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Effects of Recombinant Aquaporin 3 and Seawater Acclimation on the Expression of Aquaporin 3 and 8 mRNAs in the Parr and Smolt Stages of Rainbow Trout, Oncorhynchus mykiss
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  • Journal title : Ocean and Polar Research
  • Volume 38, Issue 2,  2016, pp.103-113
  • Publisher : Korea Institute of Ocean Science & Technology
  • DOI : 10.4217/OPR.2016.38.2.103
 Title & Authors
Effects of Recombinant Aquaporin 3 and Seawater Acclimation on the Expression of Aquaporin 3 and 8 mRNAs in the Parr and Smolt Stages of Rainbow Trout, Oncorhynchus mykiss
Kim, Na Na; Choi, Young Jae; Lim, Sang-Gu; Kim, Bong-Seok; Choi, Cheol Young;
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This study aimed to examine the role of two aquaporin isoforms (AQP3 and AQP8) in response to the hyperosmotic challenge of transitioning from freshwater (FW) to seawater (SW) during parr and smoltification (smolt) using the rainbow trout, Oncorhynchus mykiss. We examined the changes in the expression of AQPs mRNAs in the gills and intestine of the parr and smolt stages of rainbow trout transferred from FW to SW using quantitative real-time PCR in an osmotically changing environment [FW, SW, and recombinant AQP3 (rAQP3) injection at two dosage rates]. Correspondingly, AQPs were greater during smoltification than during parr stages in the rainbow trout. Plasma osmolality and gill -ATPase activity increased when the fish were exposed to SW, but these parameters decreased when the fish were exposed to SW following treatment with rAQP3 during the transition to seawater. Our results suggest that AQPs play an important role in water absorbing mechanisms associated with multiple AQP isoforms in a hyperosmotic environment.
osmoregulation;recombinant aquaporin 3;rainbow trout;parr;smolt;
 Cited by
Ackerman PA, Forsyth RB, Mazur CF, Iwama GK (2000) Stress hormones and the cellular stress response in salmonids. Fish Physiol Biochem 23:327-336 crossref(new window)

Borgnia M, Nielsen S, Engel A, Agre P (1999) Cellular and molecular biology of the aquaporin water channels. Annu Rev Biochem 68:425-458 crossref(new window)

Choi YJ, Shin HS, Kim NN, Shin SH, Yamamoto Y, Ueda H, Lee J, Choi CY (2013) Expression of aquaporin-3 and -8 mRNAs in the parr and smolt stages of sockeye salmon, Oncorhynchus nerka: Effects of cortisol treatment and seawater acclimation. Comp Biochem Physiol A 165:228-236 crossref(new window)

Choi YJ, Kim NN, Choi CY (2015) Profiles of hypothalamuspituitary-interrenal axis gene expression in the parr and smolt stages of rainbow trout, Oncorhynchus mykiss:Effects of recombinant aquaporin 3 and seawater acclimation. Comp Biochem Phys A 182:14-21

Evans DH (1993) Osmotic and ionic regulation. In: Evans DH (ed) The Physiology of fishes. CRC Press, Boca Raton, Florida, pp 315-341

Finn RN, Cerda J (2011) Aquaporin evolution in fishes. Front Physiol 2:44

Ishibashi K, Hara S, Kondo S (2009) Aquaporin water channels in mammals. Clin Exp Nephrol 13:107-117 crossref(new window)

Ishibashi K, Kondo S, Hara S, Morishita Y (2011) The evolutionary aspects of aquaporin family. Am J Physiol 300:R566-R576

Kim YK, Lee SY, Kim BS, Kim DS, Nam YK (2014) Isolation and mRNA expression analysis of aquaporin isoforms in marine medaka Oryzias dancena, a euryhaline teleost. Comp Biochem Phys A 171:1-8

Marshall W (2003) Rapid regulation of NaCl secretion by estuarine teleost fish: coping strategies for short-duration freshwater exposures. Biochim Biophys Acta 1618:95-105 crossref(new window)

