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Antimicrobial Effect, Antioxidant and Tyrosinase Inhibitory Activity of the Extract from Different Parts of Phytolacca americana L.
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  • Volume 60, Issue 3,  2015, pp.366-373
  • Publisher : The Korean Society of Crop Science
  • DOI : 10.7740/kjcs.2015.60.3.366
 Title & Authors
Antimicrobial Effect, Antioxidant and Tyrosinase Inhibitory Activity of the Extract from Different Parts of Phytolacca americana L.
Boo, Hee-Ock; Park, Jeong-Hun; Woo, Sun-Hee; Park, Hyeon-Yong;
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This study was conducted to clarify the antimicrobial effect, antioxidant and tyrosinase inhibitory activities of the biological composition having the Phytolacca americana, and to enhance the natural materials utilization of foods and cosmetics. The antimicrobial activities of the different parts of P. americana were evaluated using the agar diffusion test. The antimicrobial activity of P. americana was relatively high in Malassezia furfur known as a skin fungi and Vibrio parahaemolyticus compared to Escherichia coli and Staphy-lococcus epidermidis. However, the antimicrobial activity in Vibrio parahaemolyticus did not show at all parts of P. americana. Both the DPPH radical scavenging activity and ABTS radical scavenging activity have been increased with the higher concentration of methanol extract. In particular, leaf extract of P. americana exhibited the highest activity both ABTS radical scavenging activity and DPPH radical scavenging activity. The nitrite scavenging activity was decreased when the pH was changed from pH 1.2 to pH 6.0. The highest nitrite scavenging activity was exhibited from the methanol extract of fruit, followed by root, stem, and leaf at pH 1.2. However, the nitrite scavenging activity at pH of 6.0 was not almost detected. All plant parts of P. americana showed tyrosinase inhibitory activity. The highest activity was found in the stem, and followed by root, leaf, and fruit in order. These tyrosinase inhibitory activity was progressively increased in a concentration-dependent manner. In this experiment on the methanol extracts of different organ from P. americana, we confirmed that the extract of P. americana showed potent tyrosinase inhibitory activity. Taken together, we conjectured that the P. americana had the potent biological activities, therefore this plant having various functional components could be a good material for development into source of natural food additives and cosmetics.
antimicrobial;DPPH;ABTS;nitrite;tyrosinase;Phytolacca americana;
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Comparative Analysis of Saponins from Different Phytolaccaceae Species and Their Antiproliferative Activities, Molecules, 2017, 22, 7, 1077  crossref(new windwow)
Boo, H. O., J. H. Shin, J. S. Shin, E. S. Choung, M. A. Bang, K. M. Choi, and W. S. Song. 2012. Assessment on Antioxidant Potential and Enzyme Activity of Some Economic Resource Plants. Korean J. Plant Res. 25(3) : 349-356. crossref(new window)

Brand-Williams, W., M. E. Cuvelier, and C. Berset. 1995. Use of a free radical method to evaluate antioxidant activity. Food Sci. Tech. 28 : 25-30.

Bylka, W. and I. Matlawska. 2001. Flavonoids and free phenolic acids from Phytolacca americana L. leaves. Acta Poloniae Pharmaceutica-Drug Research. 58(1) : 69-72.

Chan, L. W., E. L. C. Cheah, C. L. L. Saw, W. Weng, and P. W. Heng. 2008. Antimicrobial and antioxidant activities of Cortex Magnoliae Officinalis and some other medicinal plants commonly used in South-East Asia. Chinese Medicine. 3 : 15. crossref(new window)

Choi, D. B., K. A. Cho, M. S. Na, H. S. Choi, Y. O. Kim, D. H. Lim, S. J. Cho, and H. Cho. 2008. Effect of bamboo oil on antioxidative activity and nitrite scavenging activity. J. Ind. Eng. Chem. 14 : 765-770. crossref(new window)

Flurkey, A., J. Cooksey, A. Reddy, K. Spoonmore, A. Rescigno, J. Inlow, and W. H. Flurkey. 2008. Enzyme, protein, carbohydrate, and phenolic contaminants in commercial tyrosinase preparations; potential problems affecting tyrosinase activity and inhibition studies. J. Agric. Food Chem. 56 : 4760-4768. crossref(new window)

Foyer, C. H., P. Descourvieres, and K. J. Kunert. 1994. Protection against oxygen radicals: an important defence mechanism studied in transgenic plants. Plant Cell Env. 17 : 507-523. crossref(new window)

Goveas, S. W. and A. Abraham. 2013. Evaluation of antimicrobial and antioxidant activity of stem and leaf extracts of Coscinium fenestratum. Asian Journal of Pharmaceutical and Clinical Research. 6(3) : 218-221.

Hosseinimehr, S. J., F. Pourmorad, and N. Shahabimajd. 2007. In vitro antioxidant activity of Polygonium hyrcanicum, Centaurea depressa, Sambucus ebulus, Mentha spicata and Phytolacca americana. Pakistan Journal of Biological Sciences. 10(4) : 637-640. crossref(new window)

Hong, T. G., Y. R. Lee, M. H. Yim, and C. N. Hyun. 2004. Physiological Functionality and Nitrite Scavenging Ability of Fermentation Extracts from Pine Needles. Korean J Food Preserv. 11 : 94-99.

