JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Evaluation of phlorofucofuroeckol-A isolated from Ecklonia cava (Phaeophyta) on anti-lipid peroxidation in vitro and in vivo
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : ALGAE
  • Volume 30, Issue 4,  2015, pp.313-323
  • Publisher : The Korean Society of Phycology
  • DOI : 10.4490/algae.2015.30.4.313
 Title & Authors
Evaluation of phlorofucofuroeckol-A isolated from Ecklonia cava (Phaeophyta) on anti-lipid peroxidation in vitro and in vivo
Lee, Ji-Hyeok; Ko, Ju-Young; Oh, Jae-Young; Kim, Eun-A; Kim, Chul-Young; Jeon, You-Jin;
  PDF(new window)
 Abstract
Lipid peroxidation means the oxidative degradation of lipids. The process from the cell membrane lipids in an organism is generated by free radicals, and result in cell damage. Phlorotannins, well-known marine brown algal polyphenols, have been utilized in functional food supplements as well as in medicine supplements to serve a variety of purposes. In this study, we assessed the potential anti-lipid peroxidation activity of phlorofucofuroeckol-A (PFF-A), one of the phlorotannins, isolated from Ecklonia cava by centrifugal partition chromatography in 2,2-azobis (2-amidinopropane) dihydrochloride (AAPH)-stimulated Vero cells and zebrafish system. PFF-A showed the strongest scavenging activity against alkyl radicals of all other reactive oxygen species (ROS) and exhibited a strong protective effect against ROS and a significantly strong inhibited of malondialdehyde in AAPH-stimulated Vero cells. The apoptotic bodies and pro-apoptotic proteins Bax and caspase-3, which were induced by AAPH, were strongly inhibited by PFF-A in a dose-dependent manner and expression of Bcl-xL, an anti-apoptotic protein, was induced. In the AAPH-stimulated zebrafish model, additionally PFF-A significantly inhibited ROS and cell death, as well as exhibited a strong protective effect against lipid peroxidation. Therefore, these results suggest that PFF-A has excellent protective effects against ROS and lipid peroxidation induced by AAPH in both an in vitro Vero cell model and an in vivo zebrafish model.
 Keywords
AAPH;Ecklonia cava;lipid peroxidation;phlorofucofuroeckol-A;Vero cell model;zebrafish model;
 Language
English
 Cited by
1.
Popular edible seaweed, Gelidium amansii prevents against diet-induced obesity, Food and Chemical Toxicology, 2016, 90, 181  crossref(new windwow)
2.
A marine algal polyphenol, dieckol, attenuates blood glucose levels by Akt pathway in alloxan induced hyperglycemia zebrafish model, RSC Adv., 2016, 6, 82, 78570  crossref(new windwow)
 References
1.
Artan, M., Li, Y., Karadeniz, F., Lee, S. -H., Kim, M. -M. & Kim, S. -K. 2008. Anti-HIV-1 activity of phloroglucinol derivative, 6,6′-bieckol, from Ecklonia cava. Bioorg. Med. Chem. 16:7921-7926. crossref(new window)

2.
Bast, A., Haenen, G. R. M. M. & Doelman, C. J. A. 1991. Oxidants and antioxidants: state of the art. Am. J. Med. 91(Suppl. 3):S2-S13.

3.
Cory, S. & Adams, J. M. 1998. Matters of life and death: programmed cell death at Cold Spring Harbor. Biochim. Biophys. Acta 1377:R25-R44.

4.
Eom, S. -H., Kim, Y. -M. & Kim, S. -K. 2012. Antimicrobial effect of phlorotannins from marine brown algae. Food Chem. Toxicol. 50:3251-3255. crossref(new window)

5.
Er, E., Oliver, L., Cartron, P. -F., Juin, P., Manon, S. & Vallette, F. M. 2006. Mitochondria as the target of the pro-apoptotic protein Bax. Biochim. Biophys. Acta 1757:1301-1311. crossref(new window)

6.
Esterbauer, H., Gebicki, J., Puhl, H. & Jürgens, G. 1992. The role of lipid peroxidation and antioxidants in oxidative modification of LDL. Free Radic. Biol. Med. 13:341-390. crossref(new window)

7.
Faure, P., Oziol, L., Artur, Y. & Chomard, P. 2004. Thyroid hormone (T3) and its acetic derivative (TA3) protect low-density lipoproteins from oxidation by different mechanisms. Biochimie 86:411-418. crossref(new window)

8.
Halliwell, B., Hu, M. L., Louie, S., Duvall, T. R., Tarkington, B. K., Motchnik, P. & Cross, C. E. 1992. Interaction of nitrogen dioxide with human plasma: antioxidant depletion and oxidative damage. FEBS Lett. 313:62-66. crossref(new window)

