Advanced SearchSearch Tips
Analysis Method of Parabens in Human Breast Milk by LC-MS/MS System
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Analysis Method of Parabens in Human Breast Milk by LC-MS/MS System
Park, Na-Youn; Lee, Eun-Hee; Kho, Younglim;
  PDF(new window)
Parabens were commonly used for preventing the growth of microorganisms as preservatives in the pharmaceutical, cosmetic and food industry. Also, parabens are known endocrine disruptors because of their estrogenic effects on human. Parabens affect the endocrine system and show adverse effect such as, genital malformations, precocious puberty and testicular cancer in young children, infants and fetuses. In this study, we developed analytical method for four parabens (methyl paraben, ethyl paraben, propyl paraben, butyl paraben) in human breast milk which frequently consumed by newborn baby. The analytes were extracted using liquid-liquid extraction (LLE) after enzyme hydrolysis with protease and lipase, then quantitative analysis was performed by liquid chromatography tandem mass spectrometry (LC-MS/MS). The method validation results were as follows; the linearity of calibration curves were excellent with coefficient of determinations (r2) higher than 0.999, the limit of detections (LODs) were 0.019~0.044 ng/mL, the accuracies were 85.3~105.9% and the precisions were lower than 10%. The average concentration ± standard deviation of parabens in ten human breast milk sample were MP 0.660 ± 0.519 ng/mL, EP 1.631 ± 2.081 ng/mL and PP 0.326 ± 0.320 ng/mL, and BP was not detected.
Paraben;Breast milk;Liquid-liquid extraction;LC-MS/MS;
 Cited by
Schlumpf, M.; Kypke, K.; Wittassek, M.; Angerer, J.; Mascher, H.; Mascher, D.; Vökt, C.; Birchler, M.; Lichtensteiger, W. Chemosphere 2010, 81, 1171. crossref(new window)

Soni, M. G.; Carabin, I. G.; Burdock, G. A. Food and Chemical Toxicology 2005, 43, 985. crossref(new window)

Ahn, H. S.; Nah, W. H.; Lee, J. E.; Oh, Y. S.; Gye, M. C. Korean Society of Environmental Biology 2009, 27, 323.

Sabalitschka, T.; Neufeld-Crzelliter, R. Arzneimittel-Forsch 1954, 4, 575.

Taxvig, C.; Vinggaard, A. M.; Hass, U.; Axelstad, M.; Boberg, J.; Hansen, P. R.; Frederiksen, H.; Nellemann, C. Toxicological Sciences 2008, 106, 206. crossref(new window)

Darbre, P. D.; Aljarrah, A.; Miller, W. R.; Coldham, N. G.; Sauer, M. J.; Pope, G. S. Journal of Applied Toxicology 2004, 24, 5. crossref(new window)

Darbre, P. D.; Harvey, P. W. Journal of Applied Toxicology 2008, 28, 561. crossref(new window)

Bay, K.; Asklund, C.; Skakkebaek, N. E.; Andersson, A. M. Best Practice & Research Clinical Cndocrinology & Metabolism 2006, 20, 77. crossref(new window)

Golden, R.; Gandy, J.; Vollmer, G. Critical Reviews in Toxicology 2005, 35, 435. crossref(new window)

Kirchhof, M. G.; de Gannes, G. C. Skin Therapy Letter 2013, 18, 2.

Błędzka, D.; Gromadzińska, J.; Wąsowicz, W. Environment International 2014, 67, 27. crossref(new window)

Ministry of Food and Drug Safety. Korean Food Standards Codex 2015, 227.

World Health Orcanization. Fifty-fifth world health assembly 2002, A55/15.

Hines, E. P.; Mendola, P.; von Ehrenstein, O. S.; Ye, X.; Calafat, A. M.; Fenton, S. E. Reproductive toxicology 2015, 54, 120. crossref(new window)

Ye, X.; Bishop, A. M.; Needham, L. L.; Calafat, A. M. Analytica chimica acta 2008, 622, 150. crossref(new window)

Rodríguez-Gómez, R.; Roldán-Pijuán, M.; Lucena, R.; Cárdenas, S. Journal of Chromatography A 2014, 1354, 26. crossref(new window)

Wielogórska, E.; Elliott, C. T.; Danaher, M.; Chevallier, O.; Connolly, L. Food control 2015, 48, 48. crossref(new window)

Rodríguez-Gómez, R.; Dorival-García, N.; Zafra-Gómez, A.; Camino-Sánchez, F. J.; Ballesteros, O.; Navalón, A. Journal of Chromatography B 2015, 922, 47.

Sun, S. X.; Li, S. B.; Yao, P. Mechanical and Manufacturing Engineering 2015, IC3ME.

Alshana, U.; Ertas, N.; Goğer, N. G.; Food Chemistry 2015, 181, 1. crossref(new window)

Janjua, N. R.; Frederiksen, H.; Skakkebæk, N. E.; Wulf, H. C.; Andersson, A. M. International Journal of Andrology 2008, 31, 118. crossref(new window)

Lee, S. Y.; Son, E. J.; Kang, J. Y.; Lee, H. S.; Shin, M. K.; Nam, H. S.; Kim, S. Y.; Jang, Y. M.; Rhee, G. S. Bulletin of the Korean Chemical Society 2013, 34, 1131. crossref(new window)

Kim, J. H.; Kho, Y. L.; Kim, P. G.; Jeong, J. Y.; Lee, E. H.; Lee, S. Y.; Nam, H. S.; Rhee, G. S. Korean Journal of Environmental Health Sciences 2012, 38, 561. crossref(new window)

Ye, X.; Kuklenyik, Z.; Bishop, A. M.; Needham, L. L.; Calafat, A. M. Journal of Chromatography B 2006, 844, 53. crossref(new window)

Casas, L.; Fernndez, M. F.; Llop, S.; Guxens, M.; Ballester, F.; Olea, N.; Irurzun, M. B.; Rodrguez, L. S. M.; Riao, I.; Tardn, A.; Vrijheid, M.; Calafat, A. M.; Sunyer, J.; Environment International 2011, 37, 858. crossref(new window)

Liao, C.; Chen, L.; Kannan, K. Environment International 2013, 57, 68.