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Expression of Thymosin β4 in Ameloblasts during Mouse Tooth Development
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  • Journal title : Applied Microscopy
  • Volume 46, Issue 1,  2016, pp.58-66
  • Publisher : Korean Society of Electron Microscopy
  • DOI : 10.9729/AM.2016.46.1.58
 Title & Authors
Expression of Thymosin β4 in Ameloblasts during Mouse Tooth Development
Choi, Baik-Dong; Lee, Seung-Yeon; Nho, Tae-Hee; Jeong, Soon-Jeong; Lim, Do-Seon; Bae, Chun-Sik; Jeong, Moon-Jin;
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 Abstract
Thymosin () has been recently reported to play a role in dentinogenesis by regulating the expression of dentin matrix proteins. Based on previous studies, it is hypothesized that is associated with the formation of the enamel matrix and thus plays an important role in ameloblast. However, there is no report on the function of during tooth development so far. Therefore, in this study, we aimed to investigate the expression of and its function in ameloblasts during mouse tooth development. was expressed strongly in the tooth bud at the bud stage and in the dental lamina and oral epithelium at the cap stage. In advanced bell stage at postnatal day 4, large elongated ameloblasts were observed and the expression of the protein was the highest, with the enamel being was thicker than that in the early bell stage. The length of ameloblasts increased from the presecretory to the secretory stage and decreased from the maturation to the protective stage. These results suggest that participates not only in the proliferation of oral epithelial cells during the early stage of tooth development but also regulates enamel protein secretion in ameloblasts and enamel mineralization.
 Keywords
Thymosin b4;Ameloblast;Amelogenesis;Tooth development;Mouse;
 Language
English
 Cited by
 References
1.
Akhter M, Kobayashi I, Kiyoshima T, Matsuo K, Yamaza H, Wada H, Honda J Y, Ming X, and Sakai H (2005) Possible functional involvement of thymosin beta 4 in developing tooth germ of mouse lower first molar. Histochem. Cell Biol. 124, 207-213. crossref(new window)

2.
Anadon R, Rodriguez Moldes I, Carpintero P, Evangelatos G, Livianou E, Leondiadis L, Quintela I, Cervino M C, and Gomez-Marquez J (2001) Differential expression of thymosins beta(4) and beta(10) during rat cerebellum postnatal development. Brain Res. 894, 255-265. crossref(new window)

3.
Boyde A (1989) Enamel. In: Handbook of Microscopic Anatomy, eds. Oksche A and Vollrath L, pp. 309-473, (Springer, Berlin).

4.
Caton J, Bringas P Jr, and Zeichner-David M (2005) IGFs increase enamel formation by inducing expression of enamel mineralizing specific genes. Arch. Oral Biol. 50, 123-129. crossref(new window)

5.
Cha H J, Philp D, Lee S H, Moon H S, Kleinman H K, and Nakamura T (2010) Over-expression of thymosin beta 4 promotes abnormal tooth development and stimulation of hair growth. Int. J. Dev. Biol. 54, 135-140. crossref(new window)

6.
Cho A, Haruyama N, Hall B, Danton M J, Zhang L, Arany P, Mooney D J, Harichane Y, Goldberg M, Gibson C W, and Kulkarni A B (2013) TGF-${\beta}$ regulates enamel mineralization and maturation through KLK4 expression. PLoS One 8, e82267. crossref(new window)

7.
Choi B D, Jeong S J, Lee H Y, Lim D S, Lee B H, Bae C S, and Jeong M J (2015) The effect of Thymosin ${\beta}4$ for osteoblast adhesion on titanium surface. J. Nanosci. Nanotechnol. 15, 5663-5667. crossref(new window)

8.
Choi B D, Yun S H, Jeong S J, Wang G, Kim H J, Lim D S, and Jeong M J (2012) Expression of thymosin ${\beta}4$ in odontoblasts during mouse tooth development. Int. J. Mol. Med. 29, 841-847.

9.
Fincham A G, Moradian-Oldak J, and Simmer J P (1999) The structural biology of the developing dental enamel matrix. J. Struct. Biol. 126, 270-299. crossref(new window)

10.
Fukumoto S, Kiba T, Hall B, Iehara N, Nakamura T, Longenecker G, Krebsbach P H, Nanci A, Kulkarni A B, and Yamana Y (2004) Ameloblastin regulates cell adhesion and differentiation states of dental epithelium and is essential for amelogenesis. J. Cell Biol. 167, 973-983. crossref(new window)

11.
Fukumoto S, Yamada A, Nonaka K, and Yamada Y (2005) Essential roles of ameloblastin in maintaining ameloblast differentiation and enamel formation. Cells Tissues Organs 181, 189-195.

