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Phylogenetic Groups and Virulence Factors of Escherichia coli Causing Urinary Tract Infection in Children
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  • Journal title : Pediatric Infection and Vaccine
  • Volume 22, Issue 3,  2015, pp.194-200
  • Publisher : The Korean Society of Pediatric Infectious Diseases
  • DOI : 10.14776/piv.2015.22.3.194
 Title & Authors
Phylogenetic Groups and Virulence Factors of Escherichia coli Causing Urinary Tract Infection in Children
Kim, Ji Mok; Cho, Eun Young; Lee, Jae Ho;
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 Abstract
Purpose: Urinary tract infection (UTI) is a common bacterial infection in children and Escherichia coli is a predominant pathogen. The purpose of this study is to evaluate phylogenetic groups and virulence factors of E. coli causing UTI in children in Korea. Methods: From October 2010 to April 2013, urinary E. coli strains were isolated from the 33 pediatric patients of UTI. Multiplex polymerase chain reactions were performed to evaluate the phylogenetic groups and 5 virulence factor genes (fimH, sfa, papA, hylA, and cnf1) of E. coli. Distribution of molecular characteristics of E. coli was analyzed by clinical diagnosis and accompanying vesicoureteral reflux (VUR). Results: Most (84.8%) uropathogenic E. coli were belonged to phylogenetics group B2 and the others (15.2%) were belonged to group D. The virulence factors were distributed as: fimH (100%), sfa (100%), hylA (63.6%), cnfI (63.6%), and papA (36.4%). According to clinical diagnosis, phylogenetic distribution of E. coli strain was 92.3% of B2 and 7.7% of D in acute pyelonephritis and 57.1% of B2 and 42.9% of D in cystitis. Distribution of virulence factors was similar in both groups. In patients with acute pyelonephritis, phylogenetic distribution was similar in VUR and non-VUR group, but proportion of papA genes were lower in VUR group than that of non-VUR group (43.8% vs. 20.0%, P=0.399). Conclusions: This study provides current epidemiologic molecular data of E. coli causing pediatric UTI in Korea and will be a fundamental for understanding the pathogenesis of pediatric UTI.
 Keywords
Escherichia coli;Virulence factor;Phylogenetic groups;Acute pyelonephritis;
 Language
Korean
 Cited by
1.
ST131 in urinary tract infections in children, Korean Journal of Pediatrics, 2017, 60, 7, 221  crossref(new windwow)
 References
1.
Shaikh N, Morone NE, Bost JE, Farrell MH. Prevalence of urinary tract infection in childhood: a meta-analysis. Pediatr Infect Dis J 2008;27:302-8. crossref(new window)

2.
Roberts KB, Akintemi OB. The epidemiology and clinical presentation of urinary tract infections in children younger than 2 years of age. Pediatr Ann 1999;28:644-9. crossref(new window)

3.
Faust WC, Diaz M, Pohl HG. Incidence of post-pyelonephritic renal scarring: a meta-analysis of the dimercapto-succinic acid literature. J Urol 2009;181:290-8.

4.
Park YS. Renal scar formation after urinary tract infection in children. Korean J Pediatr 2012;55:367-70. crossref(new window)

5.
Lee YJ, Lee JH, Park YS. Risk factors for renal scar formation in infants with first episode of acute pyelonephritis: a prospective clinical study. J Urol 2012;187:1032-6. crossref(new window)

6.
Pecile P, Miorin E, Romanello C, Vidal E, Contardo M, Valent F, et al. Age-related renal parenchymal lesions in children with first febrile urinary tract infections. Pediatrics 2009;124:23-9. crossref(new window)

7.
Jung HJ, Aum JA, Jung SJ, Huh JW. Different characteristic between Escherichea coli and non-Escherichea coli urinary tract infection. Korean J Pediatr 2007;50:457-61. crossref(new window)

8.
Foxman B. Epidemiology of urinary tract infections: incidence, morbidity, and economic costs. Am J Med 2002;113: 5-13.

