 1450 (2006). crossref(new window)

6.
J. R. Bickenbach, M. M. Stern, K. L. Grinnell, A. Manuel, and S. Chinnathambi, Epidermal stem cells have the potential to assist in healing damaged tissues, J. Investig. Dermatol. Symp. Proc., 11(1), 118 (2006). crossref(new window)

7.
M. Senoo, F. Pinto, C. P. Crum, and F. McKeon, p63 is essential for the proliferative potential of stem cells in stratified epithelia, Cell, 129(3), 523 (2007). crossref(new window)

8.
G. Pellegrini, E. Dellambra, O. Golisano, E. Martinelli, I. Fantozzi, S. Bondanza, D. Ponzin, F. McKeon, and M. D. Luca, p63 identifies keratinocyte stem cells, Proc. Natl. Acad. Sci. USA, 98(6), 3156 (2001). crossref(new window)

9.
X. Su, M. Paris, Y. J. Gi, K. Y. Tsai, M. S. Cho, Y. L. Lin, J. A. Biernaskie, S. Sinha, C. Prives, L. H. Pevny, F. D. Miller, and E. R. Flores, TAp63 prevents premature aging by promoting adult stem cell maintenance, Cell Stem Cell, 5(1), 64 (2009). crossref(new window)

10.
N. Gago, V. Perez-Lopez, J. P. Sanz-Jaka, P. Cormenzana, I. Eizaguirre, A. Bernad, and A. Izeta, Age-dependent depletion of human skin-derived progenitor cells, Stem Cells, 27(5), 1164 (2009). crossref(new window)

11.
H. R. Choi, Y. A. Kang, J. I. Na, S. Y. Huh, C. H. Hur, K. H. Kim, and K. C. Park, Oligosaccharides of hyaluronic acid increased epidermal cell stemness by modulation of integrin expression, J. Cosmet. Dermatol., 11(4), 290 (2012). crossref(new window)