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DEHP, DEP and DBP Exposure Analysis using Urinary Metabolites of Gyonggi Province University Students
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 Title & Authors
DEHP, DEP and DBP Exposure Analysis using Urinary Metabolites of Gyonggi Province University Students
Lee, JangWoo; Kho, YoungLim; Kim, SungKyoon; Choi, Kyungho; Hwang, SeongHee; Jeong, Jeeyeon; Kim, Pangyi;
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Objectives: Phthalates are used as plasticizers in polyvinyl chloride (PVC) plastics. As phthalate plasticizers are not chemically bound to the PVC, they can leach, migrate or evaporate into indoor air and atmosphere, foodstuffs, other materials, etc. Therefore, humans are exposed through ingestion, inhalation, and dermal exposure over their entire lifetime, including during intrauterine development. In particular, university students have a great number of opportunities to contact products including phthalates during campus life (food packaging, body care products, cosmetic, lotions, aftershave, perfume etc.). The purpose of this study was to examine levels of phthalate exposure as undergraduate students begin to use pharmaceuticals and personal care products including phthalates. Methods: Phthalate metabolites, mono-ethyl phthalate (MEP), mono-n-butyl phthalate (MnBP), mono-isobutyl phthalate (MiBP), mono-2- ethylhexyl phthalate (MEHP), {(mono-(2-ethyl-5-hydroxyhexyl) phthalate (MEHHP}, and mono-(2-ethlyl-5-oxohexyl) phthalate (MEOHP} were examined. 80 urine samples collected from university students were analyzed using LC/MS/MS(API 4000, Applied Bioscience) with on-line enrichment and columnswitching techniques. This study was carried out at Y university located in Gyonggi Province from 2008 to 2011. Results: The detection limit of phthalate metabolites were 0.03 ng/mL for MEP, 0.11 ng/mL for MnBP, 0.08 ng/mL for MiBP, 0.93 ng/mL for MEHP, 0.19 ng/mL for MEOHP and 0.16ng/mL for MEHHP. MnBP showed the highest urinary levels (median: 31.6 ug/L, 24.8 ug/g creatinine (cr)). Concentrations were also high for MEHHP (median: 24.1 ug/L, 19.0 ug/g cr), followed by MEOHP (median: 22.8 ug/L, 17.9 ug/g cr). In individual cases, the maximum level reached up to 348 ug/L, and 291 ug/g cr, respectively. The urinary and creatinine adjusted levels of MEP were lower than those for DBP and DEHP metabolites, but were higher in 95th percentiles. As a result, the mean daily DEP intake value was 2.3 bw/day, 3.5 bw/day for DEHP and 4.9 bw/day for DBP. Conclusion: These students' phthalate exposure levels were below the international safe level set by the EU, but higher than the 2012 KFDA survey of the age group from 3 to 18.
Phthalates;university student;daily intake;urine;metabolites;
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Hiroaki Itoh, Kikuo Yoshida, Shigeki Masunaga. Evaluation of the effect of governmental control of human exposure to two phthalates in Japan using a urinary biomarker approach. International Journal of Hygiene Environmental Health. 2005; 208: 237-245. crossref(new window)

CDC, (2005). Third National Report on Human Exposure to Environmental Chemicals. Department of Health and Human Services. Centers for Disease Control and Prevention (CDC), Atlanta, GA.

Ted Schettler. Human exposure to phthlalates via consumer products. International Journal of Andrology, 2006; 29: 134-139. crossref(new window)

Ministry of Environment Republic of Korea (2006), The amount of endocrine disruptor in 2006.

Hauser, R., Calafat, A. Phthalates and human health. Occupational Environmental Medicine 2005; 62: 806-818. crossref(new window)

Poon, R., Lecavalier, P., Mueller, R., Valli, V.E., Procter, B.G., Chu, I. Subchronic oral toxicity of di-n-octyl phthalate and di(2-ethylhexyl) phthalate in the rat. Food and Chemical Toxicology 1997; 35: 225-239. crossref(new window)

Lovekamp-Swan, T., Davis, B. Mechanisms of phthalate ester toxicity in the female reproductive system. Environmental Health Perspectives 2003; 111, 139-145.

