Advanced SearchSearch Tips
Effects of Fasting on Brain Expression of Kiss2 and GnRH I and Plasma Levels of Sex Steroid Hormones, in Nile Tilapia Oreochromis niloticus
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Ocean and Polar Research
  • Volume 38, Issue 1,  2016, pp.81-88
  • Publisher : Korea Institute of Ocean Science & Technology
  • DOI : 10.4217/OPR.2016.38.1.081
 Title & Authors
Effects of Fasting on Brain Expression of Kiss2 and GnRH I and Plasma Levels of Sex Steroid Hormones, in Nile Tilapia Oreochromis niloticus
Park, Jin Woo; Kwon, Joon Yeong; Jin, Ye Hwa; Oh, Sung-Yong;
  PDF(new window)
In many fish species, including Nile tilapia (Oreochromis niloticus), gonadal development occurs at the expense of stored energy and nutrients. Therefore, reproductive systems are inhibited by limited food supply. It has been well established that reproductive function is highly sensitive to both metabolic status and energy balance. Nothing is known about the possible mediated connection between energy balance and reproduction. Kisspeptin, a neuropeptide product of the Kiss gene has emerged as an essential gatekeeper of reproduction and may be possibly be linked to energy balance and reproduction in non-mammalians. Thus, in this study, the effect of fasting (10 days) on the expression of kisspeptin and the gonadotropin-releasing hormone (GnRH) gene were assessed in Nile tilapia (male and female) using qRT-PCR. In addition, plasma levels of estradiol- () and 11-ketotestosterone (11-KT) in adult tilapia were measured by ELISA. In male tilapia, fasting reduced Kiss2 and GnRH I mRNA expression in the brain and 11-KT level in comparison with the fed tilapia (p < 0.05). In females, however, there were no significant differences in GnRH I mRNA expression and between fish subjected to fasting and those fed (p > 0.05). These data indicate the impact of nutritional states on kisspeptin as a potential regulatory mechanism for the control of reproduction in male Nile tilapia.
Oreochromis niloticus;Kiss2;GnRH I;sex steroid hormone;fasting;
 Cited by
Biran J, Ben-Dor S, Levavi-Sivan B (2008) Molecular identification and functional characterization of the kisspeptin/kisspeptin receptor system in lower vertebrates. Biol Reprod 79:776-786 crossref(new window)

Bronson FH (1986) Food-restricted, prepubertal, female rats: rapid recovery of luteinizing hormone pulsing with excess food, and full recovery of pubertal development with gonadotropin-releasing hormone. Endocrinology 118:2483-2487 crossref(new window)

Brown RE, Imran SA, Ur E, Wilkinson M (2008) KiSS-1 mRNA in adipose tissue is regulated by sex hormones and food intake. Mol Cell Endocrinol 281:64-72 crossref(new window)

Burcelin R, Thorens B, Glauser M, Gaillard RC, Pralong FP (2003) Gonadotropin-releasing hormone secretion from hypothalamic neurones: stimulation by insulin and potentiation by leptin. Endocrinology 144:4484-4491 crossref(new window)

Cagampang FR, Maeda KI, Tsukamura H, Ohkura S, Ota K (1991) Involvement of ovarian steroids and endogenous opioids in the fasting-induced suppression of pulsatile LH release in ovariectomized rats. J Endocrinol 129: 321-328 crossref(new window)

Castellano JM, Navarro VM, Fernandez-Fernandez R, Nogueiras R, Tovar S, Roa J, Vazquez MJ, Vigo E, Casanueva FF, Aguilar E, Pinilla L, Dieguez C, Tena-Sempere M (2005) Change in hypothalamic KiSS-1 system and restoration of pubertal activation of the reproductive axis by kisspeptin in undernutrition. Endocrinology 146: 3917-3925 crossref(new window)

Cowley MA (2003) Hypothalamic melanocortin neurons integrate signals of energy state. Eur J Pharmacol 480:3-11 crossref(new window)

Dhillo WS, Murphy KG, Bloom SR (2007) The neuroendocrine physiology of kisspeptin in the human. Rev Endocr Metab Dis 8:41-46 crossref(new window)

Felip A, Zanuy S, Pineda R, Pinilla L, Carrillo M, Tena- Sempere M, Gomez A (2009) Evidence for two distinct KiSS genes in non-placental vertebrates that encode kisspeptins with different gonadotropin-releasing activities in fish and mammals. Mol Cell Endocrinol 312:61-71 crossref(new window)

Filby AL, van Aerle R, Duitman J, Tyler CR (2008) The kisspeptin/gonadotropin-releasing hormone pathway and molecular signaling of puberty in fish. Biol Reprod 78:278-289 crossref(new window)

