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Development of Quantitative Analytical Method for Isoflavonoid Compounds from Fruits of Cudrania Tricuspidata
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 Title & Authors
Development of Quantitative Analytical Method for Isoflavonoid Compounds from Fruits of Cudrania Tricuspidata
Yoon, Sun Young; Kim, Seok Ju; Sim, Su Jin; Lee, Hak-Ju;
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 Abstract
In this study, an analytical method to evaluate the quality of isoflavonoid compounds purified and isolated from the fruits of Cudrania tricuspidata was developed and validated using Ultra Performance Liquid Chromatography (UPLC). The fruits of C. tricuspidata were extracted with methanol and further fractioned with n-hexane, ethyl acetate and water. The resulting ethyl acetate extract separated into four isoflavonoid compounds by a combination of silica gel and sephadex LH-20 column chromatography. The structures of the compounds were elucidated as alpinumisoflavone, 6,8-diprenyl genistein, 6,8-diprenyl orobol, 4`-O-methylalpinumisoflavone by various techniques such as UV-Vis, ESI-MS, and spectroscopy. Finally, a method to characterize the compounds was developed by using the UPLC equipped with a column and a gradient mobile phase system consisting of 2% acetic acid in water (solvent A) and 2% acetic acid in methanol (solvent B). The developed method was validated with the parameters such as selectivity, linearity, limit of detection, limit of quantitation, accuracy, and precision, which are defined by the ICH (International Conference on Harmonization). Using the validated method, the compounds in the fruits harvested in different months were also quantitatively analyzed. We propose this approach this approach can readily be utilized as an efficient evaluation method to quantify the extracts of C. tricuspidata.
 Keywords
Cudrania tricuspidata;alpinumisoflavone;6,8-diprenyl genistein;6,8-diprenyl orobol;4`-O-methyl-alpinumisoflavone;ultra performance liquid chromatography;validation;
 Language
Korean
 Cited by
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