Akingbemi, B.T., Ge, R., Klinefelter, G.R., Zirkin, B.R., Hardy, M.P. Phthalate-induced leydig cell hyperplasia is associated with multiple endocrine disturbances. Proceeding National Academy of Science, 2004; 101: 775-780. crossref(new window)

CERHR. (2000). National Toxicology Program- .CERHR Expert Panel Report on Di-n-Butyl Phthalate. NTP Center for the Evaluation of Risks to Human Reproduction, NC.

Duty, S.M., Silva, M.J., Barr, D.B., Brock, J.W., Ryan, L., Chen, Z., Herrick, R.F., Christiani, D.C., Hauser, R. Phthalate exposure and human semen parameters. Epidemiology 2003; 14: 269-277.

Swan, S.H., Main, K.M., Liu, F., Stewart, S.L., Kruse, R.L., Calafat, A., Mao, C.S., Redmon, J.B., Ternand, C.L., Sullivan, S., Teague, J.L., and the Study for Future Families Research Team. Decrease in anogenital distance among male infants with prenatal phthalate exposure. Environmental Health Perspectives 2005; 113: 1056-1061. crossref(new window)

Blount, B.C., Silva, M.J., Caudill, S.P., Needham, L.L., Pirkle, J.L., Sampson, E.J., Lucier, G.W., Jackson, R.J., Brock, J.W. Levels of seven urinary phthalate metabolites in a human reference population. Environmental Health Perspectives 2000a,b; 108, 979-982.

Koch, H.M., Rossabach, B., Drexler, H., Angerer, J. Internal exposure of the general population to DEHP and other phthalates-determination of secondary and primary phthalate monoester metabolites in urine. Environmental Research 2003c; 93: 177-185. crossref(new window)

Sneed J, Holdt CS. Many factors influence college students' eating patterns. Journal of American Diet Association, 1991; 91: 1380.

Hertzler AA, Frary RB. Family factors and fat consumption of college students. Journal of American Diet Association, 1996; 96: 711-4. crossref(new window)

David, R.M. Exposure to phthalate esters. Environmental Health Perspectives 2000; 108: A440.

Koch, H.M., Drexler, H., Angerer, J. An estimation of the daily intake of di(2-ethylhexyl)phthalate (DEHP) and other phthalates in the general population. International Journal of Hygiene and Environmental. Health 2003a; 206: 77-83. crossref(new window)

Kohn MC, Parham F, Masten SA, Portier CJ, Shelby MD, Brock JW, Needham LL. Human exposure estimates for phthalates. Environmental Health Perspectives 2000; 108: A440-A442.

Calafat AM, McKee RH, Integrating biomonitoring exposure data into the risk assessment process : phthalate [diethyl phthalate and di(2-ethylhexyl) phthalate] as a case study. Environmental Health Perspectives 2006; 114: 1783-1789.

Kessler, W., Phokha, W., Csanady, G.A., Filser, J.G. No background concentrations of di(2-ethylhexyl) phthalate and mono(2-ethylhexyl)phthalate in blood of rats. Archives of Toxicology. 2001; 75(1): 62-64. crossref(new window)

Anderson, W.A., Castle, L., Scotter, M.J., Massey, R.C., Springall, C. A biomarker approach to measuringhuman dietary exposure to certain phthalate diesters. Food Additives and Contaminants. 2001; 18(12): 1068-1074. crossref(new window)

Silva, M.J., Slakman, A.R., Reidy, J.A., Preau Jr., J.L., Herbert, A.R., Samandar, E., Needham, L.L., Calafat, A.M. Analysis of human urine for fifteen phthalate metabolites using automated solid-phase extraction. Journal of Chromatography. B Analytical Technology in Biomedical Life Science. 2004b; 805(1): 161-167. crossref(new window)

