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Application of Pine Peroxidase to the Amperometric Determination of Hydrogen Peroxidase
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 Title & Authors
Application of Pine Peroxidase to the Amperometric Determination of Hydrogen Peroxidase
Yoon, Kil-Joong;
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 Abstract
A pine needle-embedded graphite enzyme electrode, of which bonding agent is CSM rubber, was newly designed and its electrochemistry was studied based on the amperometry. It involved a ground green leaves of pine tree as a zymogen together with electrochemical mediator, ferrocene within the paste. The plots of ln() vs. and Lineweaver-Burk at the low potential (-100 to -500 mV) showed good linearities indicating that the amperometric response is by the catalytic power of pine peroxidase. Electrochemical parameters obtained, symmetry factor (, 0.17), limiting current (, 1.99 ), exchange current density (, ), Michaelis constant (, M) and many others showed that pine peroxidase discharges the role of catalyst quantitatively on the electrode surface. Those proved that the practical use of pine peroxidase is promising in place of the marketed.
 Keywords
Enzyme electrode;Pine peroxidase;Hydrogen peroxide;Mediator;
 Language
Korean
 Cited by
1.
CSM 고무로 결합된 담배 과산화효소 고정 효소전극의 전기화학적 특성,윤길중;

공업화학, 2014. vol.25. 5, pp.538-543 crossref(new window)
2.
고무로 결합된 과산화수소 감응 탄소반죽 전극의 빠른 감응,이범규;윤길중;

과학수사학회지, 2015. vol.9. 4, pp.250-257 crossref(new window)
1.
Electrochemical Properties of Tobacco Peroxidase Incorporated Enzyme Electrode Bound with CSM Rubber, Applied Chemistry for Engineering, 2014, 25, 5, 538  crossref(new windwow)
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