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Studies on Screening of Seaweed Extracts for Peroxynitrite and DPPH Radical Scavenging Activities
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  • Journal title : Ocean and Polar Research
  • Volume 26, Issue 1,  2004, pp.59-64
  • Publisher : Korea Institute of Ocean Science & Technology
  • DOI : 10.4217/OPR.2004.26.1.059
 Title & Authors
Studies on Screening of Seaweed Extracts for Peroxynitrite and DPPH Radical Scavenging Activities
Lee, Hee-Jung; Kim, You-Ah; Park, Ki-Eui; Jung, Hyun-Ah; Yoo, Jong-Su; Ahn, Jong-Woong; Lee, Burm-Jong; Seo, Young-Wan;
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As a part of our search for novel antioxidants from the seaweeds, we have investigated radical scavenging effect for their crude extracts using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, authentic peroxynitrite, and 3-morpholinsydnonimine (SIN-1), a peroxynitrite-generating species in vitro. Thirty-four seaweeds were screened for and DPPH radical scavenging activities. A potent inhibitory effect against peroxynitrite generated by SIN-1 at of methanol extracts was observed in order of Ishige okamurae(95.3%), Sargassum hemiphyllum(90.2%), Symphyocladia latiuscula(89.6%), Porphyra suborbiculata(86.7%), and Gelidium amamsii(85.9%), Also, a significant scavenging effect against direct authentic peroxynitrite was revekaled for methanol extracts of Ishige okamurae(66.2%) and Sargassum hemiphyllum(55.2%) and the acetone/methylene chloride(1:1) extract of Gigatina tenella (61.0%). In our measurement for evaluating the capacity to scavenge the stable free radical of DPPH, acetone/methylene chloride(1:1) extracts of Symphyocladia latiuscula, Gloiopeltis furcata, and Sargassum thunbergii and the methanol extract of Sargassum sp. showed an inhibitory potency of 85.8%, 82.8%, 74.1%, and 64.0%, respectively.
seaweeds;DPPH radical scavenging effect;peroxynitrite scavenging effect;
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