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The Development of Analysis System for Genes Related Disease Using Chemical Properties of DHPLC
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 Title & Authors
The Development of Analysis System for Genes Related Disease Using Chemical Properties of DHPLC
Kim, Jong-Gyu; Nam, Yun-Hyeong; Park, Sang-Beom; Lee, Jae-Sik; Gang, Won;
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 Abstract
In this study we extracted DNA from 100 tissues of breast cancer patients and 103 normals. Then we confirmed single-nucleotide polymorphism(SNP) using PCR-DHPLC(polymerase chain reaction-denaturing high performance liquid chromatogrphy).Also, we studied SNP of samples using several columns to identify relation between packing materials of column and resolution.As a result, we identified 4 C/A, C/G genotypes(4%) in exon 5 and 37 T del genotypes(37%) in exon 8 among 100 breast cancer tissues and 2 in exon 5, 9 in exon 8 among 103 normal samples.In resolution test, we confirmed that PS-DVB(poly styrene-divinylbenzen) column is more efficient than C18 column.
 Keywords
p53 Gene, Breast Cancer, PCR-DHPLC, Mutation
 Language
Korean
 Cited by
1.
Denaturing HPLC를 이용한 리팜핀 내성 결핵균의 검출,남윤형;이상현;안영창;조민호;장원철;박수민;권필승;김종완;

Annals of Laboratory Medicine, 2008. vol.28. 2, pp.95-102
1.
Detection of Rifampin Resistant Mycobacterium tuberculosis complex using Denaturing HPLC, The Korean Journal of Laboratory Medicine, 2008, 28, 2, 95  crossref(new windwow)
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