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Development and Characterization of Chloroplast Simple Sequence Repeat markers in Pinus koraiensis
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  • Journal title : Journal of Korean Forest Society
  • Volume 104, Issue 4,  2015, pp.549-557
  • Publisher : Korean Forest Society
  • DOI : 10.14578/jkfs.2015.104.4.549
 Title & Authors
Development and Characterization of Chloroplast Simple Sequence Repeat markers in Pinus koraiensis
Lee, Jei-Wan; Baek, Seung-Hoon; Hong, Kyung-Nak; Hong, Yong-Pyo; Lee, Seok-Woo; Ahn, Ji-Young;
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 Abstract
Novel cpSSR primers were developed based on the sequence information of the Pinus koraiensis chloroplast genome. A total of 30 cpSSR loci were detected in the chloroplast genome, and a total of 30 primer sets flanking those loci were designed. All primer sets were successfully amplified for chloroplast DNA in P. koraiensis. The cross-species transferability of the 30 primer sets was considerably high in P. pumila (100%) and P. paviflora (97%) belonging to the same Subgenus (Strobus) of P. koraiensis. Meanwhile, the transferability was relatively low (73%) in P. densiflora and P. sylvestris belonging to Subgenus Pinus. A total of 13 cpSSR loci out of the 30 loci were polymorphic in the Mt. Jumbong population of P. koraiensis. The mean of haploid diversity(H) was 0.512. The number of haplotypes(N) and the haplotype diversity() were 25 and 0.992, respectively. Of the 25 haplotypes, 22 were unique in the analyzed population. The unique haplotypes differentiated 22 individuals (79%) from the total of 28 individuals. In conclusion, the novel cpSSR primers developed in this study would be applicable to other Pinus species, especially the subgenus Strobus, and provide a high level of polymorphism for the study of genetic variation of P. koraiensis.
 Keywords
Pinus koraiensis;SSR;microsatellite;chloroplast DNA;genetic diversity;transferability;
 Language
Korean
 Cited by
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