JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Heme Oxygenase-1 (HO-1) Induction by UVB (290-320nm) Radiation in ICR Mice
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Heme Oxygenase-1 (HO-1) Induction by UVB (290-320nm) Radiation in ICR Mice
Choi, Wook-Hee; Kim, Tae-Hwan; Ahn, Ryoung-Me;
  PDF(new window)
 Abstract
The induction of heme oxygenase-1(HO-1) by UV radiation provides a protective defence against oxidative stress, and has been well demonstrated in skin irradiated with UVA, but not UVB. In this study, we show that the induction of cutaneous HO-l can be attributed to UVB radiation. The expression of HO-1 mRNA was assessed in vivo by reverse transcription-polymerase chain reaction (RT-PCR) analysis, and HO-1 enzyme activity was measured in microsomal preparation from irradiated mice. The mRNA level of HO-1 increases in liver and skin from 1d to 3d after UVB exposure. The results of gene expression were same pattern of HO-1 enzyme activity in skin, but not in liver. HO-1 mRNA in liver resulted in a progressive increase to 4d after UVB exposure, but HO-1 activity in liver increased to 2d. This finding indicates that UVB radiation is an important inducer of HO-1 and increases in HO activity may protect tissue directly or indirectly from oxidative stress.
 Keywords
UVB radiation;Heme Oxygenase-1;mouse skin & liver;
 Language
Korean
 Cited by
 References
1.
Allanson, M. and Reeve, V. E. : Immunoprotective UVA(320-400 nm) irradiation upregulates Heme Oxygenase-1 in the dermis and epidermis of hairless mouse skin. The Journal of Investigative Dermatology, 122, 1030-1036, 2004 crossref(new window)

2.
Yachie, A. : Heme oxygenase and its role in defense system; paradigm shift of anti-inflammatory therapy. Japanese Journal of Clinical Immunology, 30(1), 11- 21, 2007 crossref(new window)

3.
Hanselmann, C., Mauch, C. and Werner, S. : Haem oxygenase-1: a novel player in cutaneous wound repair and psoriasis? The Biochemical Journal, 353, 459-466, 2001 crossref(new window)

4.
Kikuchi, G., Yoshida, T. and Noguchi, M. : Heme oxygenase and heme degradation. Biochemical and Biophysical Research Communications, 338(1), 558- 567, 2005 crossref(new window)

5.
Cooper, K. L., Liu, K. J. and Hudson, L. G. : Contributions of reactive oxygen species and mitogenactivated protein kinase signaling in arsenite-stimulated hemeoxygenase-1 production. Toxicology and Applied Pharmacology, 218(2), 119-127, 2007 crossref(new window)

6.
Tyrrell, R. M. and Reeve, V. E. : Potential protection of skin by acute UVA irradiation-from cellular to animal models. Progress in Biophysics and Molecular Biology, 92(1), 86-91, 2006

7.
Dong, Z., Lavrovsky, Y., Venkatachalam, M. A. and Roy, A. K. : Heme Oxygenase-1 in tissue pathology. American Journal of Pathology, 156(5), 1485-1488, 2000 crossref(new window)

8.
Lee, M. K., Yi, S. Y., Ann, H. S. and Ahn, R. M. : Lipid Peroxidation and Antioxidase Activities on hairless mouse induced by UVB irradiation and effects of tannic acid. Korean Journal of Environmental Health, 22(4), 102-108, 1996

9.
Obermüller-Jevic, U. C., Schlegel, B., Flaccus, A. and Biesalski, H. K. : The effect of beta-carotene on the expression of interleukin-6 and heme oxygenase-1 in UV-irradiated human skin fibroblasts in vitro. FEBS Letters, 509(2), 186-190, 2001 crossref(new window)

10.
Llesuy, S . F. and T omaro, M. L. : H eme oxygenase and oxidative stress. Evidence of involvement of bilirubin as physiological protector against oxidative damage. Biochimica et Biophysica Acta, 1223(1), 9- 14, 1994 crossref(new window)

11.
Yamaguchi, T., Terakado, M., Horio, F., Aoki, K., Nakajima, M. and Nakajima, H. : Role of bilirubin as an antioxidant in an ischemia-reperfusion of rat liver and induction of heme oxygenase. Biochemical and Biophysical Research Communications, 223(1), 129- 135, 1996 crossref(new window)

