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Quantification of Reproductive Output of the Butter Clam, Saxidomus purpuratus(Sowerby, 1852) Using Enzyme-Linked Immunosorbent Assay (ELISA)
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  • Journal title : Ocean and Polar Research
  • Volume 25, Issue 3,  2003, pp.249-256
  • Publisher : Korea Institute of Ocean Science & Technology
  • DOI : 10.4217/OPR.2003.25.3.249
 Title & Authors
Quantification of Reproductive Output of the Butter Clam, Saxidomus purpuratus(Sowerby, 1852) Using Enzyme-Linked Immunosorbent Assay (ELISA)
Park, Kyung-Il; Choi, Jin-Woo; Choi, Kwang-Sik;
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 Abstract
An immunological method was developed in this study to quantify reproductive output of the female butter clam, Saxidomus purpuratus. A clam egg-specific polyclonal antibody was developed using the purified butter clam egg as an antigen. An indirect Enzyme-Linked Immunosorbent Assay (ELISA) was used in quantitative measurement of the eggs. Size of the butter clam eggs ranged from in histology or in intact eggs. The predominant egg constituent was protein (37.44%), followed by lipids (11.40%) and carbohydrates (9.68%). The SDS-PAGE showed that the egg proteins are composed of several peptides of molecular weights consisting of 247, 200, 99, 91, 54 and 47 kDa. ELISA indicated that the clams collected from Geoje Island in May 2002 produced 8.2 to 26.8% of their body weight as eggs or 9,307,309 to 31,156,333 with a mean of 16,931,893 eggs per individual clam. The results of this study thus suggest that indirect ELISA using rabbit anti-clam egg IgG as a primary antibody is a rapid, affordable and sensitive method to assess reproductive output of 5. purpuratus and possibly other bivalves using a small amount of eggs.
 Keywords
Saxidomus purpuratus;immune-assay;reproduction;reproductive output;egg;fecundity;ELISA;Korea;
 Language
English
 Cited by
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Application of a Gene-Based Population Dynamics Model to the Optimal Egg Size Problem: Why Do Bivalve Planktotrophic Eggs Vary in Size?, Journal of Shellfish Research, 2011, 30, 2, 403  crossref(new windwow)
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