JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Changes in the Neurogenesis and Axonal Sprouting in the Organotypic Hippocampal Slice Culture by Aβ25-35 Treatment
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Applied Microscopy
  • Volume 42, Issue 4,  2012, pp.200-206
  • Publisher : Korean Society of Electron Microscopy
  • DOI : 10.9729/AM.2012.42.4.200
 Title & Authors
Changes in the Neurogenesis and Axonal Sprouting in the Organotypic Hippocampal Slice Culture by Aβ25-35 Treatment
Jung, Yeon Joo; Jiang, Hui Ling; Lee, Kyung Eun;
  PDF(new window)
 Abstract
Induction of neurogenesis can occur in the hippocampus in response to various pathological conditions, such as Alzheimer's disease. The aim of this study was to investigate the changes that occur in endogenous neural stem cells in response to amyloid beta -induced neuronal cell damage in organotypic hippocampal slice cultures. Cresyl violet staining and Fluoro-Jade B staining were used to detect neuronal cell damage and changes of mossy fiber terminals were observed by Timm's staining. The immunofl uorescence staining was used to detect the newly generated cells in the subgranular zone (SGZ) of the dentate gyrus with specific marker, 5-bromo-2'-deoxyuridine (BrdU), Ki-67, Nestin, and doublecortin (DCX). In compared to control slices, neuronal cell damage was observed and the mossy fibers were expanded to CA3 area by treatment with . Ki-67/Nestin- and BrdU/DCX-positive cells were detected in the SGZ. In conclusion, these results demonstrate that -induced neuronal damage results in an increase in endogenous neural stem cells in rat hippocampal slice cultures not only for gliosis but also for neurogenesis.
 Keywords
Neurogenesis;-induced neuronal cell damage;Subgranular zone;Dentate gyrus;Mossy fiber terminals;
 Language
English
 Cited by
 References
1.
Bjorklund N L, Reese L C, Sadagoparamanujam V M, Ghirardi V, Woltjer R L, and Taglialatela G (2012) Absence of amyloid beta oligomers at the postsynapse and regulated synaptic Zn2+ in cognitively intact aged individuals with Alzheimer's disease neuropathology. Mol. Neurodegener 7, 23. crossref(new window)

2.
Bunk E C, Konig H G, Bonner H P, Kirby B P, and Prehn J H (2010) NMDA-induced injury of mouse organotypic hippocampal slice cultures triggers delayed neuroblast proliferation in the dentate gyrus: an in vitro model for the study of neural precursor cell proliferation. Brain Res. 1359, 22-32. crossref(new window)

3.
Bush A I, Pettingell W H, Multhaup G, d Paradis M, Vonsattel J P, Gusella J F, Beyreuther K, Masters C L, and Tanzi R E (1994) Rapid induction of Alzheimer A beta amyloid formation by zinc. Science 265, 1464- 1467. crossref(new window)

4.
Chechneva O, Dinkel K, Schrader D, and Reymann K G (2005) Identification and characterization of two neurogenic zones in interface organotypic hippocampal slice cultures. Neuroscience 136, 343-355. crossref(new window)

5.
Chuang T T (2010) Neurogenesis in mouse models of Alzheimer's disease. BBA 1802, 872-880. crossref(new window)

6.
Dehghani F, Hischebeth G T, Wirjatijasa F, Kohl A, Korf H W, and Hailer N P (2003) The immunosuppressant mycophenolate mofetil attenuates neuronal damage after excitotoxic injury in hippocampal slice cultures. Eur. J. Neurosci. 18, 1061-1072. crossref(new window)

7.
Deshpande A, Kawai H, Metherate R, Glabe C G, and Busciglio J (2009) A role for synaptic zinc in activity-dependent Abeta oligomer formation and accumulation at excitatory synapses. J. neurosci. 29, 4004- 4015. crossref(new window)

8.
Eriksson P S, Perfilieva E, Bjork-Eriksson T, Alborn A M, Nordborg C, Peterson D A, and Gage F H (1998) Neurogenesis in the adult human hippocampus. Nat. Med. 4, 1313-1317. crossref(new window)

