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Cytochemical Measurement of Lysosomal Responses in the Digestive Cells of Wild Pacific Oyster, Crassostrea gigas on the South Coast of Korea
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  • Journal title : Ocean and Polar Research
  • Volume 34, Issue 2,  2012, pp.151-163
  • Publisher : Korea Institute of Ocean Science & Technology
  • DOI : 10.4217/OPR.2012.34.2.151
 Title & Authors
Cytochemical Measurement of Lysosomal Responses in the Digestive Cells of Wild Pacific Oyster, Crassostrea gigas on the South Coast of Korea
Jeung, Hee-Do; Lee, Jee-Yeon; Hong, Hyun-Ki; Kang, Hyun-Sil; Kim, Young-Ok; Choi, Kwang-Sik;
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Digestive cells of the bivalves have a highly developed lysosomal system and the system is known to be sensitive to changes in environmental qualities. In this study, we measured lysosomal responses of the digestive cells in wild oyster, Crassostrea gigas using frozen section. Oysters were collected in June 2010 from intertidal areas in the inner and outer bay of Gwangyang off the south coast of Korea. From the tissue sections, we measured the digestive cell lysosomal membrane stability (LMS), level of neutral lipids (NL), lipofuscin (LF) and the digestive gland atrophy (DGA). The DGA and condition index of oysters from the inner bay were significantly lower (P<0.05). The statistical test indicated that LMS levels of oysters in the inner bay and the outer bay were not significantly different since a shorter activity was displayed by oysters from the inner bay than that of oysters in outer bay. The LF deposition level of the oysters in the inner bay displayed significantly higher levels than the outer bay (P<0.05). In contrast, the NL accumulation measured from oysters in outer bay was significantly higher than the level observed in the inner bay (P<0.05). Different levels of DGA and LF that were observed in the inner and outer bays were thought to be associated to different level of environmental contamination and these two assays are considered to be good biomarkers.
Crassostrea gigas;digestive gland atrophy;lysosomal membrane stability;neutral lipid;lipofuscin;
 Cited by
산란기 지중해담치 Mytilus galloprovincialis의 조직병리학적 변화 관찰,정희도;이지연;;박경일;강현실;김철원;김형섭;최광식;

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