JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Activity and Stability of Alcohol Oxidase from Hansenula sp. by Electrostimulation
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Activity and Stability of Alcohol Oxidase from Hansenula sp. by Electrostimulation
Lee, Kang-Min; Kim, Kyung-Suk; Park, Chung-Ung;
  PDF(new window)
 Abstract
We investigated the activity and stability of alcohol oxidase from Hansenula sp. under the electric stimulation. The activity and stability of alcohol oxidase depended on electric output voltage, stimulation time, pulse duration and pulse interval. This inactivation of the enzyme under electric stimulation could be recovered by stabilizing additives such as sugars, polymers and hydrogels. The enzyme activity retained about 52% in 10% trehalose solution under electric stimulation with 40 V and 10 min. The stabilizing of enzymes against electric stimulation showed a great potential use of enzymes in biotechnology and medical engineering fields.
 Keywords
Electrostimulation;Alcohol Oxidase;Stability;
 Language
Korean
 Cited by
1.
알콜 탈수소효소의 안정도에 미치는 전기 자극의 효과,이강민;김철생;이혜정;함대현;박충웅;

동의생리병리학회지, 2004. vol.18. 6, pp.1723-1727
 References
1.
Hood, D. A.; Zak, R.; Pette, D. Eur. J. Biochem. 1989,179, 275. crossref(new window)

2.
Stebbins, C. L.; Brown, B.; Levin. D.; Longhurst, J.C.J. Appl. Physiology, 1988, 65, 1539. crossref(new window)

3.
Rowley, B. Proc. Soc. Exp. Biolo. Med., 1972. 139. 929. crossref(new window)

4.
Beg, H. Bioelectrochem. Bioenerg. 1995, 38, 153. crossref(new window)

5.
Gamaley, I.; Augsten, K.; Berg, H. Bioelectrochem.Bioenerg. 1995, 38, 415. crossref(new window)

6.
Boteva, R.; Visser, A.; Filippi, B.; Vriend, G.; Veenhuis,M.; Klei, I.J. Biochemistry 1999, 38, 5034. crossref(new window)

7.
Salomons, F.A.; Kiel, J.; Faber, K.; Veenhuis, M.; Klei,I.J. J. Biol. Chem. 2000, 275, 12603. crossref(new window)

8.
Demers, N. Agostinelli, E. Averill-Bates, D.A.; Fortier,G. Biotech. and Appl. Biochem. 2001, 33, 201. crossref(new window)

9.
Basri, M.; Samsudin, S.; Bin, Ahmad M.; Razak, C.;Salleh, A.B. Applied Biochem. and Biotech. 1999, 81,205. crossref(new window)

10.
Akiyoshi, K.; Sasaki, Y.; Sunamoto, J. BioconjugateChemistry 1999, 10, 321. crossref(new window)

11.
Zhang, L.; Li, L.; An, L.; Hoffman, R.M.; Hofmann, G.A.Bioelectrochem. Bioenerg. 1997, 42, 283. crossref(new window)

12.
Gomez, L.; Ramirez, H.; Villalonga, R. BiotechnologyLetters 2000, 22, 347. crossref(new window)

13.
Davidson, P.; Sun, W.Q. Pharmaceutical Research,2001, 18, 474. crossref(new window)

14.
George, S.P.; Ahmad, A.; Rao, M.B. Bioresource Technology,2001, 78, 221 crossref(new window)

15.
Draber, P.; Draberova, E.; Novakova, M. Journal ofImmunological Methods, 1995, 181, 1. crossref(new window)

16.
DePaz, R.A.; Dale, D.A.; Barnett, C.; Carpenter, J.F.;Gaertner, A.; Randolph, T. Enzyme and MicrobialTechnology, 2002, 31, 765. crossref(new window)

17.
Chang, B.S.; Mahoney, R.R. Biotechnology and AppliedBiochemistry, 1995, 22, 203.

18.
Jene, Q.; Pearson, J.C.; Lowe, C.R. Enzyme and MicrobialTechnology 1997, 20, 69. crossref(new window)

19.
Beg, H. Bioelectrochem. Bioenerg. 1995, 38, 153. crossref(new window)

20.
Lei, C.; Berg, H. Bioelectrochem. Bioenerg. 1998, 45, 261. crossref(new window)

21.
Gamaley, I.; Augsten, K.; Berg, H. Bioelectrochem.Bioenerg. 1995, 38, 415. crossref(new window)

22.
Kwee, S.; Rastma, P. Bioelectrochem. Bioenerg. 1995,36, 109. crossref(new window)

23.
Cossarizza, A.; Monti, D.; Bersani, F.; Cadossi, R.; Sacchi,A.; Franceschi C. Biochem. Biophy. Res. Comm. 1989,160, 692. crossref(new window)