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Effect of Exposure Concentration and Time of Fuel Additives on the Indigenous Microbial Community in Forests
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 Title & Authors
Effect of Exposure Concentration and Time of Fuel Additives on the Indigenous Microbial Community in Forests
Cho, Won-Sil; Cho, Kyung-Suk;
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The toxicity of methyl tert-butyl ether (MTBE), tert-butyl alcohol (TBA) and formaldehyde (FA) on the indigenous microbial community in forest soil was studied. MTBE, TBA and FA with different concentrations were added into microcosms containing forest soil samples. After 10 and 30 days, total viable cell number and dehydrogenase activity in the microcosms were evaluated. Bacterial communities in the microcosms were also analyzed using a denaturing gradient gel electrophoresis (DGGE). Dehydrogenase activity and total viable cell number were decreased according to the increase of MTBE, TBA and FA concentrations (P<0.05). FA toxicity was the highest, but TBA toxicity was the lowest. The results of principal component analysis using DGGE fingerprints showed that the microbial communities contaminated MTBE, TBA and FA were grouped by exposure time not exposure concentration. Dominant species in the microcosms were as follows: Photobacterium damselae sub sp. and Bacillus sp. KAR28 for MTBE; Mycobacterium sp. and Uncultured Clostridium sp. for TBA; and Uncultured Paenibacillaceae bacterium and Anxynobacillus, Flavithermus for FA.
Fuel additives;forest soil;methyl tert-butyl ether (MTBE);tert-butyl alcohol (TBA) and formaldehyde (FA);microbial activity and structure;
 Cited by
유류오염토양이 식물식생에 미치는 영향에 관한 연구,최민주;김주영;김정훈;최상일;

한국지하수토양환경학회지:지하수토양환경, 2010. vol.15. 1, pp.50-56
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