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Effects of Heavy Metal and pH on Bacterial Growth Isolated from the Contaminated Smelter Soil
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 Title & Authors
Effects of Heavy Metal and pH on Bacterial Growth Isolated from the Contaminated Smelter Soil
Keum, Mi-Jung; Yoon, Min-Ho; Nam, In-Hyun;
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The contaminated soil at abandoned smelter areas present challenge for remediation, as the degraded materials are typically deficient in nutrients, and rich in toxic heavy metals and metalloids. Bioremediation technique is to isolate new strains of microorganisms and develop successful protocols for reducing metal toxicity with heavy metal tolerant species. The present study collected metal contaminated soil and characterized for pH and EC values, and heavy metal contents. The pH value was 5.80, representing slightly acidic soil, and EC value was 13.47 mS/m. ICP-AES analytical results showed that the collected soil samples were highly contaminated with various heavy metals and metalloids such as lead (183.0 mg/kg), copper (98.6 mg/kg), zinc (91.6 mg/kg), and arsenic (48.1 mg/kg), respectively. In this study, a bacterial strain, Bacillus cereus KM-15, capable of adsorbing the heavy metals was isolated from the contaminated soils by selective enrichment and characterized to apply for the bioremediation. The effects of heavy metal on the growth of the Bacillus cereus KM-15 was determined in liquid cultures. The results showed that 100 mg/L arsenic, lead, and zinc did not affect the growth of KM-15, while the bacterial growth was strongly inhibited by copper at the same concentration. Further, the ability of the bacteria to adsorb heavy metals was evaluated.
Smelter soil;Heavy metal;Bacteria;Adsorption;Soil bioremediation;
 Cited by
Aiking, H., Govers, H, and Van’t Riet, J., 1985, Detoxification of mercury, cadmium, and lead in Klebsiella aerogenes NCTC 418 growing in continuous culture, Appl. Environ. Microbiol., 50, 1262-1267.

Amor, L., Kennes, C., and Veiga, M.C., 2001, Kinetics of inhibition in the biodegradation of monoaromatic hydrocarbons in presence of heavy metals, Bioresour. Technol., 78, 181-185. crossref(new window)

Arivalagan, P., Singaraj, D., Haridass, V., and Kaliannan, T., 2014, Removal of cadmium from aqueous solution by batch studies using Bacillus cereus, Ecol. Eng., 7, 728-735.

Baath, E., 1989, Effect of heavy metals in soil on microbial processes and population, Water Air Soil Pollut., 47, 335-379. crossref(new window)

Brown, R.A. and Cartwright, R.T., 1990, Biotreat sludge and soils, Hydrocarbon Processing, 68, 93-97.

Burgess, J.E., Quarmby, J., and Stephensen, T., 1999, Role of micronutrients in activated sludge-based biotreatment of industrial effluents, Biotechnol. Adv., 17, 49-70. crossref(new window)

Cha, M.W., Lee, H.U., and Park, J.W., 2010, A biological complex soil treatment process using selected soil bacterial strains, Kor. Geo-Environ. Society, 11, 5-13.

Chen, S.Y. and Lin, J.G., 2001, Effect of substrate concentration on bioleaching of metal-contaminated sediment, J. Hazard. Mater., 82, 77-89. crossref(new window)

Cho, J.S., Lee, W.K., Choi, H.S., and Heo, J.S., 1997, Distibution of heavy metal in the cell components of heavy metal-tolerant microorganisms, Kor. J. Enviro. Agri., 16, 55-60.

Cotter-Howells, J. and Caporn, S., 1996, Remediation of contaminated land by formation of heavy metal phosphates, Appl. Geochem., 11, 335-342. crossref(new window)

Gadd, G.M., 1992, Biosorption, J. Chem. Technol. Biotechnol., 55, 302-304.

Hong, H.B., Nam, I.H., Kim, Y.M., Chang, Y.S., and Schmidt, S., 2007, Effect of heavy metals on the biodegradation of dibenzofuran in liquid medium, J. Hazard. Mater., 140, 145-148. crossref(new window)

Hong, S.H., Koo, S.Y., Kim, S.H., Ryu, H.W., Lee, I.S., and Cho, K.S., 2010, Rhizoremediation of petroleum and heavy metal-contaminated soil using Rhizobacteria and Zea mays, Kor. J. Microbiol. Biotechnol., 38, 329-334.

Jung, M.C. and Jung, M.Y., 2006, Evaluation and management method of environmental contamination from abandoned metal mines in Korea, J. Kor. Soc. Geosystem Eng., 43, 383-394.

Kim, J., 2010, Heavy metal concentrations in soils and crops in the Poongwon mine area, J. Kor. Geoenviron. Soc., 11, 5-11.

