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The Localization of Cytokeratin 19 and Vimentin in Sprague Dawley Albino Rat Skin Tissue
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  • Journal title : Applied Microscopy
  • Volume 44, Issue 1,  2014, pp.15-20
  • Publisher : Korean Society of Electron Microscopy
  • DOI : 10.9729/AM.2014.44.1.15
 Title & Authors
The Localization of Cytokeratin 19 and Vimentin in Sprague Dawley Albino Rat Skin Tissue
Kim, Tae Keun; Kim, Yong Joo; Min, Byoung Hoon; Kim, Soo Jin;
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 Abstract
Cytokeratin 19 (CK19) expressed in epidermis of skin, bulge region of hair follicle, outermost layer of outer root sheath and proximal and distal to bulge. Vimentin is a fibrous protein that localized in cytoplasm of fibroblast and forms cytoskeleton to maintain shape of cell and nucleus. In this study, CK19 and vimentin in skin were confirmed with light, fluorescence and transmission electron microscope. As a result, CK19 was localized epidermis, hair follicles, outer root sheath and nucleus of Merkel's cell. However, vimentin was localized some epidermis, dermis, hypodermis and nucleus of Merkel's cell. The role of CK19 is self-renewal and homeostasis in skin. Also, hair follicle regeneration and hair growth is known to be related. It is supposed that required of structural proteins that make up cytoskeleton is increased. Thereby, expression of CK19 is increased. It is considered that vimentin localized in order to stabilize structure of cell and cytoskeleton of fibroblasts. Also, CK19 and vimentin present in nuclei of Merkel's cell, and to act as a fibrous protein that make up end of a nerve fiber present in Merkel's cell and paracrine function of Merkel's cell.
 Keywords
Cytokeratin 19;Vimentin;Skin;
 Language
English
 Cited by
 References
1.
Abbas O and Mahalingam M (2009) Epidermal stem cells: practical perspectives and potential uses. Br. J. Dermatol. 161, 228-236. crossref(new window)

2.
Abbas O, Richards J E, Yaar R, and Mahalingam M (2011) Stem cell markers (cytokeratin 15, cytokeratin 19 and p63) in in situ and invasive cutaneous epithelial lesions. Modern Pathology 24, 90-97. crossref(new window)

3.
Bartek J, Taylor-Papadimitriou J, Miller N, and Millis R (1985) Patterns of expression of keratin 19 as detected with monoclonal antibodies in human breast tissues and tumours. Int. J. Cancer 36, 299-306.

4.
Fradette J, Godbout M J, Michel M, and Germain L (1995) Localization of Merkel cells at hairless and hairy human skin sites using keratin 18. Biochem. Cell Biol. 73, 635-639. crossref(new window)

5.
Goldman R D, Khuon S, Chou Y H, Opal P, and Steinert P M (1996) The function of intermediate filaments in cell shape and cytoskeletal integrity. J. Cell Biol. 134, 971-983. crossref(new window)

6.
Goldman R D and Knipe D M (1973) Functions of cytoplasmic fibers in non-muscle cell motility. Cold Spring Harbor Symp. Quant. Biol. 37, 523-534. crossref(new window)

7.
Gonzales M, Weksler B, Tsuruta D, Goldman R D, Yoon K J, and Hopkinson S B (2001) Structure and function of a vimentinassociated matrix adhesion in endothelial cells. Mol. Biol. Cell 12, 85-100. crossref(new window)

8.
Hall P A and Watt F M (1989) Stem cells: the generation and maintenance of cellular diversity. Development 106, 619-633.

9.
Heid H W, Moll I, and Franke W W (1988) Patterns of expression of trichocytic and epithelial cytokeratins in mammalian tissues. I. Human and bovine hair follicles. Differentiation 37, 137-157. crossref(new window)

10.
Herrmann H, Bar H, Kreplak L, Strelkov S V, and Aebi U (2007) Intermediate filaments: from cell architecture to nanomechanics. Nat. Rev. Mol. Cell Biol. 8, 562-573. crossref(new window)

11.
Kirfel J, Magin T M, and Reichelt J (2003) Keratins: a structural scaffold with emerging functions. Cell Mol. Life Sci. 60, 56-71. crossref(new window)

12.
Kloepper J E, Tiede S, and Brinckmann J (2008) Immunophenotyping of the human bulge region: the quest to define useful in situ markers for human epithelial hair follicle stem cells and their niche. Exp. Dermatol. 17, 592-609. crossref(new window)

