JOURNAL BROWSE
Search
Advanced SearchSearch Tips
The Endocrine Disruption Induced by Ampicillin and Amoxicillin in Japanese Medaka (Oryzias latipes)
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
The Endocrine Disruption Induced by Ampicillin and Amoxicillin in Japanese Medaka (Oryzias latipes)
Kim, Kyung-Tae; Kim, Pan-Gyi;
  PDF(new window)
 Abstract
The study was designed to determine the estrogenic effect of some penicillins on endocrine function in adult Japanese medaka (Oryzias latipes). Vitellogenin (Vtg) produced in male fish has been used for a biomarker to study endocrine disrupters. was used a positive control that was induced Vtg in male fish. Result of total protein qantification and ELISA for female and male fish were exposed to 10ng/ml for days. As a result, male fish exposed to amoxicillin respectively appeared 0.75, 0.23, 8.21 and of 1, 10, 100 and 1000 ppm respectively, that value was elevated compared with control male fish. Male fish exposed to ampicillin respectively appeared 1.85, 4.68, 0.85 and of 1, 10, 100 and 1000 ppm respectively, that value was elevated compared with control male fish. This study is one of the first reports suggesting potential endocrine disruption of some penicillins in aquatic ecosystem. These results suggest that vitellogenin and estrogen receptor induction patterns alter in male medaka treated with selected estrogenic compounds, and that these results may be useful molecular biomarkers for screening estrogenic EDCs (endocrine-disrupting chemicals) in the shortest possible time.
 Keywords
penicillin;ampicillin;amoxicillin;endocrine disruption;vitellogenin;Japanese medaka (Oryzias latipes);ELISA;Western blot;
 Language
English
 Cited by
 References
1.
Boxall, A.B.A. : The environmental side effects of medication: How are human and veterinary medicines in soils and water bodies affecting human and environmental health. European Molecular Biology Organization reports, 5, 1110-1116, 2004a crossref(new window)

2.
Boxall, A.B.A., Fogg, L.A., Blackwell, P.A., Kay, P., Pemberton, E.J. and Croxford, A. : Veterinary medicines in the environment. Review of Environmental Contamination and Toxicology, 180, 1-91, 2004b crossref(new window)

3.
Kang, H.J., Choi, K.H., Kim, M.Y. and Kim, P.G. : Endocrine disruption induced by some sulfa drugs and tetracyclines on Oryzias latipes. Korean Journal of Environmental Health, 32(3), 227-234, 2006

4.
Kang, H.J., Kim, H.S., Choi, K.H., Kim, K.T. and Kim, P.G. : Several human pharmaceutical residues in aquatic environment may result in endocrine disruption in Japanese Medaka (Oryzias latipes). Korean Journal of Environmental Health, 31(3), 227-233, 2005

5.
Kazuto Nishi, Miyuki Chikae, Yasuhiko Hatano, Haruki Mizukami, Michiaki Yamashita, Ryuzo Sakakibara and Eiichi Tamiya : Development and application of a monoclonal antibody-based sandwich ELISA for quantification of Japanese medaka (Oryzias latipes) vitellogenin. Comparative Biochemistry and Physiology Part C, 132, 161-169, 2002 crossref(new window)

6.
Lullmann, H., Mohr, K., Ziegler, A. and Bieger, D. : Color atlas of pharmacology. 266-281, Thieme 2nd ed. New York, 2000

7.
Yamaguchi Akemi, Hiroshi Ishibashi, Shinya Kohra, Koji Arizono and Nobuaki Tominaga : Short-term effects of endocrine-disrupting chemicals on the expression of estrogen-responsive genes in male medaka (Oryzias latipes). Aquatic Toxicology, 72, 239-249, 2005 crossref(new window)