9.
Faller P (2009) Copper and zinc binding to amyloid-beta: coordination, dynamics, aggregation, reactivity and metal-ion transfer. Chembiochem: Eur. J. Chem. Biol. 10, 2837-2845. crossref(new window)

10.
Haughey N J, Liu D, Nath A, Borchard A C, and Mattson M P (2002) Disruption of neurogenesis in the subventricular zone of adult mice, and in human cortical neuronal precursor cells in culture, by amyloid beta-peptide: implications for the pathogenesis of Alzheimer's disease. Neuromolecular Med. 1, 125-135. crossref(new window)

11.
Haydar T F, Bambrick L L, Krueger B K, and Rakic P (1999) Organotypic slice cultures for analysis of proliferation, cell death, and migration in the embryonic neocortex. Brain Res. 4, 425-437.

12.
Heo C, Chang K A, Choi H S, Kim H S, Kim S, Liew H, Kim J A, Yu E, Ma J, and Suh Y H (2007) Effects of the monomeric, oligomeric, and fi brillar Abeta42 peptides on the proliferation and differentiation of adult neural stem cells from subventricular zone. J. Neurochem. 102, 493-500. crossref(new window)

13.
Huang E P (1997) Metal ions and synaptic transmission: think zinc. Proc. Natl. Acad. Sci. U S A 94, 13386-13387. crossref(new window)

14.
Kuan C Y, Schloemer A J, Lu A, Burns K A, Weng W L, Williams M T, Strauss K I, Vorhees C V, Flavell R A, Davis R J, Sharp F R, and Rakic P (2004) Hypoxia-ischemia induces DNA synthesis without cell proliferation in dying neurons in adult rodent brain. J. Neurosci. 24, 10763-10772. crossref(new window)

15.
Lie D C, Song H, Colamarino S A, Ming G L, and Gage F H (2004) Neurogenesis in the adult brain: new strategies for central nervous system diseases. Ann. Rev. Pharm. Toxicol. 44, 399-421. crossref(new window)

16.
Lopez-Toledano M A and Shelanski M L (2004) Neurogenic effect of betaamyloid peptide in the development of neural stem cells. J. Neurosci. 24, 5439-5444. crossref(new window)

17.
Lopez-Toledano M A and Shelanski M L (2007) Increased neurogenesis in young transgenic mice overexpressing human APP(Sw, Ind). J. Alzheimer's Dis. 12, 229-240. crossref(new window)

18.
Mattson M P (2000) Apoptosis in neurodegenerative disorders. Nat. Rev. Mol. Cell Bio. 1, 120-129. crossref(new window)

19.
Mazur-Kolecka B, Golabek A, Nowicki K, Flory M, and Frackowiak J (2006) Amyloid-beta impairs development of neuronal progenitor cells by oxidative mechanisms. Neurobiol. Aging 27, 1181-1192. crossref(new window)

20.
Namba T, Mochizuki H, Onodera M, Namiki H, and Seki T (2007) Postnatal neurogenesis in hippocampal slice cultures: early in vitro labeling of neural precursor cells leads to efficient neuronal production. J. Neurosci. Res. 85, 1704-1712. crossref(new window)

21.
Perez-Gomez A and Tasker R A (2012) Enhanced neurogenesis in organotypic cultures of rat hippocampus after transient subfieldselective excitotoxic insult induced by domoic acid. Neuroscience 208, 97-108. crossref(new window)

22.
Picard-Riera N, Nait-Oumesmar B, and Baron-Van Evercooren A (2004) Endogenous adult neural stem cells: limits and potential to repair the injured central nervous system. J. Neurosci. Res. 76, 223-231. crossref(new window)

23.
Plumpe T, Ehninger D, Steiner B, Klempin F, Jessberger S, Brandt M, Romer B, Rodriguez G R, Kronenberg G, and Kempermann G (2006) Variability of doublecortin-associated dendrite maturation in adult hippocampal neurogenesis is independent of the regulation of precursor cell proliferation. BMC Neurosci. 7, 77. crossref(new window)