Forbes S, Li XF, Kinsey-Jones J, O'Byrne K (2009) Effects of ghrelin on kisspeptin mRNA expression in the hypothalamic medial preoptic area and pulsatile luteinizing hormone secretion in the female rat. Neurosci Lett 460: 143-147 crossref(new window)

Foster DL, Ebling FJ, Micka AF, Vannerson LA, Bucholtz DC, Wood RI (1989) Metabolic interfaces between growth and reproduction. I. Nutritional modulation of gonadotropin, prolactin, and growth hormone secretion in the growth-limited female lamb. Endocrinology 125:342- 50 crossref(new window)

Grone BP, Carpenter RE, Lee M, Maruska KP, Fernald RD (2012) Food deprivation explains effects of mouthbrooding on ovaries and steroid hormones, but not brain neuropeptide and receptor mRNAs, in an African cichlid fish. Horm Behav 62:18-26 crossref(new window)

Gruenewald DA, Matsumoto AM (1993) Reduced gonadotropinreleasing hormone gene expression with fasting in male rat brain. Endocrinology 132:480-482

Horvath TL, Castaneda T, Tang-Christensen M, Pagotto U, Tschop MH (2003) Ghrelin as a potential anti-obesity target. Curr Pharm Design 9:1383-1395 crossref(new window)

Hoskins LJ, Xu M, Volkoff H (2008) Interactions between gonadotropin-releasing hormone (GnRH) and orexin in the regulation of feeding and reproduction in goldfish (Carassius auratus). Horm Behav 54:379-385 crossref(new window)

Iwasa T, Matsuzaki T, Murakami M, Kinouchi R, Gereltsetseg G, Fujisawa S, Kuwahara A, Yasui T, Irahara M (2010) Sensitivities of mRNA expression levels of Kiss1 and its receptor, Kiss1r, to nutritional status are changed during the developmental period in female rats. J Endocrinol 207:195-202 crossref(new window)

Kaiya H, Kojima M, Hosoda H, Moriyama S, Takahashi A, Kawauchi H, Kangawa K (2003a) Peptide purification, complementary deoxyribonucleic acid (DNA) and genomic DNA cloning, and functional characterization of ghrelin in rainbow trout. Endocrinology 144:5215-5226 crossref(new window)

Kaiya H, Kojima M, Hosoda H, Riley LG, Hirano T, Grau EG, Kangawa K (2003b) Amidated fish ghrelin: purification, cDNA cloning in the Japanese eel and its biological activity. J Endocrinol 176:415-423 crossref(new window)

Kitahashi T, Ogawa S, Parhar IS (2009) Cloning and expression of kiss2 in the zebrafish and medaka. Endocrinology 150:821-831 crossref(new window)

Kohsaka A, Watanobe H, Kakizaki Y, Suda T, Schioth HB (2001) A significant participation of orexin-A, a potent orexigenic peptide, in the preovulatory luteinizing hormone and prolactin surge in the rat. Brain Res 898:166-170 crossref(new window)

Lee JH, Miele ME, Hicks DJ, Phillips KK, Trent JM, Weissman BE, Welch DR (1996) KiSS-1, a novel human malignant melanoma metastasis-suppressor gene. J Natl Cancer I 88:1731-1737 crossref(new window)

Lee YR, Tsunekawa K, Moon MJ, Um HN, Hwang JI, Osugi T, Otaki MN, Sunakawa Y, Kim K, Vaudry H, Kwon HB, Seong JY (2009) Molecular evolution of multiple forms of kisspeptins and GPR54 receptors in vertebrates. Endocrinology 150:2837-2846 crossref(new window)

Li S, Zhang Y, Liu Y, Huang X, Huang W, Lu D, Zhu P, Shi Y, Cheng CHK, Liu X, Lin H (2009) Structural and functional multiplicity of the kisspeptin/GPR54 system in goldfish (Carassius auratus). J Endocrinol 201:407-418 crossref(new window)

Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25:402-408 crossref(new window)

Luque RM, Kineman RD, Tena-Sempere M (2007) Regulation of hypothalamic expression of KiSS-1 and GPR54 genes by metabolic factors: analyses using models and a cell line. Endocrinology 148:4601-4611 crossref(new window)

Mechaly AS, Vinas J, Piferrer F (2011) Gene structure analysis of kisspeptin-2 (Kiss2) in the Senegalese sole (Solea senegalensis): characterization of two splice variants of Kiss2, and novel evidence for metabolic regulation of kisspeptin signaling in non-mammalian species. Mol Cell Endocrinol 339:14-24 crossref(new window)

