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Diagnosis of the ORF Virus Using a Mixture of Sieving Gel Matrixes in Microchip Gel Electrophoresis
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 Title & Authors
Diagnosis of the ORF Virus Using a Mixture of Sieving Gel Matrixes in Microchip Gel Electrophoresis
Kim, Yun-Jeong; Chae, Joon-Seok; Kang, Seong-Ho;
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 Abstract
We have developed a novel polymerase chain reaction (PCR)-microchip gel electrophoresis (MGE) method based on the sieving gel mixture of commercially available poly(vinylpyrrolidone) (PVP) and hydroxy ethyl cellulose (HEC) for the rapid detection and diagnosis of the orf virus (ORFV) from Korean indigenous goat. After amplification of 594-bp DNA fragment from the B2L gene of ORF virus, the amplicon was analyzed by the MGE separation. The glass microfluidic chip (64 mm total length (36 mm effective length)90 m width20 m depth) allowed the fast detection and diagnosis of ORFV in the mixture of 1.0% PVP ( 360,000) and 1.0% HEC (250,000) as a sieving matrix with better resolution and reproducibility of DNA fragments. Under the electric field of 277.8 V/cm, the 594-bp DNA was analyzed within 4 min. Compared to traditional slab gel electrophoresis, the PCR-MGE method was twenty times faster and an effective clinical method for the quantitative analysis of ORFV.
 Keywords
Orf virus;DNA Sieving Matrix;Diagnosis;Microchip Gel Electrophoresis;
 Language
Korean
 Cited by
1.
유전자변형 대두의 빠른 검출을 위한 복합-중합효소연쇄반응/마이크로칩 전기영동법,박미라;이해성;강성호;

대한화학회지, 2005. vol.49. 3, pp.255-260 crossref(new window)
1.
Microchip electrophoretic separation for the fast diagnosis ofAnaplasma phagocytophiluminfection in cattle, Journal of Separation Science, 2010, NA  crossref(new windwow)
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