12.
Ossola, J. O. and Tomaro, M. L. : Heme oxygenase induction by UVA radiation. A response to oxidative stress in rat liver. The International Journal of Biochemistry & Cell Biology, 30(2), 285-292, 1998 crossref(new window)

13.
Choi, W. H., Ahn, R. M., Do, S. H., Jeong, D. H., Ryu, S. Y., Kwak, D. M., Kwon, O. D., Jeong, K. S. and Kim, T. H. : Changes of lipid peroxide and antioxidant enzymes by UVB-induced oxidative stress. Laboratory Animal Research, 22(3), 227-232, 2006

14.
Ahn, R. M. : Effects of natural extracts on COX-1 and COX-2 mRNA expression on UVB-induced skin inflammation in C57BL/6 mouse. Korean Journal of Environmental Health, 32(6), 566-570, 2006

15.
Lincoln, B. C., Healey, J. F. and Bonkovsky, H. L. : Regulation of hepatic haem metabolism. Disparate mechanisms of induction of haem oxygenase by drugs and metals. The Biochemical Journal, 250(1), 189-196, 1988

16.
Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. : Protein measurement with the Folin phenol reagent. The Journal of Biological Chemistry, 193(1), 265-275, 1951

17.
Basu-Modak, S., Lüscher, P. and Tyrrell, R. M. : Lipid metabolite involvement in the activation of the human heme oxygenase-1 gene. Free Radical Biology & Medicine, 20(7), 887-897, 1996 crossref(new window)

18.
Reeve, V. E and Tyrrell, R. M. : Heme oxygenase induction mediates the photoimmunoprotective activity of UVA radiation in the mouse. Proceedings of the National Academy of Sciences of the United States of America, 96(16), 9317-9321, 1999

19.
Applegate, L. A., Noël, A., Vile, G., Frenk, E. and Tyrrell, R. M. : Two genes contribute to different extents to the heme oxygenase enzyme activity measured in cultured human skin fibroblasts and keratinocytes: Implications for protection against oxidant stress. Photochemistry and Photobiology, 61(3), 285- 291, 1995 crossref(new window)

20.
Tyrrell, R. M. : Activation of mammalian gene expression by the UV component of sunlight from models to reality. BioEssays, 18(2), 139-148, 1996 crossref(new window)

21.
Vile, G. F., Basu-Modak, S., Waltner, C. and Tyrrell. R. M. : Heme oxygenase 1 mediates an adaptive response to oxidative stress in human skin fibroblasts. Proceedings of the National Academy of Sciences of the United States of America, 91(7), 2607- 2610, 1994

22.
Yasuko, S. and Takashi, H. : Time course of changes in antioxidant enzymes in human skin fibroblasts after UVA irradiation. Journal of Dermatological Science, 14, 225-232, 1997 crossref(new window)

23.
Choi, W. H., Ann, H. S. and Ahn, R. M. : Prevention of ultraviolet B radiation(280-320 nm) induced contact hypersensitivity by EGb 761. Korean Journal of Environmental Health, 31(1), 7-14, 2005

24.
Allanson, M. and Reeve, V. E. : Ultraviolet A (320- 400 nm) modulation of ultraviolet B (290-320 nm)- induced immune suppression is mediated by carbon monoxide. The Journal of Investigative Dermatology, 124(3), 644-650, 2005 crossref(new window)

25.
Quan, S., Kaminski, P. M., Yang, L., Morita, T., Inaba, M., Ikehara, S., Goodman, A. I., Wolin, M. S. and Abraham, N. G. : Heme oxygenase-1 prevents superoxide anion-associated endothelial cell sloughing in diabetic rats. Biochemical and Biophysical Research Communications, 315(2), 509-516, 2004 crossref(new window)

26.
Fang, J ., Nakamura, H. and Iyer, A. K. : T umor-targeted induction of oxystress for cancer therapy. Journal of Drug Targeting, 15(7-8), 475-486, 2007 crossref(new window)

27.
Khelifi, A. F., Prise, V. E. and Tozer, G. M. : Effects of tin-protoporphyrin IX on blood flow in a rat tumor model. Experimental Biology and Medicine, 228(5), 481-485, 2003

28.
Jozkowicz, A., Was, H. and Dulak, J. : Heme Oxygenase- 1 in Tumors: Is It a False Friend? Antioxidants & Redox Signaling, 9(12), 1-19, 2007 crossref(new window)