Suzuki, T., Yaguchi, K., Suzuki, S., Suga, T. Monitoring of phthalic acid monoesters in river water by solid-phase extraction and GC-MS determination. Environmental Science and Technololgy. 2001; 35(18): 3757-3763. crossref(new window)

Barr, D.B., Silva, M.J., Kato, K., Reidy, J.A., Malek, N.A., Hurtz, D., Sadowski, M., Needham, L.L., Calafat, A.M. Assessing human exposure to phthalates using monoesters and their oxidized metabolites as biomarkers. Environmental Health Perspectives 2003; 111(9): 1148-1151. crossref(new window)

Dirven, H.A., van den Broek, P.H., Jongeneelen, F.J., Determination of four metabolites of the plasticizer di(2-ethlyhexyl) phthalate in human urine samples. International Archives in Occupational and Environmental Health. 1993; 64(8): 555-560. crossref(new window)

Preuss, R., Koch, H.M., Angerer, J. Biological monitoring of the five major metabolites of di(2- ethlyhexyl) phthalate (DEHP) in human urine using column- switching liquid chromatography-tandem mass spectrometry. Journal Chromatograpy. 2005; B816: 269-280.

Kato, K., Silva, M.J., Reidy, J.A., Hurtz III, D., Malek, N.A., Needham, L.L., Nakazawa, H., Barr, D.B., Calafat, A.M. Mono(2-ethyl-5-hydroxyhexyl) phthalate and mono-(2-ethyl-5-oxohexyl) phthalate as biomarkers for human exposure assessment to di-(2-ethylhexyl) phthalate. Environmental Health Perspectives. 2004; 112(3): 327-330.

Schettler T. Human exposure to phthalates via consumer products. International Journal Andrology, 2006; 29(1): 134-9, discussion 81-5. crossref(new window)

Wormuth M, Scheringer M, Vollenweider M, Hungerbuhler K. What are the sources of exposure to eight frequently used phthalic acid esters in Europeans? Risk Analysis, 2006; 26(3): 803-24. crossref(new window)

Elsisi AE, Carter DE, Sipes IG. Dermal absorption of phthalate diesters in rats. Fundamental and Applied Toxicology, 1989; 12(1): 70-7. crossref(new window)

Janjua NR, Mortensen GK, Andersson AM, Kongshoj B, Skakkebaek NE, Wulf HC. Systemic uptake of diethyl phthalate, dibutyl phthalate, and butyl paraben following whole-body topical application and reproductive and thyroid hormone levels in humans. Environmental Science and Technology, 2007; 41(15): 5564-70. crossref(new window)

Kho, Y.L, Jeong, J.Y., Choi, K.H, Kim, P.G., Determination of phthalate metabolites in Korean children's urine by HPLC with triple quadrupole tandem mass spectrometry. Journal of Environmental Health Sciences. 34(4), 271-278, 2008. crossref(new window)

CDC, (2009). Forth National Report on Human Exposure to Environmental Chemicals. Department of Health and Human Services. Centers for Disease Control and Prevention (CDC), Atlanta, GA.

Matthias Wittassek, Gerhard Andress Wiesmuller, Holger Martin Koch, Rolf Eckard, Lorenz Dobler, Johannes Muller, Jurgen Angerer, Christoph Schluter. Internal phthalate exposure over the last two decades - A retrospective human biomonitoring study. International Journal of Hygiene and Environmental Health 2007; 210: 319-333. crossref(new window)

Koch, H.M., Becker, K., Wittassek, M., Seiwert, M., Angerer, J., Kolossa-Gehring, M., 2007a. Din- butylphthalate (DnBP) and butylbenzylphthalate (BBzP).urinary metabolite levels and estimated daily intakes: pilot study for the German Environmental Survey on children (GerES IV). Journal of Exposure Science and Environmental Epidemiology, published online 27 September 2006, doi:10.1038/sj.jes.7500526. crossref(new window)