Kim, S.U., Choi, I.W., Seo, D.C., Han, M.H., Kang, B.H., Heo, J.S., Shon, B.K., and Cho, J.S., 2005, Biosorption of heavy metal in aqueous solution by heavy metal tolerant microorganism isolated from heavy metal contaminated soil, Kor. J. Env. Agri., 24, 379-385. crossref(new window)

Korea Ministry of Environment, 2009, Regulations for Drinking Water Quality Standards and Examination, Drinking Water Policy Division, 67-90.

Lu, J., Perng, C., Lee, S., and Wan, C., 2000, Use of PCR with universal primers and restriction endonuclease digestions for detection and identification of common bacterial pathogens in cerebrospinal fluid, J. Clin. Microbiol., 38, 2076-2080.

Lee, M.S., Choi, J.D., and Chang, D.S., 1983, Effects of pH, sodium chloride and potassium sorbate on the germination of Bacillus cereus spores in cooked rice homogenate, Kor. Fish. Soc., 16, 37-43.

Lee, S.S., Kim, S.M., Park, U.Y., Kim, H.Y., and Shin, I.S., 2002, Studies on proteolytic and fibrinolytic activity of Bacillus subtilis JM-3 isolated from Anchovy Sauce, Kor. J. Food Sci. Technol., 34, 283-289.

Mittal, S.K. and Ratra, R.K., 2000, Toxic effect of metal ions on biochemical oxygen demand, Water Res., 34, 147-152. crossref(new window)

Mousavi, S.M., Yaghmaei, S., Vossoughi, M., Jafari, A., and Hoseini, S.A., 2005, Comparison of bioleaching ability of two native mesophilic and thermophilic bacteria on copper recovery from chalcopyrite concentrate in an airlift bioreactor, Hydrometallurgy, 80, 139-144. crossref(new window)

Mullen, M.D., Wolf, D.C., Ferris, F.G., Beveridge, T.J., Flemming, C.A., and Bailey, G.W., 1989, Bacterial sorption of heavy metals, Appl. Environ, Microbiol., 55, 3143-3149.

Nam, I.H., Kim, J.G., and Chon, C.M., 2012, Heavy metal effects on the biodegradation of fluorene by Sphingobacterium sp. KM-02 in liquid medium, J. Soil Groundw. Environ., 17, 74-81.

Nies, D.H., 1999, Microbial heavy-metal resistance, Appl. Microbiol. Biotechnol., 51, 730-750. crossref(new window)

Pepi, M., Volterrani, M., Renzi, M., Marvasi, M., Gasperini, S., Franchi, E., and Focardi, S.E., 2007, Arsenic-resistant bacteria isolated from contaminated sediments of the Orbetello Lagoon, Italy, and their characterization, J. Appl. Microbiol., 103, 2299-2308. crossref(new window)

Riis, V., Babel, W., and Pucci, O.H., 2002, Influence of heavy metals on the microbial degradation of diesel fuel, Chemosphere, 49, 559-568. crossref(new window)

Said, W.A. and Lewis, D.L., 1991, Quantitative assessment of the effects of metals on microbial degradation of organic chemicals, Appl. Environ. Microbiol., 57, 1498-1503.

Seon, Y.H., 2009, The effect of microorganisms, nutrients, and surfactants on the bioremediation of oil-contaminated soil, Kor. J. Biotechnol. Bioengineer., 24, 53-58.

Sokhn, J., De Leij, F.A., Hart, T.D., and Lynch, J.M., 2001, Effect of copper on the degradation of phenanthrene by soil microorganisms, Lett. Appl. Microbiol., 33, 164-168. crossref(new window)

Tyler, G., 1974, Heavy metal pollution and soil enzymatic activity, Plant Soil, 41, 303-311. crossref(new window)

Vannini, C., Rosati, G., Verni, F., and Petroni, G., 2004, Identification of the bacterial endocymbionts of the marine ciliate Euplotes magnicirratus (Ciliophora, Hypotrichia) and proposal of ‘Candidatus Devosia euplotis’, Int. J. Syst. Evol. Microbiol, 54, 1151-1156. crossref(new window)

Volesky, B. and Holan, Z.R., 1995, Biosorption of heavy metals, Biotechnol. Prog., 11, 235-250. crossref(new window)

Xie, X., Fu, J., Wang, H., and Liu, J., 2010, Heavy metal resistance by two bacteria strains isolated from a copper mine tailing in China, African J. Biotechnol., 9, 4056-4066.

Yang, J.W. and Lee Y.J., 2007, Status of soil remediation and technology development in Korea, Kor. Chem. Eng. Res., 45, 311-318.