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Transcriptome analysis of the short-term photosynthetic sea slug Placida dendritica
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  • Journal title : ALGAE
  • Volume 30, Issue 4,  2015, pp.303-312
  • Publisher : The Korean Society of Phycology
  • DOI : 10.4490/algae.2015.30.4.303
 Title & Authors
Transcriptome analysis of the short-term photosynthetic sea slug Placida dendritica
Han, Ji Hee; Klochkova, Tatyana A.; Han, Jong Won; Shim, Junbo; Kim, Gwang Hoon;
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The intimate physical interaction between food algae and sacoglossan sea slug is a pertinent system to test the theory that “you are what you eat.” Some sacoglossan mollusks ingest and maintain chloroplasts that they acquire from the algae for photosynthesis. The basis of photosynthesis maintenance in these sea slugs was often explained by extensive horizontal gene transfer (HGT) from the food algae to the animal nucleus. Two large-scale expressed sequence tags databases of the green alga Bryopsis plumosa and sea slug Placida dendritica were established using 454 pyrosequencing. Comparison of the transcriptomes showed no possible case of putative HGT, except an actin gene from P. dendritica, designated as PdActin04, which showed 98.9% identity in DNA sequence with the complementary gene from B. plumosa, BpActin03. Highly conserved homologues of this actin gene were found from related green algae, but not in other photosynthetic sea slugs. Phylogenetic analysis showed incongruence between the gene and known organismal phylogenies of the two species. Our data suggest that HGT is not the primary reason underlying the maintenance of short-term kleptoplastidy in Placida dendritica.
actin;Bryopsis plumosa;horizontal gene transfer;kleptoplastidy;Placida dendritica;transcriptome;
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Bhattacharya, D., Pelletreau, K. N., Price, D. C., Sarver, K. E. & Rumpho, M. E. 2013. Genome analysis of Elysia chlorotica egg DNA provides no evidence for horizontal gene transfer into the germ line of this kleptoplastic mollusc. Mol. Biol. Evol. 30:1843-1852. crossref(new window)

Evertsen, J. & Johnsen, G. 2009. In vivo and in vitro differences in chloroplast functionality in the two north Atlantic sacoglossans (Gastropoda, Opisthobranchia) Placida dendritica and Elysia viridis. Mar. Biol. 156:847-859. crossref(new window)

Keeling, P. J. & Palmer, J. D. 2008. Horizontal gene transfer in eukaryotic evolution. Nat. Rev. Genet. 9:605-618. crossref(new window)

Kleffmann, T., Russenberger, D., von Zychlinski, A., Christopher, W., Sjölander, K., Gruissem, W. & Baginsky, S. 2004. The Arabidopsis thaliana chloroplast proteome reveals pathway abundance and novel protein functions. Curr. Biol. 14:354-362. crossref(new window)

Klochkova, T. A., Han, J. W., Chah, K. -H., Kim, R. W., Kim, J. -H., Kim, K. Y. & Kim, G. H. 2013. Morphology, molecular phylogeny and photosynthetic activity of the sacoglossan mollusc, Elysia nigrocapitata, from Korea. Mar. Biol. 160:155-168. crossref(new window)

Klochkova, T. A., Han, J. W., Kim, J. -H., Kim, K. Y. & Kim, G. H. 2010. Feeding specificity and photosynthetic activity of Korean sacoglossan mollusks. Algae 25:217-227. crossref(new window)

Rumpho, M. E., Pelletreau, K. N., Moustafa, A. & Bhattacharya, D. 2011. The making of a photosynthetic animal. J. Exp. Bot. 214:303-311.

Wägele, H., Deusch, O., Händeler, K., Martin, R., Schmitt, V., Christa, G., Pinzger, B., Gould, S. B., Dagan, T., Klussmann-Kolb, A. & Martin, W. 2011. Transcriptomic evidence that longevity of acquired plastids in the photosynthetic slugs Elysia timida and Plakobranchus ocellatus does not entail lateral transfer of algal nuclear genes. Mol. Biol. Evol. 28:699-706. crossref(new window)