Advanced SearchSearch Tips
Further observations on the genetics and morphometrics of Coolia santacroce (Dinophyceae)
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : ALGAE
  • Volume 30, Issue 4,  2015, pp.275-280
  • Publisher : The Korean Society of Phycology
  • DOI : 10.4490/algae.2015.30.4.275
 Title & Authors
Further observations on the genetics and morphometrics of Coolia santacroce (Dinophyceae)
Karafas, Sarah J.; Tomas, Carmelo R.;
  PDF(new window)
Coolia santacroce is a newly described epibenthic dinoflagellate species collected from the U.S. Virgin Islands. The original description indicates this species is unique from others in the Coolia monotis complex due to the relative size of the apical pore complex, broad range of pore sizes, and ribosomal DNA. The original description was done based on the isolation and cultivation of one isolate of the organism. In this study, we report three more isolates of Coolia santacroce collected from the Bahamas. Morphological observations were made using scanning electron microscopy that do not correspond to those from the original description, indicating the variability of the morphological features. However, analysis of the D1 / D2 regions of the large subunit rDNA places the three strains in a strongly supported monophyletic clade with the type specimen.
 Cited by
Molecular phylogeny and toxicity of harmful benthic dinoflagellates Coolia (Ostreopsidaceae, Dinophyceae) in a sub-tropical marine ecosystem: The first record from Hong Kong, Marine Pollution Bulletin, 2017  crossref(new windwow)
David, H., Laza-Martínez, A., Miguel, I. & Orive, E. 2014. Broad distribution of Coolia monotis and restricted distribution of Coolia cf. canariensis (Dinophyceae) on the Atlantic coast of the Iberian Peninsula. Phycologia 53:342-352. crossref(new window)

Dolapsakis, N. P., Kilpatrick, M. W., Economou-Amilli, A. & Tafas, T. 2006. Morphology and rDNA phylogeny of a Mediterranean Coolia monotis (Dinophyceae) strain from Greece. Sci. Mar. 70:67-76.

Faust, M. A. 1995. Observation of sand-dwelling toxic dinoflagellates (Dinophyceae) from widely differing sites, in cluding two new species. J. Phycol. 31:996-1003. crossref(new window)

Fraga, S., Penna, A., Bianconi, I., Paz, B. & Zapata, M. 2008. Coolia canariensis sp. nov. (Dinophyceae), a new nontoxic epiphytic benthic dinoflagellate from the Canary Islands. J. Phycol. 44:1060-1070. crossref(new window)

Jeong, H. J., Yih, W., Kang, N. S., Lee, S. Y., Yoon, E. Y., Yoo, Y. D., Kim, H. S. & Kim, J. H. 2012. First report of the epiphytic benthic dinoflagellates Coolia canariensis and Coolia malayensis in the waters off Jeju Island, Korea: morphology and rDNA sequences. J. Eukaryot. Microbiol. 59:114- 133. crossref(new window)

Karafas, S., York, R. & Tomas, C. 2015. Morphological and genetic analysis of the Coolia monotis species complex with the introduction of two new species, Coolia santacroce sp. nov. and Coolia palmyrensis sp. nov. (Dinophyceae). Harmful Algae 46:18-33. crossref(new window)

Laza-Martínez, A., Orive, E. & Miguel, I. 2011. Morphological and genetic characterization of benthic dinoflagellates of the genera Coolia, Ostreopsis, and Prorocentrum from the south-eastern Bay of Biscay. Eur. J. Phycol. 46:45-65.

Leaw, C. -P., Lim, P. -T., Cheng, K. -W., Ng, B. -K. & Usup, G. 2010. Morphology and molecular characterization of a new species of thecate benthic dinoflagellate, Coolia malayensis sp. nov (Dinophyceae). J. Phycol. 46:162-171. crossref(new window)

Meunier, A. 1919. Microplancton de la Mer Flamande. 3. Les Péridiniens. Mem. Mus. R. Hist. Nat. Bruxelles 8:3-116.

Mohammad-Noor, N., Moestrup, Ø., Lundholm, N., Fraga, S., Adam, A., Holmes, M. J. & Saleh, E. 2013. Autecology and phylogeny of Coolia tropicalis and Coolia malayensis (Dinophyceae), with emphasis on taxonomy of C. tropicalis based on light microscopy, scanning electron microscopy and LSU rDNA. J. Phycol. 49:536-545. crossref(new window)

Momigliano, P., Sparrow, L., Blair, D. & Heimann, K. 2013. The diversity of Coolia spp. (Dinophyceae: Ostreopsidaceae) in the Central Great Barrier Reef Region. PLoS ONE 8:e79278. crossref(new window)

Penna, A., Vila, M., Fraga, S., Giacobbe, M. G., Andreoni, F., Riobó, P. & Vernesi, C. 2005. Characterization of Ostreopsis and Coolia (Dinophyceae) isolates in the western Mediterranean Sea based on morphology, toxicity, and internal transcribed spacer 5.8s rDNA sequences. J. Phycol. 41:212-225. crossref(new window)

Rhodes, L., Smith, K., Gimenez Papiol, G., Adamson, J., Harwood, T. & Munday, R. 2014. Epiphytic dinoflagellates in sub-tropical New Zealand, in particular the genus Coolia Meunier. Harmful Algae 34:36-41. crossref(new window)

Richlen, M. L. & Barber, P. H. 2005. A technique for the rapid extraction of microalgal DNA from single live and preserved cells. Mol. Ecol. Notes 5:688-691. crossref(new window)

Scholin, C. A., Herzog, M., Sogin, M. & Anderson, D. M. 1994. Identification of group- and strain-specific genetic markers for globally distributed Alexandrium (Dinophyceae). II. Sequence analysis of a fragment of the LSU rRNA gene. J. Phycol. 30:999-1011. crossref(new window)

Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. & Kumar, S. 2011. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol. 28:2731-2739. crossref(new window)

Ten-Hage, L., Turquet, J., Quod, J. P. & Couté, A. 2000. Coolia areolata sp. nov (Dinophyceae), a new sand-dwelling dinoflagellate from the southwestern Indian Ocean. Phycologia 39:377-383. crossref(new window)