12.
Gibson C W, Yuan Z A, Hall B, Longenecker G, Chen E, Thyagarajan T, Sreenath T, Wright J T, Decker S, Piddington R, Harrison G, and Kulkarni A B (2001) Amelogenin-deficient mice display an amelogenesis imperfecta phenotype. J. Biol. Chem. 276, 31871-31875. crossref(new window)

13.
Goldstein A L, Hannappel E, and Kleinman H K (2005) Thymosin beta4: actin-sequestering protein moonlights to repair injured tissues. Trends Mol. Med. 11, 421-429. crossref(new window)

14.
Gomez-Marquez J, Franco del Amo F, Carpintero P, and Anadon R (1996) High levels of mouse thymosin beta4 mRNA in differentiating P19 embryonic cells and during development of cardiovascular tissues. Biochim. Biophys. Acta 1306, 187-193. crossref(new window)

15.
Grant D S, Rose W, Yaen C, Goldstein A, Martinez J, and Kleinman H (1999) Thymosin beta4 enhances endothelial cell differentiation and angiogenesis. Angiogenesis 3, 125-135. crossref(new window)

16.
Hillson S (1996) Dental enamel. In: Dental Anthropology, ed. Hillson S, pp. 148-181, (Cambridge University Press, Cambridge).

17.
Jeong J O, Wang G, Jeong S J, Choi B D, Lee H Y, and Jeong M J (2015) Function of secretory leukocyte protease inhibitor (SLPI) in odontoblast during mouse tooth development. J. Nanosci. Nanotechnol. 15, 120-124. crossref(new window)

18.
Kobayashi I, Kiyoshima T, Wada H, Matsuo K, Nonaka K, Honda J Y, Koyano K, and Sakai H (2006) Type II/III Runx2/Cbfa1 is required for tooth germ development. Bone 38, 836-844. crossref(new window)

19.
Lacruz R S, Nanci A, Kurtz I, Wright J T, and Paine M L (2010) Regulation of pH during amelogenesis. Calcif. Tissue Int. 86, 91-103. crossref(new window)

20.
Lee S I, Kim D S, Lee H J, Cha H J, and Kim E C (2013) The role of thymosin beta 4 on odontogenic differentiation in human dental pulp cells. PLoS One 8, e61960. crossref(new window)

21.
Moradian-Oldak J (2009) The regeneration of tooth enamel. Dimens. Dent. Hyg. 7, 12-15.

22.
Moradian-Oldak J (2012) Protein-mediated enamel mineralization. Front. Biosci. (Landmark Ed) 17, 1996-2023. crossref(new window)

23.
Nanci A (2008) Enamel: composition, formation, and structure. In: Ten Cate's Oral Histology, Developmrent, Structure, and Function, ed. Nanci A, pp. 141-190, (Mosbey Elsevier, St. Louis).

24.
Ookuma Y F, Kiyoshima T, Kobayashi I, Nagata K, Wada H, Fujiwara H, Yamaza H, Nonaka K, and Sakai H (2013) Multiple functional involvement of thymosin beta-4 in tooth germ development. Histochem. Cell Biol. 139, 355-370. crossref(new window)

25.
Paine M L, Wang H J, Luo W, Krebsbach P H, and Snead M L (2003) A transgenic animal model resembling amelogenesis imperfecta related to ameloblastin overexpression. J. Biol. Chem. 278, 19447-19452. crossref(new window)

26.
Philp D, St-Surin S, Cha H J, Moon H S, Kleinman H K, and Elkin M (2007) Thymosin beta 4 induces hair growth via stem cell migration and differentiation. Ann. NY Acad. Sci. 1112, 95-103. crossref(new window)

27.
Rajpar M H, Harley K, Laing C, Davies R M, and Dixon M J (2001) Mutation of the gene encoding the enamel-specific protein, enamelin, causes autosomal-dominant amelogenesis imperfecta. Hum. Mol. Genet. 10, 1673-1677. crossref(new window)

28.
Safer D, Sosnick T R, and Elzinga M (1997) Thymosin beta 4 binds actin in an extended conformation and contacts both the barbed and pointed ends. Biochemistry 36, 5806-5816. crossref(new window)

29.
Yamaza H, Matsuo K, Kiyoshima T, Shigemura N, Kobayashi I, Wada H, Akamime A, and Sakai H (2001) Detection of differentially expressed genes in the early developmental stage of the mouse mandible. Int. J. Dev. Biol. 45, 675-680.