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Prevalence and Identification of Cryptosporidium spp. from Swine Slurry
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 Title & Authors
Prevalence and Identification of Cryptosporidium spp. from Swine Slurry
Chun, Kae-Shik;
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Cryptosporidium spp. were detected in 17 of 135 swine lagoon samples from five farms by 18S ribosomal DNA locus and PCR. Seventeen positive samples identified were included two distinctive genotypes C. suis and Cryptosporidium sp. based on a small-subunit rRNA gene-based PCR-restriction fragment length polymorphism analysis. Cryptosporidium spp. were detected out of farrowing, farrowing and nursery (mix), and finishing. Prevalence rate was 12.6% with infection rates between 3.7 and 18.5%. We concluded that Cryptosporidium oocysts can persist in treated lagoon and potentially contaminate surface water through improper discharge. This study was undertaken for the evaluation of the infection status of the genotypes of Cryptosporidium spp. in swine lagoon.
Cryptosporidium;prevalence rate;C. suis;Cryptosporidium sp.;swine slurry;
 Cited by
Tyzzer, E. : A sporozoon found in the peptic glands of the common mouse. Proceedings of the Society for Experimental Biology Medicine, 5, 12-13, 1907

Xiao, L., Fayer, R., Ryan, U., Upton, S. J. : Cryptosporidium taxonomy: recent advances and implications for public health. Clinical Microbiology Reviews, 17(1), 72-97, 2004 crossref(new window)

Kennedy, G. A., Kreitner, G. L., Strafuss, A. C. Cryptosporidiosis in three pigs. Journal of the American Veterinary Medical Association, 170, 348-350, 1977

Izumiyama, S., Furukawa, I., Kuroki, T., Yamai, S., Sugiyama, H., Yagita, K., Endo, T. : Prevalence of C. parvum infections in weaned piglets and fattening porkers in Kanagawa Prefecture. Japan, Revista da Sociedade Brasileira de Medicina Tropical, 54, 23-26, 2001

Hamnes, I. S., Gjerde, B. K., Forberk, T., Robertson, L. J. : Occurrence of Cryptosporidium and Giardia in suckling piglets in Norway. Veterinary Parasitology, 141, 30-41, 2006 crossref(new window)

Langkjaer, R. B., Vigre, H., Enemark, H. L., Maddox-Hyttel, C. : Molecular and phylogenetic characterization of Cryptosporidium and Giardia from pigs and cattle in Denmark. Parasitology, 134, 339-350, 2007 crossref(new window)

Ayeni, A. O., Olubunmi, P. A., Abe, J. O. : The occurrence and effect of Cryptosporidium species on livestock in Ile-Ife, Nigeria. Tropical Veterinarian, 3, 96-100, 1985

Enemark, H. L., Bille-Hansen, V., Lind, P., Heegaard, P. M., Vigre, H., Ahrens, P., Thamsborg, S. M. : Pathogenicity of Cryptosporidium parvum - evalution of an animal infection model. Veterinary Parasitology, 113, 35-57, 2003 crossref(new window)

Ryan, U. M., Samarasinghe, B., Read, C., Buddie, J. R., Robertson, I. D., Thompson, R. C. A. : Identification of a novel Cryptosporidium genotype in pigs. Applied and Environmental Microbiology, 69, 3970-3974, 2003 crossref(new window)

Plutzer, J., Karanis, P. : Genotype and subtype analyses of Cryptosporidium isolates from cattle in Hungary. Veterinary Parasitology, 146, 357-362, 2007 crossref(new window)

Johnson, D. W., Pieniazek, N. J., Griffin, D. W., Misener, L., Rose, J. B. : Develpoment of a PCR protocol for sensitive detection of Cryptosporidium oocysts in water samples. Applied and Environmental Microbiology, 61, 3849-3855, 1995

Chung, K. S. : The genetic correlations among serotypes and PFGE patterns of streptococcus pneumoniae isolated in Korea. Journal of Environmental Health Sciences, 30, 15-21, 2004

Rucker, N. J., Braithwaite, S. L., Topp, E., Edge, T., Lapen, D. R., Wilkes, G, Robertson, W., Medeires, D., Sensen, C. W., Neumann, N. F. : Tracking host sources of Cryptosporidium spp. in raw water for improved health risk assessment. Applied and Environmental Microbiology, 72, 3945-3957, 2007 crossref(new window)

Xiao, L., Moore, J. E., Ukoh, U., Gatei, W., Lowery, C. J., Murphy, T. M., Dooley, J. S., Millar, B. C., Rooney, P. J., Rao, J. R. : Prevalence and identity of Cryptosporidium spp. in pig slurry. Applied and Environmental Microbiology, 72, 4461-4463, 2006 crossref(new window)

Straw, B. E., Zimmermann, J. J., D'Aliaire, W. L., Taylor, D. J. : Diseases of swine, 9th edition. Blackwell publishing, 2006

Upton, S. J., Current, W. L. : The species of Cryptosporidium (Apicomplexa : Cryptosporididae) infecting mammals. The Journal of Parasitology, 71, 625-629, 1985 crossref(new window)

Morgan, U. M., Buddle, R., Armson, A., Elliot, A., Thompson, R. C. A. : Molecular and biological characterization of Cryptosporidium in pigs. Australian Veterinary Journal, 77, 44-47, 1999 crossref(new window)

Xiao, L., Herd, R. P., Bowman, G L. : Prevalence of Cryptosporidium and Giardia infections on two Ohio pig farms with different management systems. Veterinary Parasitology, 52, 331-336, 1994 crossref(new window)

Sanford, S. E. : Enteric cryptosporidial infection in pigs, 184 cases (1981-1985). Journal of the American Veterinary Medical Association, 190, 695-698, 1987

Maddox-Hyttel, R. B., Langkjaer, H. L., Vigre, H. : Cryptosporidium and Giardia in different age groups of Danish cattle and pigs-occurrence and management associated risk factors. Veterinary Parasitology, 141, 48-59, 2006 crossref(new window)

Olsen, M. E., Thorlakson, C. L., Deselliers, L., Morck, D. W., McAllister, T. A. : Giardia and Cryptosporidium in Canadian farm animals. Veterinary Parasitology, 68, 75-381, 1997 crossref(new window)

Fayer, R., Dubey, J. P., Lindsay, D. S. : Zoonotic protozoa: from land to sea. Trends in Parasitology, 20, 531-536, 2004 crossref(new window)