JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Diagnosis of Bovine Theileriosis by Direct PCR and Electrophoresis from Whole Blood Without DNA Extraction
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Diagnosis of Bovine Theileriosis by Direct PCR and Electrophoresis from Whole Blood Without DNA Extraction
Seong Ho Kang; Sangmin Jang; Joon-Seok Chae; Yongseong Kim;
  PDF(new window)
 Abstract
We have developed a direct polymerase chain reaction (PCR) and electrophoresis method for the diagnosis of bovine theileriosis from whole blood DNA analysis without DNA extraction. The technique empolyed a FoLT (formamide, low temp.) technique and was utilized in the diagnosis of bovine theileriosis. Formamide solubilize the blood cells, and the lowered incubation temperatures reduced protein coagulation. 100-200 nL of whole blood and PCR reagents were introduced directly into a PCR tube. After the amplification, the PCR product (816-bp DNA) was introduced into the electrophoresis system. The results of this analysis were consist with those obtained using purified DNA.
 Keywords
T. buffeli(buffeli/orientalis/sergenti);Direct PCR;Whole Blood;Diagnosis;
 Language
Korean
 Cited by
1.
Fast Diagnosis of Bovine Theileriosis by Whole Blood PCR and Microchip Electrophoresis,;;;;

Bulletin of the Korean Chemical Society, 2004. vol.25. 5, pp.757-760 crossref(new window)
1.
On-channel base stacking in microchip capillary gel electrophoresis for high-sensitivity DNA fragment analysis, Journal of Chromatography A, 2005, 1064, 1, 121  crossref(new windwow)
 References
1.
Gubbels, M.J.; Hong. Y.; van der Weide, M.; Qi, B.; Nijman, I.J.; Guangyuan, L.; Jongejan, F. Int. J. Parasitol.2000, 30, 943-952. crossref(new window)

2.
Gubbels, M.J.; Yin, H.; Bai, Q.; Liu, G.; Nijman, I. J.; Jongejan, F. Parasitol Res, 2002, 88(13 Suppl 1), S28-32. crossref(new window)

3.
Onuma, M.; Kakuda, T.; Sugimoto, C. Comp. Immun. Microbiol. Infect. Dis., 1998, 21(3), 165-177. crossref(new window)

4.
채준석; 이주묵, 권오덕, 이승옥, 채건상. 대한수의학회지, 1996, 36, 187-193. crossref(new window)

5.
이주묵, 김명철. 대한수의학회지, 1989, 27(2), 321-330.

6.
Nam, C. R.; Cheol, D. Y. Diagnosis of veteriary hemoparasitic disease; Tokyo modern print; 1986, p 149-169. crossref(new window)

7.
김 두. 대한수의학회지, 1993, 33(1), 137-143.

8.
Choi, E. J.; Kang, S. W.; Kweon, C. H.; Jeong, W. S.; Yoon, Y. D.; Song, H. J. Korean J. Parasitol, 1997, 35(2) 111-117. crossref(new window)

9.
Innis, M. A. PCR protocols: a guide to methods and applications; San Diego, Academic Press; 1990. crossref(new window)

10.
채준석, 이주묵, 권오덕, 이승옥, 채건상. 대한수의학회지, 1996, 36(1), 187-193. crossref(new window)

11.
Gross-Bellard, N.; Oudet, P.; Chambon, P. Eur. J. Biochem.1973, 36, 32-38. crossref(new window)

12.
Miller, S. A.; Dykes, D. D.; Polesky, H. F. Nucl. Acids Res. 1998, 16, 1215. crossref(new window)

13.
Klintschar, M; Neuhuber, F. J. Forensic Sci. 2000,45(3) 669-673. crossref(new window)

14.
Ferre, F.; Garduno, F. Nucl. Acids. Res. 1989, 17, 2141. crossref(new window)

15.
Casareale, D.; Pottathil, R.; Diaco, R. PCR Methods and Applications, Cold Spring Harbor Laboratory Press; 1992; p 149-153.

16.
Mercier, B.; Gaucher, C.; Feugeas, O.; Mazurier, C.Nucl. Acids Res. 1990, 18(19), 5908. crossref(new window)

17.
McCusker, J.; Dawson, M. T.; Noone, D.; Gannon, F.; Smith, T. Nucl. Acids Res. 1992, 20(24), 6747. crossref(new window)

18.
Panaccio, M.; Georgesz, M.; Hollywell, C.; Lew, A.Nucl. Acids Res. 1993, 21(19), 4656. crossref(new window)

19.
Makowski, G. S.; Davis, E. L.; Aslanzadeh, J.; Hopfer, S. M. Nucl. Acids Res. 1995, 23(18), 3788-3789. crossref(new window)

20.
Grevelding, C. G.; Kampkotter, A.; Hollmann, M.; Schafer, U.; Kunz, W. Nucl. Acids Res. 1996, 24(20),4100-4101. crossref(new window)

21.
Panaccio, M.; French, M.; Lew, A. Methods in Neurosciences,1995, 26 3-14. crossref(new window)

22.
Panaccio, M.; Georgesz, M.; Lew, A. BioTechniques,1993, 14, 238.

23.
Zhang, Y.; He, Y.; Yeung, E. S. Electrophoresis, 2001,22, 2296-2302. crossref(new window)

24.
Michael Panaccio, M.; Lew, A. M. In PCR Technology Current Innovations Griffin, H. G.; Griffin, A. M.; Ed.; CRC Press: USA, 1994; p 151-157.

25.
이양한, 임시근, 강필원, 최동호, 윤송노, 한면수. 한국분석과학회지, 1999, 12(3), 260-264

26.
QIAGEN, QIAamp DNA Mini Kit and QIAamp DNA Blood Mini Kit Handbook, QIAGEN: USA, September2001, p 27-29. crossref(new window)

27.
Panaccio, M.; Lew, A. Nucleic Acids Res. 1991, 19, 1151. crossref(new window)