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HPLC/MS/MS Method for Determination of Soyasaponins in the Soybean Varieties
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  • Journal title : KOREAN JOURNAL OF CROP SCIENCE
  • Volume 56, Issue 3,  2011, pp.244-249
  • Publisher : The Korean Society of Crop Science
  • DOI : 10.7740/kjcs.2011.56.3.244
 Title & Authors
HPLC/MS/MS Method for Determination of Soyasaponins in the Soybean Varieties
Han, Sang-Jun;
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 Abstract
A sensitive and rapid high-performance liquid chromatographic-tandem mass spectrometric (HPLC/MS/MS) assay was developed for the determination of soyasaponins in soybean. Among soyasaponins, soyasaponin I was isolated and characterized from methanol extracts of soybean as analytical standards and the development of a new analytical procedure for quantification of its content in various cultivars. The structures of these compound was elucidated by , NMR experiments and by mass spectrometric analysis. Aqueous ethanol extracts of soybean samples were injected on an Agilent XDB-C18 column (, ) with a mobile phase consisting of 10 mM ammonium acetate-acetonitrile, a flow rate of 0.3 mL/min and a total run time of 8 min. Detection was performed by mass spectrometer bin the multiple reaction monitoring (MRM) mode with negative electrospray ionization (ESI) m/z at 941 615 for soyasaponin I. In the 9 soybean samples, contents of soyasaponin I ranged from 205 to 726 mg/kg, and correlated negatively with seed size.
 Keywords
soybean;soyasaponin;HPLC/MS/MS;QQQ;
 Language
Korean
 Cited by
1.
미국으로부터 재도입된 한국 원산 콩 재래종의 Saponin 함량 변이,장은규;박향민;황태영;김선림;정규화;;최유미;이명철;이정란;김홍식;

한국작물학회지, 2012. vol.57. 3, pp.286-295 crossref(new window)
1.
Changes of Soyasaponin Contents in Soybean Sprouts, The Korean Journal of Crop Science, 2016, 61, 1, 57  crossref(new windwow)
2.
Variation of Saponin Content in Korean Native Soybean Landraces Reintroduced from USA to Korea, Korean Journal of Crop Science, 2012, 57, 3, 286  crossref(new windwow)
 References
1.
Adlercreutz, H. and W. Mazur. 1997. Phyto-estrogens and western diseases. Ann. Med. 29 : 95-120. crossref(new window)

2.
Chung, H. J., Q. Liu, R. Hoover, T. D. Warkentin, B. Vandenberg. 2008. In vitro starch digestibility, expected glycemic index, and thermal and pasting properties of flours from pea, lentil and chickpea cultivars. J. Food Chem. 118 : 316-321.

3.
Daveby, Y. D., P. Aman, J. M. Betz, and S. M. Musser. 1998. Effect of storage and extraction on ration of soyasaponin I to 2,3-Dihydro-2,5-dihydroxy-6-methyl-4-pyrone-conjugated soyasaponin I in dehulled peas (Pisum sativum L). J. Sci. Food Agric. 141-146.

4.
Kang, E. Y., S. H. Kim, S. L. Kim, S. H. Seo, E. H. Kim, H. K. Song, J. K. Ahn, and I. M. Chung. 2010. Comparison of soyasapogenol A, B concentrations in soybean seeds and sprouts. Korean J. Crop Sci. 55(2) : 165-176.

5.
Kim, S. L., M. A. Berhow, J. T. Kim, I. M. Chung, H. Y. Chi, J. Song, N. K. Park, and J. R. Son. 2006. Composition and content of soyasaponins and their interaction with chemical components in different seed-size soybeans. Korean J. Crop Sci. 51(4) : 340-347.

6.
Kim, Y. H. 2003. Biological activities of soyasaponins and their genetic and environmental variations in soybean. Korean J. Crop Sci. 48(S) : 49-57.

7.
Lampe, J. W. 2003. isoflavonoid and lignan phytoestrogens as dietary biomarkers. J. Nutr. 133(suppl 3) : 956S-964S. crossref(new window)

8.
Lee, H. P., L. Gourley, S. W. Duffy, J. Esteve, and J. Lee. 1991. Day NE: Dietary effects on breast-cancer risk in Singapore. Lancet. 337(8751) : 1197-1200. crossref(new window)

9.
Perterson, G. 1995. Evaluation of the biochemical targets of genistein in tumor cells. J. Nur. 125(3 Suppl) : 784-789.

10.
Ruiz, R. G., K. R. Price, A. E. Arthur, M. E. Rose, M. J. C. Rhodes, R. G. Fenwick. 1996. Effect of soaking and cooking on the saponin content and composition of chickpeas (Cicer arietinum) and lentils (Lens culinaris). J. Agric. Food Chem. 44 : 1526-1530. crossref(new window)

11.
Sakamoto, S., S Kofuji, M. Kuroyanagi, A. Ueno, and S. Sekita. 1992. Saponins from Trifolium repens. Phytochemistry. 31(5) : 1773-1777. crossref(new window)

12.
Song, T. T., S. Hendrich, and P. A. Murphy. 1999. Estrogenic activity of glycitein, a soy isoflavone. J. Agric Food Chem. 47(4) : 1607-1610. crossref(new window)

13.
Tham, D. M., C. D. Gardner, and W. L. Haskell. 1998. Potential health benefits of dietary phytoestrogens: a review of clinical, epidemiological, and mechanistic evidence. J. Clin. Endocrinol. Metab. 83 : 2223-2235. crossref(new window)

14.
Wang, X., X. Zhao, B. Yang, H. Dong, D. Liu, and L. Huang. 2011. A combination of ultrasonic-assisted extraction with RRLC-QQQ method for the determination of artemisin in the chinese herb Artemisia annua L. Phytochemical Analysis. 22(3) : 280-284. crossref(new window)

15.
Yoshiki, Y., J. H. Kim, and K. Okubo. 1994. Saponins conjugated with 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one from Phaseolus coccineus. Phytochemistry. 36(4) : 1009-1012. crossref(new window)

16.
Zhang, W., D. G. Popovich. 2008. Effect of soyasapogenol A and soyasapogenol B concentrated extracts on Hep-G2 cell proliferation and apoptosis. J. Agric. Food Chem. 56 : 2603-2608. crossref(new window)

17.
Zhang, W., S. P. Teng, D. G. Popovich. 2009. Generation of group B woyasaponins I and III by hydrolysis. J. Agric. Food Chem. 57 : 3620-3625. crossref(new window)