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Phylogenetic Analysis of Culturable Arctic Bacteria
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  • Journal title : Ocean and Polar Research
  • Volume 26, Issue 1,  2004, pp.51-58
  • Publisher : Korea Institute of Ocean Science & Technology
  • DOI : 10.4217/OPR.2004.26.1.051
 Title & Authors
Phylogenetic Analysis of Culturable Arctic Bacteria
Lee, Yoo-Kyung; Kim, Hyo-Won; Cho, Kyeung-Hee; Kang, Sung-Ho; Lee, Hong-Kum; Kim, Yea-Dong;
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We isolated and identified culturable Arctic bacteria that had inhabited soils around the Korean Arctic Research Station Dasan located at Ny-Alsund, Svalbard, Norway . The collected soils were diluted in distilled water; the diluted soil-water was spread on 3M petri-films at Dasan Station. The petri-films were transported to the laboratory at KORDI, and cultured at . Colonies grown on the petri-films were subsequently cultured on nutrient agar plates at every 7 days. The pure colonies were inoculated into nutrient liquid media, genomic DNA was extracted, and phylogenetic analysis was performed on the basis of 165 rDNA sequences. A total of 227 strains of bacteria were isolated. Among them, 16S rDNA sequences of 185 strains were identical with those of known strains isolated in this study, and 42 strains were finally identified. Phylogenetic analysis using 16S rDNA indicated that the 30 strains belonged to Pseudomonas, 7 strains to Arthrobacter, two strains to Flavobacterium, and the remaining to Achromobacter, Pedobacter, and Psychrobacter. Among the 42 strains, 14 bacteria produced protease: they were 6 strains of Pseudomonax, 4 strains of Arthrobater, an Achromobacter strain, 2 strains of Flavobacterium, and a Pedohacter strain. We expect these Arctic bacteria can be used for screening to develop new industrial enzymes that are active at low temperatures.
Arctic bacteria;Pseudomonas;Arthrobacter;Flavobacterium;Achromobacter;Pedobacter;Psychrobacter;
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