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Growth Optimization of Delftia sp. for the Odor Control of Organic Waste
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 Title & Authors
Growth Optimization of Delftia sp. for the Odor Control of Organic Waste
Kwon, Hyuk-Ku; Jung, Joon-Oh; Chu, Duk-Sung; Lee, Jang-Hoon;
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 Abstract
We isolated and identified a microorganism which was excellent for ammonia oxidation in the biological control of ammonia gas in odor producing materials from organic composting. The isolated strain was tested for growth characteristics and ammonia elimination efficiency under various conditions of temperature, pH, carbon concentration and ammonia concentration. The strain was isolated from a culture broth used in a producing test with Griess-Ilosvay reagent. The results of 16S rRNA sequence from the isolated strain by using BLANST (Basic Local Alignment Search Tool) and confirming RDP (Ribosomal Database Project II) and ERRD (The European Ribosomal RNA Database) indicate that the strain is related to Delftia sp. UV-Spectrophotometer (Shimadzu, UVmini-1240) was used as a microbial growth test by measuring turbidity on OD660nm and ammonia concentration was measured by Spectrophotometer (HACH, DR-4000). The optimum growth culture conditions of the ammonia oxidizer Delftia sp. were , pH 7, glucose concentration 1.00% and 0.5 g/l. Ammonia elimination efficiency was over 94% under the same conditions.
 Keywords
microorganism;ammonia oxidation;16S rRNA;Delftia sp.;culture condition;
 Language
Korean
 Cited by
1.
적환장에서 발생하는 악취 분포 특성에 관한 연구,전재식;김은숙;유승성;오석률;최한영;

한국환경보건학회지, 2013. vol.39. 5, pp.418-425 crossref(new window)
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