Iwu, M. W., A. R. Duncan, and C. O. Okunji. 1999. New antimicrobials of plant origin. In: Janick J. ed. Perspectives on New Crops and New Uses. Alexandria, VA: ASHS Press. pp. 457-462.

Kato, H., I. E. Lee, N. V. Chuyen, S. B. Kim and F. Hayase. 1987. Inhibition of nitrosamine formation by nondialyzable melanoidins. Agric. Biol. Chem. 51 : 1333-1338. crossref(new window)

Kato, F. T. and T. T. Puck. 1971. Mutagenesis by carcinogenic nitroso compounds. J Cell Physiol. 78 : 139-144. crossref(new window)

Khanom, F., H. Kayahara and K. Tadasa. 2000. Tyrosinase inhibitory activity of Bangladeshi indigenous medicinal plants. Biosci. Biotechnol. Biochem. 64(9) : 1967-1969. crossref(new window)

Kim, Y. W., Y. S. Yoon, M. R. Kim, S. H. Park, and J. C. Choi. 2008. Two Cases of Phytolacca Americana Intoxication with Confusion and Abdominal Cramping. Journal of The Korean Society of Chemical Toxicology. 6(2) : 146-148.

Lee, B. W., J. H. Lee, S. W. Gal, Y. H. Moon, and K. H. Park. 2006. Selective ABTS radical scavenging activity of prenylated flavonoids from Cudrania tricuspidata. Biosci. Biotechnol. Biochem. 70(2) : 427-432. crossref(new window)

Lee, S. C., S. Y. Kim, S. M. Jeong, and J. H. Park. 2006. Effect of far-infrared irradiation on catechins and nitrite scavenging activity of green tea. J. Agric. Food Chem. 54 : 399-403. crossref(new window)

Marmol, V., F. Solano, A. Sels, G. Huez, A. Libert, F. Lejeune, and G. Ghanem. 1993. Glutathione depletion increases tyrosinase activity in human melanoma cells. J. Invest. Dermatol. 101 : 871-874. crossref(new window)

Modaressi, M., R. Shahsavari, F. Ahmadi, M. Rahimi-Nasrabadi, R. Abiri, A. Mikaeli, and H. Batoli. 2013. The evaluation of antibacterial, antifungal and antioxidant activity of methanolic extract of Mindium Laevigatum (Vent.) Rech. F., from central part of Iran. Jundishapur J. Nat. Pharm. Prod. 8(1) : 34-40. crossref(new window)

Nevil, A. C. 1975. "Zoophysiology and Ecology". Springer-Verlag. New York. Vols. 4 and 5.

Prakash, A., F. Rigelhof, and E. Miller. 2001. Antioxidant activity. Analytical progress (Medallion laboratories). 1-4.

Parekh, J. and S. V. Chanda. 2007. In vitro antimicrobial activity and phytochemical analysis of some Indian medicinal plants. Turk. J. Biol. 31 : 53-58.

Re, R., N. Pellegrini, A. Proteggente, A. Pannala, M. Yang, and C. R. Evans. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine. 26(9-10) : 1231-1237. crossref(new window)

Shao, F., Z. Hu, Y. M. Xiong, Q. Z. Huang, C. G. Wang, R. H. Zhu, and D. C. Wang. 1999. A new antifungal peptide from the seeds of Phytolacca americana : characterization, amino acid sequence and cDNA cloning. Biochimica et Biophysica Acta. 1430 : 262-268. crossref(new window)

Stainer, R. Y., J. L. Ingraham, and M. L. Wheelis. 1986. General Microbiology, 5th ed. London. The MacMillan Press Ltd.

Tiwari, A. K. 2001. Imbalance in antioxidant defence and human diseases: Multiple approach of natural antioxidants therapy. Curr. Sci. 81 : 1179-1187.

Saija, A. D., A. Trombetta, R. L. Tomaino, P. Cascio, N. Princi, F. B. Uccella, and F. Castelli. 1998. 'In vitro' evaluation of the antioxidant activity and biomembrane interaction of the plant phenols oleu-ropein and hydroxytyrosol. Int J Pharmacol 166 : 123-133. crossref(new window)

Sanchez, C. S., A. M. T. Gozalez, M. C. Garcia-Parrilla, J. J. Q. Granados, H. L. G. Serrana, and M. C. L. Martinez. 2007. Different radical scavenging tests in virgin olive oil and their relation to the total phenol content. Anal. Chemical Acta. 593 : 103-107. crossref(new window)

Shin, J. H., J. Y. Lee, J. C. Ju, S. J. Lee, H. S. Cho, and N. J. Sung. 2005. Chemical Properties and Nitrite Scavenging Ability of Citron (Citrus junos). J. Korean Soc. Food Sci. Nutr. 34 : 496-502. crossref(new window)

Wang, L., L. Bai, T. Nagasawa, T. Hasegawa, X. Yang, J. Sakai, Y. Bai, T. Kataoka, S. Oka, K. Hirose, A. Tomida, T. Tsuruo, and M. Ando. 2008. Bioactive triterpene saponins from the roots of Phytolacca americana. Journal of National Products. 71(1) : 35-40. crossref(new window)