Messager S, Chatzidaki EE, Ma D, Hendrick AG, Zahn D, Dixon J, Thresher RR, Malinge I, Lomet D, Carlton MB, Colledge WH, Caraty A, Aparicio SA (2005) Kisspeptin directly stimulates gonadotropin-releasing hormone release via G protein-coupled receptor 54. P Natl Acad Sci USA 102:1761-1766 crossref(new window)

Mircea CN, Lujan ME, Pierson RA (2007) Metabolic fuel and clinical implications for female reproduction. J Obstet Gynaecol Can 29:887-902 crossref(new window)

Nakazato M, Murakami N, Date Y, Kojima M, Matsuo H, Kangawa K, Matsukura S (2001) A role for ghrelin in the central regulation of feeding. Nature 409:194-198 crossref(new window)

Nishiguchi R, Azuma M, Yokobori E, Uchiyama M, Matsuda K (2012) Gonadotropin-releasing hormone 2 suppresses food intake in the zebrafish, Danio rerio. Front Endocrinol 3:122

Parhar IS, Ogawa S, Sakuma Y (2004) Laser-captured single digoxigenin-labeled neurons of gonadotropin releasing hormone types reveal a novel G protein-coupled receptor (GPR54) during maturation in cichlid fish. Endocrinology 145:3613-3618 crossref(new window)

Parhar IS, Sato H, Sakuma Y (2003) Ghrelin gene in cichlid fish is modulated by sex and development. Biochem Bioph Res Co 305:169-175 crossref(new window)

Patterson M, Murphy KG, Thompson EL, Patel S, Ghatei MA, Bloom SR (2006) Administration of kisspeptin-54 into discrete regions of the hypothalamus potently increases plasma luteinising hormone and testosterone in male adult rats. J Neuroendocrinol 18:349-354 crossref(new window)

Popa SM, Clifton DK, Steiner RA (2008) The role of kisspeptins and GPR54 in the neuroendocrine regulation of reproduction. Annu Rev Physiol 70:213-238 crossref(new window)

Roa J, Tena-Sempere M (2007) KiSS-1 system and reproduction: comparative aspects and roles in the control of female gonadotropic axis in mammals. Gen Comp Endocr 153:132-140 crossref(new window)

Sakata I, Nakamura K, Yamazaki M, Matsubara M, Hayashi Y, Kangawa K, Sakai T (2002) Ghrelin-producing cells exist as two types of cells, closed- and opened-type cells, in the rat gastrointestinal tract. Peptides 23:531-536 crossref(new window)

Schneider JE (2004) Energy balance and reproduction. Physiol Behav 81:289-317 crossref(new window)

Sisk CL, Bronson FH (1986) Effects of food restriction and restoration on gonadotropin and growth hormone secretion in immature male rats. Biol Reprod 35:554-561 crossref(new window)

Smith C, Wootton RJ (1994) The cost of parental care in Haplochromis argens (Cichlidae). Environ Biol Fish 40:99-104 crossref(new window)

Smith C, Wootton RJ (1995) The costs of parental care in teleost fishes. Rev Fish Biol Fisher 5:7-22 crossref(new window)

Tacon P (1996) Relationships between the expression of maternal behaviour and ovarian development in the mouthbrooding cichlid fish Oreochromis niloticus. Aquaculture 146:261-275 crossref(new window)

Taranger GL, Carrillo M, Schulz RW, Fontaine P, Zanuy S, Felip A, Weltzien F-A, Dufour S, Karlsen O, Norberg B, Andersson E, Hansen T (2010) Control of puberty in farmed fish. Gen Comp Endocr 165:483-515 crossref(new window)

Tuziak SM, Volkoff H (2013) Gonadotrophin-releasing hormone in winter flounder (Pseudopleuronectes americanus): molecular characterization, distribution and effects of fasting. Gen Comp Endocr 184:9-21 crossref(new window)

Unniappan S, Lin X, Cervini L, Rivier J, Kaiya H, Kangawa K, Peter RE (2002) Goldfish ghrelin: molecular characterization of the complementary deoxyribonucleic acid, partial gene structure and evidence for its stimulatory role in food intake. Endocrinology 143:4143-4146 crossref(new window)

Volkoff H, Canosa LF, Unniappan S, Cerda-Reverter JM, Bernier NJ, Kelly SP, Peter RE (2005) Neuropeptides and the control of food intake in fish. Gen Comp Endocr 142:3-19 crossref(new window)

Wren AM, Small CJ, Abbott CR, Dhillo WS, Seal LJ, Cohen MA, Batterham RL, Taheri S, Stanley SA, Ghatei MA, Bloom SR (2001) Ghrelin causes hyperphagia and obesity in rats. Diabetes 50:2540-2547 crossref(new window)