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Spatial Variation in the Reproductive Effort of Mania Clam Ruditapes philippinarum during Spawning and Effects of the Protozoan Parasite Perkinsus olseni Infection on the Reproductive Effort
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  • Journal title : Ocean and Polar Research
  • Volume 37, Issue 1,  2015, pp.49-59
  • Publisher : Korea Institute of Ocean Science & Technology
  • DOI : 10.4217/OPR.2015.37.1.049
 Title & Authors
Spatial Variation in the Reproductive Effort of Mania Clam Ruditapes philippinarum during Spawning and Effects of the Protozoan Parasite Perkinsus olseni Infection on the Reproductive Effort
Kang, Hyun-Sil; Hong, Hyun-Ki; Yang, Hyun-Sung; Park, Kyung-Il; Lee, Taek-Kyun; Kim, Young-Ok; Choi, Kwang-Sik;
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 Abstract
Spatial variation in the reproductive effort of Manila clam Ruditapes philippinarum is often closely associated with variation in the seawater temperature and food availability, which determines gonad maturity and the quantity of gamates produced during spawning. Previous studies also have reported that severe infection by the protozoan parasite Perkinsus olseni exerts a negative impact on clam reproduction, retarding gonad maturation or decreasing the reproductive effort. In the present study, we investigated impacts of P. olseni infection on the reproductive condition of Manila clam during a spawning season. Histology revealed that 54% of female clams in Wando off the south coast were in spawning, while only 10% of the female from Gomso and 0% of the female from Seonjaedo in Gyeonggi bay off the west coast were engaged in spawning at the end of May in 2004. Ray`s fluid thioglycollate media (RFTM) assay was applied to assess P. olseni infection and indicated that the infection intensity in Wando ( wet tissue) was significantly higher than the levels in Gomso ( wet tissue) and Seonjaedo ( wet tissue, p < 0.001). The size of the ripe female follicle determined from histology was significantly smaller in Wando () compared to the sizes in Gomso () and Seonjaedo (, p < 0.05). Accordingly, the number of ripe eggs in the follicle was significantly fewer among clams in Wando (14) compared to the numbers determined in Gomso (23) and Seonjaedo (22). The absolute quantity of egg in ripe clams from Wando (31.01 mg) was also significantly smaller than Seonjaedo (61.79 mg) and Gomso (133.3 mg). Quantity of total protein, carbohydrate, and lipid in the tissue in the Wando samples was significantly smaller than the quantities determined in Gomso and Seonjaedo (p < 0.001). The observed poor reproductive condition and proximate tissue composition of the females in Wando were, in part, explained by the extremely high level of the parasites, sapping the ability to store energy in the host tissues, which is used in tissue growth and the egg production.
 Keywords
Ruditapes philippinarum;reproductive effort;Perkinsus olseni infection;spatial distribution;
 Language
Korean
 Cited by
 References
1.
Baek MJ, Lee Y-J, Choi K-S, Lee WC, Park HJ, Kwak JH, Kang C-K (2014) Physiological disturbance of the Manila clam Ruditapes philippinarum, by altered environmental conditions in a tidal flat on the west coast of Korea. Mar Pollut Bull 78:137-145 crossref(new window)

2.
Barber BJ, Ford SE, Haskin HH (1988) Effects of the parasite MSX (Haplosporidium nelsoni) on oyster (Crassostrea virginica) energy metabolism. II. Tissue biochemical composition. Comp Biochem Physiol 91A: 603-608

3.
Beninger PG, Lucas A (1984) Seasonal variations in condition, reproductive activity, and gross biochemical composition of two species of adult clam reared in a common habitat: Tapes decussates L. (Jeffreys) and Tapes philippinarum (Adams & Reeve). J Exp Mar Biol Ecol 79:19-37 crossref(new window)

4.
Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911-917 crossref(new window)

5.
Burge CA, Eakin CM, Friedman CS, Froelich B, Hershberger PK, Hofmann EE, Petes LE, Prager KC, Weil E, Willis BL, Ford SE, Harvell CD (2014) Climate change influences on marine infectious diseases: implications for management and society. Annu Rev Mar Sci 6:249-277 crossref(new window)

6.
Casas SM (2002) Estudio de la pekinsosis en la almeja fina, Tapes ducussatus (Linnaeus 1758), de Galicia. Ph. D. Thesis, University of Santiago de Compostela, Spain

7.
Casas SM, Villalba A (2012) Study of perkinsosis in the grooved carpet shell clam Ruditapes decussatus in Galicia (NW Spain). III. The effects of Perkinsus olseni infection on clam reproduction. Aquaculture 356-357:40-47 crossref(new window)

8.
Choi KS, Wilson EA, Lewis DH, Powell EN, Ray SM (1989) The energetic cost of Perkinsus marinus parasitism in oysters: quantification of the thioglycollate method. J Shellfish Res 8:125-131

9.
Choi KS, Powell EN, Lewis DH, Ray SM (1994) Instantaneous reproductive effort in female American oysters, Crassostrea virginica, measured by a new immunoprecipitation assay. Biol Bull 186:41-61 crossref(new window)

10.
Choi KS, Park KI (1997) Report on the occurrence of Perkinsus sp. in the Manila clams, Ruditapes philippinarum in Korea. Korean J Aquacult 10:227-237

11.
Choi KS, Park KI, Cho M, Soudant P (2005) Diagnosis, pathology and taxonomy of Perkinsus sp. isolated from the Manila clam Ruditapes philippinarum in Korea. Korean J Aquacult 18:207-214

