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Backbone assignment of the anticodon binding domain of human Glycyl-tRNA synthetase
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 Title & Authors
Backbone assignment of the anticodon binding domain of human Glycyl-tRNA synthetase
Mushtaq, Ameeq Ul; Cho, Hye Young; Byun, Youngjoo; Jeon, Young Ho;
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Backbone , and resonance assignments are presented for the anticodon binding domain (residues 557-674) of human glycyl-tRNA synthetase (GRS). Role of the anticodon binding domain (ABD) of GRS as an anticancer ligand has recently been reported and its role in other diseases like Charcot-Marie-Tooth (CMT) and polymyositis have increased its interest. NMR assignments were completed using the isotope []-enriched protein and chemical shifts based secondary structure analysis with TALOS+ demonstrate similar secondary structure as reported in X-ray structure PDB 2ZT8, except some C-terminal residues. NMR signals from the N-terminal residues 557 to 571 and 590 to 614 showed very weak or no signals exhibiting dynamics or conformational exchange in NMR timescale.
Glycyl-tRNA synthetase;anticodon binding domain(ABD);backbone assignment;
 Cited by
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