Advanced SearchSearch Tips
Studies on Screening of Seaweed Extracts for Peroxynitrite and DPPH Radical Scavenging Activities
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Ocean and Polar Research
  • Volume 26, Issue 1,  2004, pp.59-64
  • Publisher : Korea Institute of Ocean Science & Technology
  • DOI : 10.4217/OPR.2004.26.1.059
 Title & Authors
Studies on Screening of Seaweed Extracts for Peroxynitrite and DPPH Radical Scavenging Activities
Lee, Hee-Jung; Kim, You-Ah; Park, Ki-Eui; Jung, Hyun-Ah; Yoo, Jong-Su; Ahn, Jong-Woong; Lee, Burm-Jong; Seo, Young-Wan;
  PDF(new window)
As a part of our search for novel antioxidants from the seaweeds, we have investigated radical scavenging effect for their crude extracts using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, authentic peroxynitrite, and 3-morpholinsydnonimine (SIN-1), a peroxynitrite-generating species in vitro. Thirty-four seaweeds were screened for and DPPH radical scavenging activities. A potent inhibitory effect against peroxynitrite generated by SIN-1 at of methanol extracts was observed in order of Ishige okamurae(95.3%), Sargassum hemiphyllum(90.2%), Symphyocladia latiuscula(89.6%), Porphyra suborbiculata(86.7%), and Gelidium amamsii(85.9%), Also, a significant scavenging effect against direct authentic peroxynitrite was revekaled for methanol extracts of Ishige okamurae(66.2%) and Sargassum hemiphyllum(55.2%) and the acetone/methylene chloride(1:1) extract of Gigatina tenella (61.0%). In our measurement for evaluating the capacity to scavenge the stable free radical of DPPH, acetone/methylene chloride(1:1) extracts of Symphyocladia latiuscula, Gloiopeltis furcata, and Sargassum thunbergii and the methanol extract of Sargassum sp. showed an inhibitory potency of 85.8%, 82.8%, 74.1%, and 64.0%, respectively.
seaweeds;DPPH radical scavenging effect;peroxynitrite scavenging effect;
 Cited by
Antioxidant Efficacy of Extracts from a Variety of Seaweeds in a Cellular System,Kim, You-Ah;Kong, Chang-Suk;Um, Young-Ran;Lee, Jung-Im;Nam, Taek-Jeong;Seo, Young-Wan;

Ocean Science Journal, 2008. vol.43. 1, pp.31-37
Antioxidants from macroalgae: potential applications in human health and nutrition,Cornish, M. Lynn;Garbary, David J.;

ALGAE, 2010. vol.25. 4, pp.155-171 crossref(new window)
Screening of Korean Marine Plants Extracts for Inhibitory Activity on Protein Tyrosine Phosphatase 1B,Lee, Hee-Jung;Kim, You-Ah;Lee, Jung-Im;Lee, Burm-Jong;Seo, Young-Wan;

Journal of Applied Biological Chemistry, 2007. vol.50. 2, pp.74-77
Photo-protective properties of Lomentaria hakodatensis yendo against ultraviolet B radiation-induced keratinocyte damage, Biotechnology and Bioprocess Engineering, 2012, 17, 6, 1223  crossref(new windwow)
Antioxidant properties ofErigeron annuus extract and its three phenolic constituents, Biotechnology and Bioprocess Engineering, 2006, 11, 1, 13  crossref(new windwow)
Antioxidants from macroalgae: potential applications in human health and nutrition, ALGAE, 2010, 25, 4, 155  crossref(new windwow)
Anti-obesity effects of seaweeds of Jeju Island on the differentiation of 3T3-L1 preadipocytes and obese mice fed a high-fat diet, Food and Chemical Toxicology, 2016, 90, 36  crossref(new windwow)
In vitro antioxidant properties of crude extracts and compounds from brown algae, Food Chemistry, 2013, 138, 2-3, 1764  crossref(new windwow)
Antioxidant efficacy of extracts from a variety of seaweeds in a cellular system, Ocean Science Journal, 2008, 43, 1, 31  crossref(new windwow)
Screening of Korean marine plants for their inhibitory effect on histamine release from RPMCin vitro, Biotechnology and Bioprocess Engineering, 2006, 11, 1, 80  crossref(new windwow)
Ali, M.S., M. Jahangir, M. Saleem, M.K. Pervez, S. Hameed, and V.U. Ahmad. 2000. Metabolites of marine algae collected from Karachi-coasts of Arabian Sea. Natural Product Sciences, 6(2), 61-65.

Ancerewicz, J., E. Migliavacca, P.A. Carrupt, B. Testa, F. Bree, R. Zini, J.P. Tillement, S. Labidalle, D. Guyot, A.M. Chauvet-Monges, A. Crevat, and A.L. Ridant. 1998. Structure-property relationships of trimetazidine derivatives and model compounds as potential antioxidants. Free Radical Biology & Medicine, 25(1), 113-120. crossref(new window)

Blois, M.S. 1958. Antioxidant determinations by the use of a stable free radical. Nature, 26, 1199-1200.

Chung, H.Y., H.R. Choi, H.J. Park, J.S. Choi, and W.C. Choi. 2001. Peroxynitrite scavenging and cytoprotective activity of 2,3,6-tribromo 4,5-dihydroxybenzyl methyl ether from the marine alga Symphyocladia latiuscula. J. Agric. Food Chem., 49, 3614-3621. crossref(new window)

Hoppe, H.A. 1979. Marine algae in pharmaceutical science. Walter de Gruyter, Berlin-New York, 139 p.

Kooy, N.W., J.A. Royall, H. Ischiropoulos, and J.S. Beckman. 1994. Peroxynitrite-mediated oxidation of dihydrorhodamine 123. Free Radical Biology & Medicine, 16, 149-156. crossref(new window)

Pryor, W. and G. Squadrito. 1995. The chemistry of peroxynitrite: a product from the reaction of nitric oxide with the superoxide anion. A. J. Physiol., 264, 699-722.

Reiter, R.J., D.X. Tan, and S. Burkhardt. 2002. Reactive oxygen and nitrogen species and cellular and organismal decline : amelioration with melatonin. Mechanism of Aging and Development, 123, 1007-1019. crossref(new window)

Ruperez, P. 2002. Mineral content of edible marine seaweeds. Food Chemistry, 79, 23-26. crossref(new window)

Sanchez-Machado, D.I., J. Lopez-Cervantes, J. Lopez-Hernandez, and P. Paseiro-Losada. 2003. Fatty acids, total lipid, protein and ash contents of processed edible seaweeds. Food Chemistry, 85(3), 439-444. crossref(new window)

Sandoval M., R.A. Ronzio, D.N. Muanza, D.A. Clark, and M.J.S. Miller. 1997. Peroxynirite-induced apoptosis in epithelial(T84) and macrophage(RAW 264.7) cell lines : Effect of legume-derived polyphenols(phytolens) NITRIC OXIDE. Biology and Chemistry, 1(6), 476-483.

Scheuer, P.J. 1990. Bioorganic Marine Chemistry. Springer-Verlag, New York. 123 p.

Thomson L., A. Denicola, and R. Radi. 2003. The trypanothionethiol system in Trypanosoma cruzi as a key antioxidant mechanism against peroxynitrite-mediated cytotoxicity. Archives of Biochemistry and Biophysics, 412, 55-64. crossref(new window)