9.
Bien J, Sokolova O, Bozko P. Role of uropathogenic Escherichia coli virulence factors in development of urinary tract infection and kidney damage. Int J Nephrol 2012;2012:681473.

10.
Bonacorsi S, Houdouin V, Mariani-Kurkdjian P, Mahjoub-Messai F, Bingen E. Comparative prevalence of virulence factors in Escherichia coli causing urinary tract infection in male infants with and without bacteremia. J Clin Microbiol 2006;44:1156-8. crossref(new window)

11.
Lee DG, Cho JJ, Park HK, Kim DK, Kim JI, Chang SG, et al. Preventive effects of hyaluronic acid on Escherichia coli -induced urinary tract infection in rat. Urology 2010;75:949-54. crossref(new window)

12.
Chiou YY, Chen MJ, Chiu NT, Lin CY, Tseng CC. Bacterial virulence factors are associated with occurrence of acute pyelonephritis but not renal scarring. J Urol 2010;184:2098-102. crossref(new window)

13.
Billips BK, Forrestal SG, Rycyk MT, Johnson JR, Klumpp DJ, Schaeffer AJ. Modulation of host innate immune response in the bladder by uropathogenic Escherichia coli . Infect Immun 2007;75:5353-60. crossref(new window)

14.
Schilling JD, Mulvey MA, Hultgren SJ. Dynamic interactions between host and pathogen during acute urinary tract infections. Urology 2001;57:56-61.

15.
Song J, Abraham S. Innate and adaptive immune responses in the urinary tract. Eur J Clin Invest 2008;38:21-8. crossref(new window)

16.
Clermont O, Bonacorsi S, Bingen E. Rapid and simple determination of the Escherichia coli phylogenetic group. Appl Environ Microbiol 2000;66:4555-8. crossref(new window)

17.
Tarchouna M, Ferjani A, Ben-Selma W, Boukadida J. Distribution of uropathogenic virulence genes in Escherichia coli isolated from patients with urinary tract infection. Int J Infect Dis 2013;17:e450-3. crossref(new window)

18.
Newman TB, Bernzweig JA, Takayama JI, Finch SA, Wasserman RC, Pantell RH. Urine testing and urinary tract infections in febrile infants seen in office settings: the Pediatric Research in Office Settings' Febrile Infant Study. Arch Pediatr Adolesc Med 2002;156:44-54. crossref(new window)

19.
Mak RH, Kuo HJ. Pathogenesis of urinary tract infection: an update. Curr Opin Pediatr 2006;18:148-52. crossref(new window)

20.
Johnson JR, Owens K, Gajewski A, Kuskowski MA. Bacterial characteristics in relation to clinical source of Escherichia coli isolates from women with acute cystitis or pyelonephritis and uninfected women. J Clin Microbiol 2005;43:6064-72. crossref(new window)

21.
Tiba MR, Yano T, Leite Dda S. Genotypic characterization of virulence factors in Escherichia coli strains from patients with cystitis. Rev Inst Med Trop Sao Paulo 2008;50:255-60. crossref(new window)

22.
Houdouin V, Bonacorsi S, Mahjoub-Messai F, Mariani- Kurkdjian P, Bidet P, Sebag G, et al. Phylogenetic groups and virulence factors of Escherichia coli strains causing pyelonephritis in children with and without urinary tract abnormalities. Clin Microbiol Infect 2007;13:740-2. crossref(new window)

23.
Brauner A, Jacobson SH, Kuhn I. Urinary Escherichia coli causing recurrent infections--a prospective follow-up of biochemical phenotypes. Clin Nephrol 1992;38:318-23.

24.
Foxman B, Gillespie B, Koopman J, Zhang L, Palin K, Tallman P, et al. Risk factors for second urinary tract infection among college women. Am J Epidemiol 2000;151:1194-205. crossref(new window)

25.
Ikahelmo R, Siitonen A, Heiskanen T, Karkkainen U, Kuosmanen P, Lipponen P, et al. Recurrence of urinary tract infection in a primary care setting: analysis of a 1-year follow-up of 179 women. Clin Infect Dis 1996;22:91-9. crossref(new window)