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Dechlorination of High Concentrations of Tetrachloroethylene Using a Fixed-bed Reactor
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 Title & Authors
Dechlorination of High Concentrations of Tetrachloroethylene Using a Fixed-bed Reactor
Chang, Young-C.; Park, Chan-Koo; Jung, Kweon; Kikuchi, Shintaro;
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We evaluated the properties of a fixed-bed column reactor for high-concentration tetrachloroethylene (PCE) removal. The anaerobic bacterium Clostridium bifermentans DPH-1 was able to dechlorinate PCE to cis-1,2-dichloroethylene (cDCE) via trichloroethylene (TCE) at high rates in the monoculture biofilm of an upflow fixed-bed column reactor. The first-order reaction rate of C. bifermentans DPH-1 was relatively high at , and comparable to rates obtained by others. When we gradually raised the influent PCE concentration from to , the degree of PCE dechlorination rose to over 99% during the operation period of 2,000 h. In order to maintain efficiency of transformation of PCE in this reactor system, more than 6 h hydraulic retention time (HRT) is required. The maximum volumetric dechlorination rate of PCE was determined to be of reactor , which is relatively high compared to rates reported previously. The results of this study indicate that the PCE removal performance of this fixed-bed reactor immobilized mono-culture is comparable to that of a fixed-bed reactor mixture culture system. Furthermore, our system has the major advantage of a rapid (5 days) start-up time for the reactor. The flow characteristics of this reactor are intermediate between those of the plug-flow and complete-mix systems. Biotransformation of PCE into innocuous compounds is desirable; however, unfortunately cDCE, which is itself toxic, was the main product of PCE dechlorination in this reactor system. In order to establish a system for complete detoxification of PCE, co-immobilization of C. bifermentans DPH-1 with other bacteria that degrade cDCE aerobically or anaerobically to ethene or ethane may be effective.
Clostridium bifermentans DPH-1;dechlorination;tetrachloroethylene;monoculture;bioreactor;
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Afroza, H. S., Hasegawa, Y., Nomura, I., Chang, Y.C., Sato, T. and Takamizawa, K. : Evaluation of different culture conditions of Clostridium bifermentans DPH-1 for cost effective PCE degradation. Biotechnology and Bioprocess Engineering 10, 40-46, 2005. crossref(new window)

Aulenta, F., Majone, M., Verbo, P. and Tandoi, V. : Complete dechlorination of tetrachloroethene to ethane in presence of methanogenesis and acetogenesis by an anaerobic sediment microcosm. Biodegradation 13, 411-424, 2002. crossref(new window)

Aulenta, F., Fina, A., Potalivo, M., Papini, M. P., Rossetti, S. and Majone, M. : Anaerobic transformation of tetrachloroethane, perchloroethylene, and their mixtures by mixed-cultures enriched from contaminated soils and sediments. Water Science and Technology, 52, 357-362, 2005.

Aulenta, F., Di Tomassi, C., Cupo, C., Papini, M. P. and Majone, M. : Influence of hydrogen on the reductive dechlorination of tetrachloroethene (PCE) to ethene in a methanogenic biofilm reactor: Role of mass transport phenomena. Journal of Chemical Technology & Biotechnology, 81, 1520-1529, 2006. crossref(new window)

Brewster, C. Jr, Cherry, J. A. and Gillham, R. W. : A PCE groundwater plume discharging to a river: influence of the streambed and near-river zone on contaminant distributions. Journal of Contaminant Hydrology, 73, 249-279, 2004. crossref(new window)

Cabirol, N., Jacob, F., Perrier, J., Fouillet, B. and Chambon, P. : Complete degradation of high concentrations of tetrachloroethylene by a methanogenic consortium in a fixed-bed reactor. Journal of Biotechnology 62, 133-141, 1998. crossref(new window)

Carter, S. R. and Jewel, W. J. : Biotransformation of tetrachloroethylene by anaerobic attached-films at low temperatures. Water Research, 27, 607-615, 1993. crossref(new window)

Chang, Y. C., Hatsu, M., Jung, K., Yoo, Y. S. and Takamizawa, K. : Isolation and characterization of a tetrachloroethylene dechlorinating bacterium, Clostridium bifermentans DPH-1. Journal of Bioscience and Bioengineering, 89, 489-491, 2000. crossref(new window)