24.
Raineteau O, Rietschin L, Gradwohl G, Guillemot F, and Gahwiler BH (2004) Neurogenesis in hippocampal slice cultures. Mol. Cell. Neurosci. 26, 241-250. crossref(new window)

25.
Religa D, Strozyk D, Cherny R A, Volitakis I, Haroutunian V, Winblad B, Naslund J, and Bush A I (2006) Elevated cortical zinc in Alzheimer disease. Neurology 67, 69-75. crossref(new window)

26.
Rodriguez J J, Jones V C, and Verkhratsky A (2009) Impaired cell proliferation in the subventricular zone in an Alzheimer's disease model. Neuroreport 20, 907-912. crossref(new window)

27.
Rola R, Raber J, Rizk A, Otsuka S, VandenBerg S R, Morhardt D R, and Fike J R (2004) Radiation-induced impairment of hippocampal neurogenesis is associated with cognitive defi cits in young mice. Exp. Neurol. 188, 316-330. crossref(new window)

28.
Sadgrove M P, Laskowski A, and Gray W P (2006) Examination of granule layer cell count, cell density, and single-pulse BrdU incorporation in rat organotypic hippocampal slice cultures with respect to culture medium, septotemporal position, and time in vitro. J. Comp. Neurol. 497, 397-415. crossref(new window)

29.
Scheffl er B, Schmandt T, Schroder W, Steinfarz B, Husseini L, Wellmer J, Seifert G, Karram K, Beck H, Blumcke I, Wiestler O D, Steinhauser C, and Brustle O (2003) Functional network integration of embryonic stem cell-derived astrocytes in hippocampal slice cultures. Development (Cambridge, England) 130, 5533-5541. crossref(new window)

30.
Schaffer D V and Gage F H (2004) Neurogenesis and neuroadaptation. Neuromolecular Med. 5, 1-9. crossref(new window)

31.
Suh E C, Jung Y J, Kim Y A, Park E M, and Lee K E (2008) A beta 25- 35 induces presynaptic changes in organotypic hippocampal slice cultures. Neurotoxicology 29, 691-699. crossref(new window)

32.
Tamura M, Koyama R, Ikegaya Y, Matsuki N, and Yamada M K (2006) K252a, an inhibitor of Trk, disturbs pathfinding of hippocampal mossy fibers. Neuroreport 17, 481-486. crossref(new window)

33.
Tanaka A, Watanabe Y, Kato H, and Araki T (2007) Immunohistochemical changes related to ageing in the mouse hippocampus and subventricular zone. Mech. Ageing Dev. 128, 303-310. crossref(new window)

34.
Tanvig M, Blaabjerg M, Andersen R K, Villa A, Rosager A M, Poulsen F R, Martinez-Serrano A, Zimmer J, and Meyer M (2009) A brain slice culture model for studies of endogenous and exogenous precursor cell migration in the rostral migratory stream. Brain Res. 1295, 1-12. crossref(new window)

35.
Wang J M, Irwin R W, Liu L, Chen S, and Brinton R D (2007) Regeneration in a degenerating brain: potential of allopregnanolone as a neuroregenerative agent. Curr. Alzheimer Res. 4, 510-517. crossref(new window)

36.
Wang S H, Zhang Z J, Guo Y J, Teng G J, and Chen B A (2008) Hippocampal neurogenesis and behavioural studies on adult ischemic rat response to chronic mild stress. Behav. Brain Res. 189, 9-16. crossref(new window)

37.
Zhang C, McNeil E, Dressler L, and Siman R (2007) Long-lasting impairment in hippocampal neurogenesis associated with amyloid deposition in a knock-in mouse model of familial Alzheimer's disease. Exp. Neurol. 204, 77-87. crossref(new window)

38.
Ziabreva I, Perry E, Perry R, Minger S L, Ekonomou A, Przyborski S, and Ballard C (2006) Altered neurogenesis in Alzheimer's disease. J. Psychosom. Res. 61, 311-316. crossref(new window)