12.
Chung EY, Ryou DK, Lee JH (1994) Gonadal development, age and growth of the shortnecked clam, Ruditapes philippinarum (Pelecypoda: Veneidae), on the coast of Kimje, Korea. Korean J Malacol 10:38-54

13.
Chung EY, Hur SB, Hur YB, Lee JS (2001) Gonadal maturation and artificial spawning of the Manila clam, Ruditapes philippinarum (Bivalvia: Veneridae), in Komso Bay, Korea. J Fish Sci Tech 4:208-218

14.
Dang C, de Montaudouin X, Gam M, Paroissin C, Bru N, Caill-Milly N (2010) The Manila clam population in Arcachon Bay (SW France): can it kept sustainable? J Sea Res 63:108-118 crossref(new window)

15.
Dittman DE (1993) The quantitative effects of Perkinsus marinus on reproduction and condition in the Eastern oyster, Crassostrea virginica. J Shellfish Res 12:127

16.
Dittman DE, Ford SE, Padilla DK (2001) Effects of Perkinsus marinus on reproduction and condition of the eastern oyster, Crassostrea virginica, depend on timing. J Shellfish Res 20:1025-1034

17.
Dubois M, Gilles KA, Hamilation JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350-356 crossref(new window)

18.
Harvell CD, Kim K, Burkholder JM, Colwell RR, Epstein PR, Grimes DJ, Hoffmann EE, Lipp EK, Osterhaus ADME, Overstreet RM, Porter JW, Smith GW, Vasta GR (1999) Emerging marine diseases-climate links and anthropogenic factors. Science 285:1505-1510 crossref(new window)

19.
Harvell CD, Mitchell CE, Ward JR, Altizer S, Dobson AP, Ostfeld RS, Samuel MD (2002) Climate warming and disease risks for terrestrial and marine biota. Science 296:2158-2162 crossref(new window)

20.
Holland HJ, Chew KK (1974) Reproductive cycle of the Manila clam Venerupis japonica from Hood Canal, Washington. Proc Natl Shellfish Assoc 64:53-58

21.
Kang C-K, Kang YS, Choy EJ, Kim D-S, Shim B-T, Lee P-Y (2007) Condition, reproductive activity, and gross biochemical composition of the Manila clam, Tapes philippinarum in natural and newly created sandy habitats of the southern coast of Korea. J Shellfish Res 26:401-412 crossref(new window)

22.
Meneghetti F, Moschino V, Da Ros L (2004) Gametogenic cycle and variations in oocyte size of Tapes philippinarum from the Lagoon Venice. Aquaculture 240:473-488 crossref(new window)

23.
Ngo TTT, Choi K-S (2004) Seasonal changes of Perkinsus and Cercaria infections in the Manila clam Ruditapes philippinarum from Jeju, Korea. Aquaculture 239:57-68 crossref(new window)

24.
Park K-I, Choi J-W, Choi K-S (1999) Epizootiology of Perkinsus sp. found in the Manila clam, Ruditapes philippinarum in Komsoe Bay, Korea. J Korean Fish Soc 32:303-309

25.
Park KI, Choi K-S (2001) Spatial distribution of the protozoan parasite Perkinsus sp., found in the Manila clams, Ruditapes philippinarum, in Korea. Aquaculture 203:9-22 crossref(new window)

26.
Park K-I, Choi K-S (2004) Application of enzyme-linked immunosorbent assay for studying of reproduction in the Manila clam Ruditapes philippinarum (Mollusca: Bivalvia): I. Quantifying eggs. Aquaculture 241:667-687

27.
Park K-I, Park JK, Lee J, Choi K-S (2005) Use of molecular markers for species identification of Korean Perkinsus sp. isolated from Manila clam Ruditapes philippinarum. Dis Aquat Org 66:255-263 crossref(new window)

28.
Park K-I, Figueras A, Choi K-S (2006a) Application of enzyme-linked immunosorbent assay for studying of reproduction in the Manila clam Ruditapes philippinarum (Mollusca: Bivalvia): II. Impacts of Perkinsus olseni on clam reproduction. Aquaculture 251:182-191 crossref(new window)

29.
Park K-I, Ngo TTT, Choi S-D, Cho M, Choi K-S (2006b) Occurrence of Perkinsus olseni in the Venus clam Protothaca jedoensis in Korean waters. J Invertbr Pathol 93:81-87 crossref(new window)

30.
Uddin MJ, Yang H-S, Choi K-S, Kim H-J, Hong J-S, Cho M (2010) Seasonal changes in Perkinsus olseni infection and gametogenesis in Manila clam, Ruditapes philippinarum, from Seonjaedo Island in Incheon, off the west coast of Korea. J World Aquacult Soc 41:93-101 crossref(new window)

31.
Villalba A, Reece KS, Ordas C, Casas SM, Figueras A (2004) Perkinsosis in molluscs: a review. Aquat Living Resour 17:411-432 crossref(new window)

32.
Villalba A, Casas SM, Lopez C, Carballal MJ (2005) Study of perkinsosis in the carpet shell clam Tapes decussates in Galicia (NW Spain). II. Temporal pattern of disease dynamics and association with clam mortality. Dis Aquat Org 65:257-267 crossref(new window)

33.
Yamamoto K, Iawata F (1956) Studies on the bivalve Venerupis japonica, in Akkleshi Lake: II. Growth rate and biological minimum size. Bull Hokkaido Reg Fish Res Lab 14:57-63

34.
Yang Y-S, Park K-L, Donaghy L, Adhya M, Choi K-S (2012) Temporal variation of Perkinsus olseni infection intensity in the Manila clam Ruditapes philippinarum in Gomso Bay, off the west coast of Korea. J Shellfish Res 31:685-690 crossref(new window)