Advanced SearchSearch Tips
Proteomic Responses of Diploid and Tetraploid Roots in Platycodon grandiflorum
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Volume 60, Issue 3,  2015, pp.394-400
  • Publisher : The Korean Society of Crop Science
  • DOI : 10.7740/kjcs.2015.60.3.394
 Title & Authors
Proteomic Responses of Diploid and Tetraploid Roots in Platycodon grandiflorum
Kim, Hye-Rim; Kwon, Soo-Jeong; Roy, Swapan Kumar; Cho, Seong-Woo; Kim, Hag-Hyun; Moon, Young-Ja; Boo, Hee-Ock; Woo, Sun-Hee;
  PDF(new window)
The roots of Platycodon grandiflorum species either dried or fresh, are used as an ingredient in salads and traditional cuisine in Korea. To interpret the root proteins, a systematical and targeting analysis were carried out from diploid and tetraploid roots. Two dimensional gels stained with CBB, a total of 39 differential expressed proteins were identified from the diploid root under in vivo condition using image analysis by Progenesis Same Spot software. Out of total differential expressed spots, 39 differential expressed protein spots (-fold) were analyzed using LTQ-FTICR mass spectrometry. Except two proteins, the rest of the identified proteins were confirmed as down-regulated such as Isocitrate dehydrogenase, Proteasome subunit alpha type-2-B. However, the most of the identified proteins from the explants were mainly associated with the oxidoreductase activity, nucleic acid binding, transferase activity and catalytic activity. The exclusive protein profile may provide insight clues for better understanding the characteristics of proteins and metabolic activity in various explants of the economically important medicinal plant Platycodon grandiflorum.
diploid root;tetraploid root;in vivo;glycolysis pathway;Platycodon grandiflorum;
 Cited by
Fritsche, O. and W. Junge. 1996. Chloroplast ATP synthase: the clutch between proton flow and ATP synthesis is at the interface of subunit and $CF^1$. Biochimica Biophysica Acta 1274 : 94-100. crossref(new window)

Goward, C. R. and D. J. Nicholls. 1994. Malate dehydrogenase: A model for structure, evolution, and catalysis. Protein Sci. 3 : 1883-1888. crossref(new window)

Grossman, A. R., D. Bhaya, K. EApt, and D. M. Kehoe. 1995. Light-Harvesting Complexes in Oxygenic Photosynthesis: Diversity, Control, and Evolution. Annual Review of Genetics 29 : 231-288. crossref(new window)

Kamal, A. H., K. Cho, S. Komatsu, N. Uozumi, J. S. Choi, and S. H. Woo. 2012. Towards an understanding of wheat chloroplasts: a methodical investigation of thylakoid proteome. Mol Biol Rep 39 : 5069-83. crossref(new window)

Kenmochi, N., L. K. Ashworth, G. Lennon, S. Higa, and T. Tanaka. 1998. High-Resolution Mapping of Ribosomal Protein Genes to Human Chromosome 19. DNA Res. 5 : 229-233. crossref(new window)

Kim, K. H., A. H. M Kamal, K. H. Shin, J. S. Choi, H. Y. Heo, and S. H. Woo. 2010. Large scale proteomic investigation in wild relatives (A, B and D genomes) of wheat. Acta Biochimica et Biophysica Sinica 42 : 709-716. crossref(new window)

Komatsu, S., Y. Nanjo, and M. Nishimura. 2013. Proteomic analysis of the flooding tolerance mechanism in mutant soybean. Journal of Proteomics 79 : 231-250. crossref(new window)

Lee, C. P. 2009. Dynamics of the plant mitochondrial proteome : Towards the understanding of metabolic networks, Ph.D. Thesis, The University of Western Australia.

Link, A. J., J. Eng, D. M. Schieltz, E. Carmack, G. J. Mize, D. R. Morris, B. M. Garvik, J. R 3rd Yates. 1999. Direct analysis of protein complexes using mass spectrometry. Nat. Biotechnol. 17(7) : 676-682. crossref(new window)

Meunier, B., E. Dumas, I. Piec, D. Bechet, M. Hebraud, and J. F. Hocquette. 2007. Assessment of hierarchical clustering methodologies for proteomic data mining. J. Proteome Res. 6(1) : 358-366. crossref(new window)

Millar, A. H., J. L. Heazlewood, B. K. Kristensen, H. P. Braun, and I. M. Moller. 2005. The plant mitochondrial proteome, TRENDS in Plant Science, 10 (1) : 36-43.

Olsen, J. V. and M. Mann. 2006. Improved peptide identification in proteomics by two consecutive stages of mass spectrometric fragmentation, Proceedings of the National Academy of Sciences, 101(37) : 13417-13422.

Schweizer, P., W. Hunziker, and E. Mosinger. 1989. cDNA cloning, in vitro transcription and partial sequence analysis of mRNAs from winter wheat (Triticum aestivum L.) with induced resistance to Erysiphe graminins f. sp. tvitici. Plant Mol. Biol. 12 : 643-654. crossref(new window)

Washburn, M. P., D. Wolters, J. R. 3rd Yates. 2001. Large-scale analysis of the yeast proteome by multidimensional protein identification technology. Nat. Biotechnol. 19(3) : 242-247. crossref(new window)

Wolters, D. A., M. P. Washburn, J. R. Yates JR., 3rd 2001. An automated multidimensional protein identification technology for shotgun proteomics. Anal Chem. 73 : 5683-5690. crossref(new window)

Woo, S. H., H. S. Kim, B. H. Song, C. W. Lee, Y. M. Park, S. K. Jong, and Y. G. Cho. 2003. Rice proteomics: a functional analysis of the rice genome and applications. Korean J. Plant Biotechnol. 30(3) : 261-191.