Cole, J. R., Fathepure, B. Z. and Tiedje, J. M. : Tetrachloroethene and 3-chlorobenzoate dechlorination activities are co-induced in Desulfomonile tiedje DCB-1. Biodegradation 6, 167-172, 1995. crossref(new window)

Christiansen, N., Christensen, S. R., Arvin, E. and Ahring, B. K. : Transformation of tetrachloroethene in an upflow anaerobic sludgeblanket reactor. Applied Microbiology and Biotechnology, 47, 91-94, 1997. crossref(new window)

Cupples, A. M., Spormann, A. M. and McCarty, P. L. : Comparative evaluation of chloroethene dechlorination to ethene by Dehalococcoides-like microorganisms. Environmental Science & Technology, 38, 4768-4774, 2004. crossref(new window)

Damborsky, J : Tetrachloroethene-Dehalogenating Bacteria. Folia Microbiologica, 44, 247-262, 1999. crossref(new window)

DiStefano, T. D., Gossett, J. M. and Zinder, S. H. : Reductive dechlorination of high concentration of tetrachloroethene to ethane by an anaerobic enrichment culture in the absence of methanogenesis. Applied and Environmental Microbiology, 57, 2287-2292, 1991.

deBruin, W. P., Kotterman, M. J. J., Posthumus, M. A., Schraa, G. and Zehnder, A. J. B. : Complete biological reductive transformation of tetrachloroethene to ethane. Applied and Environmental Microbiology, 58, 1996-2000, 1992.

Eisenbeis, M., Bauer-Kreisel, P. and Scholz-Muramatsu, H. : Studies on the dechlorination of tetrachloroethene to cis-1,2-dichloroethene by Dehalospirillum multivorans in biofilms. Water Science and Technology, 36, 191-198, 1997.

Ensley, B. D. : Biochemical diversity of trichloroethylene metabolism. Annual Review of Microbiology, 45, 283-299, 1991. crossref(new window)

Fathepure, B. Z., Nengu, J. P. and Boyd, S. T. : Anaerobic bacteria that dechlorinate perchloroethene. Applied and Environmental Microbiology, 53, 2671-2674, 1987.

Fathepure, B. Z. and Vogel, T. M. : Complete degradation of polychlorinated hydrocarbons by a twostage biofilm reactor. Applied and Environmental Microbiology, 57, 3418-3422, 1991.

Fetzener, S. : Bacterial dehalogenation. Applied Microbiology and Biotechnology, 50, 633-657, 1998. crossref(new window)

Freedman, D. L. and Gossett, G. W. : Biological reductive dechlorination of tetrachloroethylene and trichloroethylene to ethylene under methanogenic conditions. Applied and Environmental Microbiology, 55, 2144-2151, 1989.

Flynn, S. J., Loffler, F. E. and Tiedje, J. M. : Microbial community changes association with a shift from reductive degradation of PCE to reductive degradation of cis-DCE and VC. Environmental Science & Technology, 34, 1056-1061, 2000. crossref(new window)

Gerritse, J., Renard, V., Visser, J. and Gottschal, J. C. : Complete degradation of tetrachloroethene by combining anaerobic dechlorinating and aerobic methanotrophic enrichment cultures. Applied Microbiology and Biotechnology, 43, 920-928, 1995. crossref(new window)

Gerritse, J., Kloetstra, G., Borger, A., Dalstra, G., Alphenaar, A. and Gottschal, J. C. : Complete degradation of tetrachloroethene in coupled anoxic and oxic chemostats. Applied Microbiology and Biotechnology, 48, 553-562, 1997. crossref(new window)

Hirl, P. J. and Irvine, R. L. : Reductive dechlorination of perchloroethylene using anaerobic sequencing batch biofilm reactors (AnSBBR). Water Science and Technology, 35, 49-56, 1997.

Hoelen, T. P., Cunningham, J. A., Hopkins, G. D., Lebron, C. A. and Reinhard, M. : Bioremediation of cis-DCE at a sulfidogenic site by amendment with propionate. Ground Water Monitoring and Remediation, 26, 82-91, 2006.

