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Identification of Ruditapes philippinarum and Meretrix lusoria Larvae Using Single Cell PCR Analysis and Microscopic Observation
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  • Journal title : Ocean and Polar Research
  • Volume 32, Issue 3,  2010, pp.247-254
  • Publisher : Korea Institute of Ocean Science & Technology
  • DOI : 10.4217/OPR.2010.32.3.247
 Title & Authors
Identification of Ruditapes philippinarum and Meretrix lusoria Larvae Using Single Cell PCR Analysis and Microscopic Observation
Jung, Seung-Won; Kim, Chang-Soo; Yoo, Jae-Won; Kim, Young-Ok; Lee, Jin-Hwan; Hong, Jae-Sang;
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Single cell PCR analysis and light and scanning electron microscopic techniques were utilized to identify free living bivalve larvae in the coastal waters of Tae-an, on the west coast of Korea. Through DNA sequencing, venerid clam larvae were isolated and identified as Ruditapes philippinarum (99% similarity) and Meretrix lusoria (99%). Under microscopic observation, the D-veliger stage of R. philippinarum exhibited symmetrical shoulder angles and an elliptical ventral form. In contrast, M. lusoria displayed asymmetrical shoulder angles and a round ventral form in the umbonal stage. Size of the R. philippinarum larvae was in length, in height, in width with a length: height ratio of 1.23. Meretrix lusoria was in length, in height, in width with a length: height ratio of 1.25. Experimental results indicate that morphological and molecular characteristics provide evidence for the larval identification of these two venerid clam larvae species in nature.
Single cell PCR;bivalve larvae;Ruditapes philippinarum;Meretrix lusoria;microscopic observation;
 Cited by
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