13.
Lajtha L G (1979) Stem cell concepts. Differentiation 14, 23-34. crossref(new window)

14.
Lammerding J, Hsiao J, Schulze PC, Kozlov S, Stewart C L, and Lee R T (2005) Abnormal nuclear shape and impaired mechanotransduction in emerindeficient cells. J. Cell Biol. 170, 781-791. crossref(new window)

15.
Lavker R M and Sun T T (1982) Heterogeneity in epidermal basal keratinocytes: morphological and functional correlations. Science 215, 1239-1241. crossref(new window)

16.
Lazarides E (1980) Intermediate filaments as mechanical integrators of cellular space. Nature 283, 249-256. crossref(new window)

17.
Leblond C P (1981) The life history of cells in renewing systems. Am. J. Anat. 160, 114-157.

18.
Lyle S, Christofidou-Solomidou M, and Liu Y (1998) The C8/144B monoclonal antibody recognizes cytokeratin 15 and defines the location of human hair follicle stem cells. J. Cell Sci. 111, 3179-3188.

19.
Lyle S, Christofidou-Solomidou M, and Liu Y (1999) Human hair follicle bulge cells are biochemically distinct and possess an epithelial stem cell phenotype. J. Investig. Dermatol. Symp. Proc. 4, 296-301. crossref(new window)

20.
Mendez M G, Kojima S I, and Goldman R D (2010) Vimentin induces changes in cell shape, motility, and adhesion during the epithelial to mesenchymal transition. FASEB J. 24, 1838-1851. crossref(new window)

21.
Michel M, Torok N, Godbout M J, Lussier M, Gaudreau P, Royal A, and Germain L (1996) Keratin 19 as a biochemical marker of skin stem cells in vivo and in vitro: keratin 19 expressing cells are differentially localized in function of anatomic sites, and their number varies with donor age and culture stage. J. Cell Sci. 109(Pt 5), 1017-1028.

22.
Moll I (1994) Merkel cell distribution in human hair follicles of the fetal and adult scalp. Cell Tissue Res. 277, 131-138. crossref(new window)

23.
Moll R, Franke W W, Schiller D L, Geiger B, and Krepler R (1982) The catalog of human cytokeratins: patterns of expression in normal epithelial, tumors and cultured cells. Cell 31, 11-24. crossref(new window)

24.
Mucke N, Wedig T, Burer A, Marekov L N, Steinert P M, and Langowski J (2004) Molecular and biophysical characterization of assemblystarter units of human vimentin. J. Mol. Biol. 340, 97-114. crossref(new window)

25.
Nadia N, Nadeem R, Nagi A H, Ashraf M, and Waqas S (2010) Immunohistochemical expression of cytokeratin-19 in non small cell lung carcinomas-an experience from a tertiary care hospital in Lahore. Int. J. Pathol. 8, 54-59.

26.
Potten C S and Loeffler M (1990) Stem cells: attributes, cycles, spirals, pitfalls and uncertainties. Lessons for and from the Crypt. Development 110, 1001-1020.

27.
Stasiak P C, Purkis P E, Leigh I M, and Lane E B (1989) Keratin 19: predicted amino acid sequence and broad tissue distribution suggest it evolved from keratinocyte keratins. J. Invest. Dermatol. 92, 707-716. crossref(new window)

28.
Steinert P M and Roop D R (1988) Molecular and cellular biology of intermediate filaments. Annu. Rev. Biochem. 57, 593-625. crossref(new window)

29.
Su L, Morgan P R, Thomas J A, and Lane E B (1993) Expression of keratin 14 and 19 mRNA and protein in normal oral epithelia, hairy leukoplakia, tongue biting and white sponge nevus. J. Oral. Pathol. Med. 22, 183-189. crossref(new window)

30.
Tolstonog G V, Belichenko-Weitzmann I V, Lu J P, Hartig R, Shoeman R L, Traub U, and Traub P (2005) Spontaneously immortalizedmouse embr yo f ibroblasts: growth behavior of wild-type and vimentindeficient cells in relation to mitochondrial structure and activity. DNA Cell Biol. 24, 680-709. crossref(new window)

31.
Wang N, Butler J P, and Ingber D E (1993) Mechanotransduction across the cell-surface and through the cytoskeleton. Science 260, 1124-1127. crossref(new window)