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The Whitening Effect of Angelicae dahuricae Radix Water Extract in Brown Guinea Pigs
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 Title & Authors
The Whitening Effect of Angelicae dahuricae Radix Water Extract in Brown Guinea Pigs
Kim, Pil-Sun; Kim, Young-Chul;
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 Abstract
The purpose of this study was to investigate the whitening effect of Angelicae dahuricae Radix water extract (ADWE) by its application on artificial tanning spots which were induced by 1,500 of UVB radiation on the backs of brown guinea pigs weighing approximately 450~500 g. Thirty of ADWE, at each application, were applied twice a day, 5 days a week, for 8 weeks to the guinea pigs. The artificial tanning spots were divided into 3 groups which were vehicle control group [propylene glycol: ethanol: water (5:3:2)], positive control group (2% hydroquinone) and experimental group (2% ADWE). The visible whitening effect and changes in melanin index were evaluated once a week. On completion of the experiment, the animals were sacrificed under anesthetization, and the artificial tanning spots were excised by biopsy punch and stained with Hematoxylin and Eosin (H&E) to observe histological change and also stained with Fontana-Masson's silver (F-M) and S-100 to observe melanin pigmentation and melanocytes. In the gross observation, the experimental group showed higher pigmentation than the positive control group but lower pigmentation than the vehicle control group. Histological observation confirmed that ADWE had a positive whitening effect by showing a lower distribution of melanin and melanocytes in the epidermis of experimental group than in the vehicle control group.
 Keywords
Angelicae dahuricae Radix;brown guinea pig;melanin;whitening effect;
 Language
Korean
 Cited by
1.
백지 에탄올추출물의 미백효능 연구,김필순;

한국산학기술학회논문지, 2011. vol.12. 9, pp.4038-4045 crossref(new window)
1.
Whitening Effects of Angelica dahurica Radix Ethanol Extract, Journal of the Korea Academia-Industrial cooperation Society, 2011, 12, 9, 4038  crossref(new windwow)
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