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Preparation of Affinity Column Based on ZR4+ Ion forPhosphoproteins Isolation
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 Title & Authors
Preparation of Affinity Column Based on ZR4+ Ion forPhosphoproteins Isolation
Lee, Seon-Mi; Bae, In-Ae; Park, Jung-Hyen; Kim, Tae-Dong; Choi, Seong-Ho;
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This paper has described about preparation of affinity column based on the poly(styrene-co- gly-cidyl methacrylate) prepared by emulsion polymerization of styrene and glycidyl methacrylate in order to isolate phosphopeptide. The ions were introduced after the phophonation of an epoxy group on polymeric microspheres. The successful preparation of -immobilized polymeric microsphere stationary phase was confirmed through Fourier transform infrared spectra, optical microscopy, scanning electron microscopy, X-ray photoelectron spectra and inductively coupled plasma-atomic emission spectrometer. The separation efficiency for affinity column prepared by slurry packing was tested to phosphonated casein and dephosphonated casein. The resolution time (min) of the phosphonated casein was higher than that of dephosphated casein for affinity polymeric microsphere by liquid chromatography. This affinity column can be used for isolation of phosphonated casein from casein using liquid chromatography.
affinity column; poly(styrene-co-glycidyl methacrylate); polymer microsphere; phosphonated casein; resolution time;
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