Advanced SearchSearch Tips
Quantification of Reproductive Output of the Butter Clam, Saxidomus purpuratus(Sowerby, 1852) Using Enzyme-Linked Immunosorbent Assay (ELISA)
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Ocean and Polar Research
  • Volume 25, Issue 3,  2003, pp.249-256
  • Publisher : Korea Institute of Ocean Science & Technology
  • DOI : 10.4217/OPR.2003.25.3.249
 Title & Authors
Quantification of Reproductive Output of the Butter Clam, Saxidomus purpuratus(Sowerby, 1852) Using Enzyme-Linked Immunosorbent Assay (ELISA)
Park, Kyung-Il; Choi, Jin-Woo; Choi, Kwang-Sik;
  PDF(new window)
An immunological method was developed in this study to quantify reproductive output of the female butter clam, Saxidomus purpuratus. A clam egg-specific polyclonal antibody was developed using the purified butter clam egg as an antigen. An indirect Enzyme-Linked Immunosorbent Assay (ELISA) was used in quantitative measurement of the eggs. Size of the butter clam eggs ranged from in histology or in intact eggs. The predominant egg constituent was protein (37.44%), followed by lipids (11.40%) and carbohydrates (9.68%). The SDS-PAGE showed that the egg proteins are composed of several peptides of molecular weights consisting of 247, 200, 99, 91, 54 and 47 kDa. ELISA indicated that the clams collected from Geoje Island in May 2002 produced 8.2 to 26.8% of their body weight as eggs or 9,307,309 to 31,156,333 with a mean of 16,931,893 eggs per individual clam. The results of this study thus suggest that indirect ELISA using rabbit anti-clam egg IgG as a primary antibody is a rapid, affordable and sensitive method to assess reproductive output of 5. purpuratus and possibly other bivalves using a small amount of eggs.
Saxidomus purpuratus;immune-assay;reproduction;reproductive output;egg;fecundity;ELISA;Korea;
 Cited by
Quantification of eggs and sperm in the Black-lip pearl oyster Pinctada margaritifera using an enzyme-linked immunosorbent assay (ELISA), Fisheries Science, 2014  crossref(new windwow)
Seasonal Changes in Reproduction and Biochemical Composition of the Cockle,Fulvia muticaReeve (1884), in Cheonsu Bay off the West Coast of Korea, Journal of Shellfish Research, 2011, 30, 1, 95  crossref(new windwow)
Quantitative assessment of reproductive effort of the Manila clamRuditapes philippinarumin a lagoon on Jeju Island (Korea) using enzyme-linked immunosorbent assay, Invertebrate Reproduction & Development, 2013, 57, 4, 316  crossref(new windwow)
The distribution, size, and age composition, and growth of Saxidomus purpurata (Sowerby, 1852) (Bivalvia: Veneridae) in Vostok Bay, the Sea of Japan, Russian Journal of Marine Biology, 2014, 40, 5, 375  crossref(new windwow)
Application of a Gene-Based Population Dynamics Model to the Optimal Egg Size Problem: Why Do Bivalve Planktotrophic Eggs Vary in Size?, Journal of Shellfish Research, 2011, 30, 2, 403  crossref(new windwow)
Generation time and the stability of sex-determining alleles in oyster populations as deduced using a gene-based population dynamics model, Journal of Theoretical Biology, 2011, 271, 1, 27  crossref(new windwow)
Temporal dynamics of amino and fatty acid composition in the razor clam Ensis siliqua (Mollusca: Bivalvia), Helgoland Marine Research, 2014, 68, 4, 465  crossref(new windwow)
Application of enzyme-linked immunosorbent assay for studying of reproduction in the Manila clam Ruditapes philippinarum (Mollusca: Bivalvia), Aquaculture, 2004, 241, 1-4, 667  crossref(new windwow)
Ahn, S.-H. 2001. Annual reproductive cycle of Saxidomus purpuratus (Sowerby, 1852) (Bivalvia: Veneridae) in Sacheon Bay, Korea. M.S. Thesis, Pukyung Nat’l Univ., Busan, Korea. (in Korean)

Bligh, E.G. and W.J. Dyer. 1959. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol., 37, 911-917. crossref(new window)

Ceballos-Vazquez, B.P., M. Arellano-Martinez, F. Garcia-Dominguez, and M. Villalejo-Fuerte. 2000. Reproductive cycle of the rugose pen shell, Pinna rugosa Sowerby, 1835 (Mollusca: bivalvia) from Bahia Concepcion, Gulf of California and its relation to temperature and photoperiod. J. Shellfish Res., 19, 95-99.

Choi, K.-S., D.H. Lewis, E.N. Powell, and S.M. Ray. 1993. Quantitative measurement of reproductive output in the American oyster, Crassostrea virginica (Gmelin), using an enzyme-linked immunosrobent assay (ELISA). Aquacult. Fish. Manage., 24, 375-398.

Choi, K.-S., E.N. Powell, D.H. Lewis, and S.M. Ray. 1994. Instantaneous reproductive effort in female American oysters, Crassostrea virginica, measured by a new immunoprecipitation assay. Biol. Bull., 186, 41-61. crossref(new window)

Chung, E.-Y., S.B. Hur, Y.-B. Hur, and J.S. Lee. 2001. Gonadal maturation and artificial spawning of the Manila clam Ruditapes philippinarum (Bivalvia: Veneridae), in Komso Bay, Korea. J. Fish. Sci. Tech., 4, 208-218.

