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Isolation of Protease-Producing Arctic Marine Bacteria
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  • Journal title : Ocean and Polar Research
  • Volume 27, Issue 2,  2005, pp.215-219
  • Publisher : Korea Institute of Ocean Science & Technology
  • DOI : 10.4217/OPR.2005.27.2.215
 Title & Authors
Isolation of Protease-Producing Arctic Marine Bacteria
Lee, Yoo-Kyung; Sung, Ki-Cheol; Yim, Joung-Han; Park, Kyu-Jin; Chung, Ho-Sung; Lee, Hong-Kum;
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We isolated and identified three protease-producing bacteria that had inhabited the region around the Korean Arctic Research Station Dasan located at Ny-Alesund, Svalbard, Norway . Biofilms were collected from the surface of a floating pier and from dead brown algae in a tide pool near the seashore. The biofilm samples were transported to the Korea Polar Research Institute (KOPRI) under frozen conditions, diluted in sterilized seawater, and cultured on Zobell agar plates with 1% skim milk at . Three clear zone forming colonies were selected as protease-producing bacteria. Phylogenetic analysis based on 16S rDNA sequences showed that these three stains shared high sequence similarities with Pseudoalteromonas elyakovii, Exiguobacterium oxidotofewm Pseudomonas jessenii, respectively. We expect these Arctic bacteria may be used to develop new varieties of protease that are active at low temperatures.
Arctic;Marine bacteria;Protease;Exiguobacterium;Pseudoalteromonas;Pseudomonas;
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