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HPLC/MS/MS Method for Determination of Soyasaponins in the Soybean Varieties
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  • Volume 56, Issue 3,  2011, pp.244-249
  • Publisher : The Korean Society of Crop Science
  • DOI : 10.7740/kjcs.2011.56.3.244
 Title & Authors
HPLC/MS/MS Method for Determination of Soyasaponins in the Soybean Varieties
Han, Sang-Jun;
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A sensitive and rapid high-performance liquid chromatographic-tandem mass spectrometric (HPLC/MS/MS) assay was developed for the determination of soyasaponins in soybean. Among soyasaponins, soyasaponin I was isolated and characterized from methanol extracts of soybean as analytical standards and the development of a new analytical procedure for quantification of its content in various cultivars. The structures of these compound was elucidated by , NMR experiments and by mass spectrometric analysis. Aqueous ethanol extracts of soybean samples were injected on an Agilent XDB-C18 column (, ) with a mobile phase consisting of 10 mM ammonium acetate-acetonitrile, a flow rate of 0.3 mL/min and a total run time of 8 min. Detection was performed by mass spectrometer bin the multiple reaction monitoring (MRM) mode with negative electrospray ionization (ESI) m/z at 941 615 for soyasaponin I. In the 9 soybean samples, contents of soyasaponin I ranged from 205 to 726 mg/kg, and correlated negatively with seed size.
 Cited by
미국으로부터 재도입된 한국 원산 콩 재래종의 Saponin 함량 변이,장은규;박향민;황태영;김선림;정규화;;최유미;이명철;이정란;김홍식;

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