Holliger, C., Sehraa, G., Stams, A. J. M. and Zehnder, A. J. B. : A high purified enrichment culture couples the reductive dechlorination of tetrachloroethene to growth. Applied and Environmental Microbiology, 59, 2991-2997, 1993.

Hobber, C., Christiansen, N., Arvin, E. and Ahring, B. K. : Improved dechlorinating incorporation of Dehalospirillum multivorans into granular sludge. Applied and Environmental Microbiology, 64, 1860-1863, 1998.

Isalou, M., Sleep, B. E. and Liss, S. N. : Biodegradation of high concentrations of tetrachloroethene in a continuous flow column system. Environmental Science & Technology, 32, 3579-3585, 1998. crossref(new window)

Hata, J., Miyata, N., Kim, E. S., Takamizawa, K. and Iwahori, K. : Anaerobic degradation of cisdichloroethylene and vinyl chloride by Clostridium sp. strain DC1 isolated from landfill leachate sediment. Journal of Bioscience and Bioengineering, 97, 196-201, 2004. crossref(new window)

Komatsu, T., Shinmyo, J. and Momonoi, K : Reductive transformation of tetrachloroethy1ene to ethylene and ethane by an anaerobic filter. Water Science and Technology, 36, 125-132, 1997.

Koziollet, P. D., Bryniok, D. and Knackmuss, H. J. : Ethene as an auxiliary substrate for the cooxidation of cis-1,2-dichloroethene and vinyl chloride. Archives of Microbiology, 172, 240-246, 1999. crossref(new window)

Lee, T. H., Tokunaga, T., Suyama, A. and Furukawa, K. : Efficient dechlorination of tetrachloroethylene in soil slurry by combined use of an anaerobic Desulfitobacterium sp. strain Y-51 and zero-valent iron. Journal of Bioscience and Bioengineering, 92, 453-458, 2001. crossref(new window)

Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randell, R. J. : Protein measurement with the folin phenol reagent. Journal of Biological Chemistry, 193, 265-275, 1951.

Ma, X., Novak, P. J., Clapp, L. W., Semmens, M. J. and Hozalski, R. M. : Evaluation of hollow-fiber polyethylene membranes for hydrogen delivery to support reductive dechlorination in a soil column. Water Research, 37, 2905-2918, 2003. crossref(new window)

Ma, X., Novak, P. J., Semmens, M. J., Clapp, L. W. and Hozalski, R. M. : Comparison of pulsed and continuous addition of $H_2$ gas via membranes for stimulating PCE biodegradation in soil columns. Water Research, 40, 1155-1166, 2006. crossref(new window)

Malachowsky, K. J., Phelps, T. J., Teboli, A. B., Minikin, D. E. and White, D. C. : Aerobic mineralization of trichloroethylene, vinyl chloride, and aromatic compounds by Rhodococcus species. Applied and Environmental Microbiology, 60, 542-548, 1994.

Maymo-Gatell, X., Tandoi, V., Gossett, J. M. and Zinder, S. H. : Characterization of an $H_{2}$-utilizing enrichment culture that reductively dechlorinates tetrachloroethene to vinyl chloride and ethene in the absence of methanogenesis and acetogenesis. Applied and Environmental Microbiology, 61, 3928-3933, 1995.

Maymo-Gatell, X., Chien, Y. T., Gossett, J. M. and Zinder, S. H. : Isolation of a bacterium that reductively dechlorination tetrachloroethene to ethane. Science 276, 1568-1571, 1997. crossref(new window)

Maymo-Gatell, X., Nijenhuis, I. and Zinder, S. H. : Reductive dechlorination of cis-1,2-dichloroethene and vinyl chloride by "Dehalococcoides ethenogenes". Environmental Science & Technology, 35, 516-521, 2001. crossref(new window)

McCarty, P. L. : Breathing with chlorinated solvents. Science 276, 1521-1522, 1997. crossref(new window)

Mendoza-Sanchez, I., Autenrieth, R. L., McDonald, T. J. and Cunningham, J. A. : Effect of pore velocity on biodegradation of cis-dichloroethene (DCE) in column experiments. Biodegradation 21, 365-377, 2010. crossref(new window)

Muenzner, H. D., Clapp, L. W., Hozalski, R. M., Semmens, M. J. and Novak, P. J. : Dechlorination of PCE by mixed methanogenic cultures using hollow- fiber membranes. Bioremediation Journal, 6, 337-350, 2002. crossref(new window)

Noftsker, C. and Watwood, M. E. : Removal of tetrachloroethylene in an anaerobic column bioreactor. Applied Microbiology and Biotechnology, 48, 424-430, 1997. crossref(new window)

Parsons, F., Wood, P. R. and Demarco, J. J. : Transformation of tetrachloroethane in microcosms and groundwater. Journal of the American Water Works Association, 76, 56-59, 1984.

