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Improvement of SNPs detection efficient by reuse of sequences in Genotyping By Sequencing technology
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 Title & Authors
Improvement of SNPs detection efficient by reuse of sequences in Genotyping By Sequencing technology
Baek, Jeong-Ho; Kim, Do-Wan; Kim, Junah; Lee, Tae-Ho;
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Recently, the most popular technique to determine the Genotype, genetic features of individual organisms, is the GBS based on SNP from sequences determined by NGS. As analyzing the sequences by the GBS, TASSEL is the most used program to identify the genotypes. But, TASSEL has limitation that it uses only the partial sequences that is obtained by NGS. We tried to improve the efficiency in use of the sequences in order to solve the limitation. So, we constructed new data sets by quality checking, filtering the unused sequences with error rate below 0.1% and clipping the sequences considering the location of barcode and enzyme. As a result, approximately over 17% of the SNP detection efficiency was increased. In this paper, we suggest the method and the applied programs in order to detect more SNPs by using the disused sequences.
Genome;Reuse;Genotyping By Sequencing;SNP;Efficiency;
 Cited by
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