Marshall WS, Cozzi RR, Pelis RM, McCormick SD (2005) Cortisol receptor blockade and seawater adaptation in the euryhaline teleost Fundulus heteroclitus. J Exp Zool Part A 303:132-142

Marshall WS, Grosell M (2006) Ion transport, osmoregulation and acid-base balance. In: Evans DH, Claiborne JB (eds) The Physiology of Fishes, 3rd ed. CRC Press, Boca Raton, Florida, pp 177-230

Martinez A-S, Cutler CP, Wilson G, Phillips C, Hazon N, Cramb G (2005) Cloning and expression of three aquaporin homologues from the European eel (Anguilla anguilla): effects of seawater acclimation and cortisol treatment on renal expression. Biol Cell 97:615-627 crossref(new window)

Matsuzaki T, Tajika Y, Tserentsoodol N, Suzuki T, Aoki T, Hagiwara H, Takata K (2002) Aquaporins: a water channel family. Anat Sci Int 77:85-93 crossref(new window)

McCormick SD (2001) Endocrine control of osmoregulation in teleost fish. Am Zool 41:781-794

McCormick SD, Bern HA (1989) In vitro stimulation of $Na^+-K^+$-ATPase activity and oubain binding by cortisol in coho salmon gill. Am J Physiol 256:R707-R715

McCormick SD, Saunders RL (1987) Preparatory physiological adaptations for marine life of salmonids: osmoregulation, growth, and metabolism. Am Fish S S 1:211-229

Park MS, Kim NN, Shin HS, Min BH, Kil G-S, Cho SH, Choi CY (2012) Hypoosmotic shock adaptation by prolactin involves upregulation of arginine vasotocin and osmotic stress transcription factor 1 mRNA in the cinnamon clownfish Amphiprion melanopus. Anim Cells Syst 16:391-399 crossref(new window)

Sakamoto T, McCormick SD (2006) Prolactin and growth hormone in fish osmoregulation. Gen Comp Endocr 147:24-30 crossref(new window)

Singer TD, Finstad B, McCormick SD, Wiseman SD, Schulte PM, McKinley RS (2003) Interactive effects of cortisol treatment and ambient seawater challenge on gill $Na^+-K^+$-ATPase and CFTR expression in two strains of Atlantic salmon smolts. Aquaculture 222:15-28 crossref(new window)

Tingaud-Sequeira A, Calusinska M, Finn RN, Chauvigne F, Lozano J, Cerda J (2010) The zebrafish genome encodes the largest vertebrate repertoire of functional aquaporins with dual paralogy and substrate specificities similar to mammals. BMC Evol Biol 10:38 crossref(new window)

Tipsmark CK, Sorensen KJ, Madsen SS (2010) Aquaporin expression dynamics in osmoregulatory tissues of Atlantic salmon during smoltification and seawater acclimation. J Exp Biol 213:368-379 crossref(new window)

Ueda H (2011) Physiological mechanism of homing migration in Pacific salmon from behavioral to molecular biological approaches. Gen Comp Endocr 170:222-232 crossref(new window)

Uchida K, Kaneko T, Yamauchi K, Hirano T (1996) Morphometrical analysis chloride cell activity in the gill filaments and lamellae and changes in $Na^+-K^+$-ATPase activity during seawater adaptation in chum salmon fry. J Exp Zool 276:193-200 crossref(new window)

Uchida K, Kaneko T, Miyazaki H, Hasegawa S, Hirano T (2000) Excellent salinity tolerance of mozambique tilapia (Oreochromis mossambicus): elevated chloride cell activity in the branchial and opercular epithelia of the fish adapted to concentrated seawater. Zool Sci 17:149-160 crossref(new window)

Zardoya R (2005) Phylogeny and evolution of the major intrinsic protein family. Biol Cell 97:397-414 crossref(new window)