Parakash, S. M. and Gupta, S. K. : Biodegradation of tetrachloroethylene in upflow anaerobic sludge blanket reactor. Bioresource Technology, 72, 47-54, 2000. crossref(new window)

Rosenthal, H., Adrian, L. and Steiof, M. : Dechlorination of PCE in the presence of $Fe^{0}$ enhanced by a mixed culture containing two Dehalococcoides strains. Chemosphere 55, 661-669, 2004. crossref(new window)

Ryoo, D., Shim, H., Canada, K., Barbieri, P. and Wood, T. K. : Aerobic degradation of tetrachloroethylene by toluene-o-xylene monooxygenase of Pseudomonas stuzeri OX1. Nature Biotechnology 18, 775-778, 2000. crossref(new window)

Schmidt, J. E. and Ahring, B. K. : Granular sludge formation in upflow anaerobic sludge blanket (UASB) reactors. Biotechnology and Bioengineering, 49, 229-249, 1996.

Scholz-Muramatsu, H., Szewzyk, R., Szewzyk, U. and Gaiser, S. : Tetrachloroethylene as electron acceptor for the anaerobic degradation of benzoate. FEMS Microbiology Letters, 66, 81-86, 1990. crossref(new window)

Scholz-Muramatsu, H., Neumann, A., Messmer, M., Moore, E. and Diekert, G. : Isolation and characterization of Dehalospirillum multivorans gen. nov., sp. nov., a tetrachloroethene-utilizing, strictly anaerobic bacterium. Archives of Microbiology, 163, 48-56, 1995. crossref(new window)

Sharma, O. K. and McCarty, P. L. : Isolation and characterization of a facultative aerobic bacterium that reductively dehalogenates tetrachloroethene to cis-1,2-dichloroethene. Applied and Environmental Microbiology, 62, 761-765, 1996.

Suyama, A., Iwakiri, R., Kai, K., Tokunaga, T., Sera, N. and Furukawa, K. : Isolation and characterization of Desulfitobacterium sp. strain Y51 capable of efficient dechlorination of tetrachloroethene and polychloroethanes. Bioscience, Biotechnology, and Biochemistry, 65, 1474-1481, 2001. crossref(new window)

U.S. EPA : Superfund NPL Characterization Project: National Results. U.S. Environmental Protection Agency, Office of Emergency and Remedial Response. EPA/540/8-91/069, 1991.

U.S. EPA : National revised primary drinking water regulations, volatile synthetic organic chemicals in drinking water: advanced notice of proposed rulemaking. Federal Register, 47, 9349-9358, 1982.

Vogel, T. M. and McCarty, P. L. : Biotransforination of tetrachloroethylene to trichloroethylene, dichloroethylene, vinyl chloride, and carbon dioxide under methanogenic conditions. Applied and Environmental Microbiology, 49, 1080-1083, 1985.

Wild, A. P., Winkelbauer, W. and Leisinger, T. : Anaerobic dech1orination of trichloroethene, tetrachloroethene and 1,2-dichloroethane by an acetogenic mixed culture in a fixed-bed reactor. Biodegradation 6, 309-318, 1995. crossref(new window)

Wu, W. M., Nye, J., Hickey, R. F. and Zeikus, J. G. : Dechlorination of PCE and TCE to ethene using an anaerobic microbial consortium. In: Bioremediation of chlorinated solvents. Hinchee, R.E., Leeson, A. and Semprini, L. (eds), Battelle, Columbus Richland, pp.45-52, 1995.

Yamamoto, K., Fukushima, M., Kakutani, N. and Tsuruho, K. : Contamination of vinyl chloride in shallow urban river in Osaka, Japan. Water Research, 35, 561-566, 2001. crossref(new window)