Chung, E.-Y. and B.-S. Kim. 1994. Histological and ultrastructural studies on gonadal development and germ cell development of the purplish Washington clam, Saxidomus purpuratus (Sowerby). Bull. Coastal Res., Kunsan Nat’l Univ. 6, 1-15.

Davis, H.C. and P.E. Chanley. 1956. Spawning and egg production of oysters and clams. Biol. Bull., 110, 117-128. crossref(new window)

Deslous-Paoli, J.-M. and M. Heral. 1988. Biochemical composition of energy value of Crassostrea gigas (Thunberg) cultured in the Bay of Marennes-Oleron. Aquat. Living Resources, 1, 239-249. crossref(new window)

Dietrich, A.M., T.M.C. LaBreche, and N. Shepherd. 2002. A flow-through chamber for toxicity testing of 80-200 ${\mu}m$ organisms. Water Res., 36, 2002-2010. crossref(new window)

Fuchs, S. and M. Sela. 1979. Immunoadsorbents. p. 10.1-10.6. In: Handbook of Experimental Immunology. Vol. 1, Immunochenistry, ed. by D.M. Weir. Blackwell, Philadelphia.

Galinou-Mitsoudi, S. and A.I. Sinis. 1994. Reproductive cycle and fecundity of the Date mussel Lithophaga lithophaga (Bivalvia: Mytilidae). J. Moll. Stud., 60, 371-385. crossref(new window)

Galsoff, P.S. 1964. The American oyster Crassostrea virginica Gmelin. US Fish and Wildlife Serv. Fisher. Bull., 64, 1-480.

Hyun, K.-H., I.-C. Pang, J.M. Klinck, K.-S. Choi, J.-B. Lee, E.N. Powell, E.E. Hofmann, and E.A. Bochenek. 2001. The effect of food composition on Pacific oyster Crassostrea gigas (Thunberg) growth in Korea: a modeling study. Aquaculture, 199, 41-62. crossref(new window)

Kang, S.-G., K.-S. Choi, A.A. Bulgakov, Y. Kim, and S.-Y. Kim. 2003. Enzyme-linked immunosorbent assay (ELISA) used in quantification of reproductive output in the pacific oyster, Crassostrea gigas, in Korea. J. Exp. Mar. Biol. Ecol., 282, 1-21. crossref(new window)

Kim, A.-Y. 1971. Ecological studies on the propagation of Saxidomus purpuratus (Sowerby). Bull. Kor. Fish. Soc., 4, 92-98. (in Korean)

Kim, S.K., K.Y. Park, G.N. Jang, D.J. Kim, and H.C. Seo. 2001. Studies on the ecological aspect and gametogenesis of Saxidomus purpuratus (Sowerby) in Yellow Sea area. Bull. NFRDI, 59, 152-158. (in Korean)

Laemmli, V.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685. crossref(new window)

Lee, R.F. and P.B. Heffernan. 1991. Lipids and proteins in eggs of eastern oysters (Crassostrea virginica (Gmelin, 1791)) and northern quahogs (Mercenaria mercenaria (Linnaeus, 1758)). J. Shellfish Res., 10, 203-206.

Loosanoff, V.L. and H.C. Davis. 1963. Rearing of bivalve mollusks. Advances in Mar. Biol., 1, 1-136. crossref(new window)

Lucas, A. 1982. Evaluation of reproductive effort in bivalve molluscs. Malacologia, 22, 183-187.

Marsh, J.B. and D.B. Weinstein. 1966. Simple charring method for determination of lipids. J. Lipid Res., 7, 574-576.

Massapina, C., S. Joaquim, D. Matias, and N. Devauchelle. 1999. Oocyte and embryo quality in Crassostrea gigas (Portuguese strain) during a spawning period in Algarve, South Portugal. Aquat. Living Resources, 12, 327-333. crossref(new window)

MOMAF. 2001. Development of optimal technology for sustaining production in shellfish farm. (Final report) (in Korean)

Ouchterlony, O. and L.A. Nilsson. 1978. Immunodiffusion and immunoelectrophoresis. p. 19.1-19.44. In: Handbook of Experimental Immunology. Vol. 1, ed. by D.M. Weir. Blackwell, Oxford, England.

Peterson, C.H. 1983. A concept of quantitative reproductive senility: application to the hard clam, Mercenaria mercenaria (L.). Oecologia, 58, 164-168. crossref(new window)

Pillay, T.V.R. 1990. Aquaculture principles and practices. Cambridge Univ. Press, UK. 575 p.

Park, K.-I. and K.-S. Choi. (in preparation) Study on the reproduction of the Manila clam Ruditapes philippinarum. I. Quantification of the reproductive output using Enzyme Linked-Immunosorbent Assay (ELISA).

Powell, E.N., J.M. Klink, E.E. Hofmann, and S.M. Ray. 1994. Modeling oyster populations. IV: rates of mortality, population crashes, and management. Fish. Bull., 92, 347-373.

Shafir, S., M. Ovadia, and M. Tom. 1992. In vivo incorporation of labeled methionine into proteins, vitellogenin, and vitellin in females of the shrimp Penaeus semisulcatus de Haan. Biol. Bull., 183, 242-247. crossref(new window)

South Sea Fisheries Institute, NFRDI. 2002. Fisheries resources. Available from WWW: [cited 2003-04-30].

Taylor, K.A. 1995. A modification of the phenol sulfuric acid method and total sugar determination. Appl. Biochem. & Biotechnol., 53, 207-214. crossref(new window)

Tirado, C. and C. Salas. 1998. Reproduction and fecundity of Donax trunculus L., 1758 (Bivalvia: Donacidae) in the littoral of Malaga (Southern Spain). J. Shellfish Res., 17, 169-176.