9.
Heath, R. L. & Packer, L. 1968. Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys. 125:189-198. crossref(new window)

10.
Hu, C. & Kitts, D. D. 2005. Dandelion (Taraxacum officinale) flower extract suppresses both reactive oxygen species and nitric oxide and prevents lipid oxidation in vitro. Phytomedicine 12:588-597. crossref(new window)

11.
Jain, K., Kataria, S. & Guruprasad, K. N. 2003. Changes in antioxidant defenses of cucumber cotyledons in response to UV-B and to the free radical generating compound AAPH. Plant Sci. 165:551-557. crossref(new window)

12.
Kang, K. S., Kim, H. Y., Yoo, H. H., Piao, X. L., Ham, J., Yang, H. O. & Park, J. H. 2012a. Protective effect of ginseng sapogenins against 2,2′-azobis (1-aminopropane) dihydrochloride (AAPH)-induced LLC-PK1 cell damage. Bioorg. Med. Chem. Lett. 22:634-637. crossref(new window)

13.
Kang, M. -C., Cha, S. H., Wijesinghe, W. A. J. P., Kang, S. -M., Lee, S. -H., Kim, E. -A., Song, C. B. & Jeon, Y. -J. 2013. Protective effect of marine algae phlorotannins against AAPH-induced oxidative stress in zebrafish embryo. Food Chem. 138:950-955. crossref(new window)

14.
Kang, S. -M., Heo, S. -J., Kim, K. -N., Lee, S. -H. & Jeon, Y. -J. 2012b. Isolation and identification of new compound, 2,7″-phloroglucinol-6,6′-bieckol from brown algae, Ecklonia cava and its antioxidant effect. J. Funct. Foods 4:158-166. crossref(new window)

15.
Kim, A. -R., Lee, M. -S., Shin, T. -S., Hua, H., Jang, B. -C., Choi, J. -S., Byun, D. -S., Utsuki, T., Ingram, D. & Kim, H. -R. 2011. Phlorofucofuroeckol A inhibits the LPS-stimulated iNOS and COX-2 expressions in macrophages via inhibition of NF-κB, Akt, and p38 MAPK. Toxicol. In Vitro 25:1789-1795.

16.
Kim, H. K. 2014. Role of ERK/MAPK signalling pathway in anti-inflammatory effects of Ecklonia cava in activated human mast cell line-1 cells. Asian Pac. J. Trop. Med. 7:703-708. crossref(new window)

17.
Ko, J. -Y., Kim, E. -A., Lee, J. -H., Kang, M. -C., Lee, J. -S., Kim, J. -S., Jung, W. -K. & Jeon, Y. -J. 2014. Protective effect of aquacultured flounder fish-derived peptide against oxidative stress in zebrafish. Fish Shellfish Immunol. 36:320-323. crossref(new window)

18.
Ko, S. -C., Lee, M., Lee, J. -H., Lee, S. -H., Lim, Y. & Jeon, Y. -J. 2013. Dieckol, a phlorotannin isolated from a brown seaweed, Ecklonia cava, inhibits adipogenesis through AMP-activated protein kinase (AMPK) activation in 3T3-L1 preadipocytes. Environ. Toxicol. Pharmacol. 36:1253-1260. crossref(new window)

19.
Le, Q. -T., Li, Y., Qian, Z. -J., Kim, M. -M. & Kim, S. -K. 2009. Inhibitory effects of polyphenols isolated from marine alga Ecklonia cava on histamine release. Proc. Biochem. 44:168-176. crossref(new window)

20.
Lee, J. -H., Ko, J. -Y., Oh, J. -Y., Kim, C. -Y., Lee, H. -J., Kim, J. & Jeon, Y. -J. 2014. Preparative isolation and purification of phlorotannins from Ecklonia cava using centrifugal partition chromatography by one-step. Food Chem. 158:433-437. crossref(new window)

21.
Lee, J. -H., Zhang, C., Ko, J. -Y., Lee, J. -S. & Jeon, Y. -J. 2015. Evaluation on anticancer effect against HL-60 cells and toxicity in vitro and in vivo of the phenethyl acetate isolated from a marine bacterium, Streptomyces griseus. Fish. Aquat. Sci. 18:35-44.

22.
Lee, S. -H., Kang, M. -C., Moon, S. -H., Jeon, B. -T. & Jeon, Y. -J. 2013. Potential use of ultrasound in antioxidant extraction from Ecklonia cava. Algae 28:371-378. crossref(new window)

23.
Li, Y., Qian, Z. -J., Ryu, B. M., Lee, S. -H., Kim, M. -M. & Kim, S. -K. 2009. Chemical components and its antioxidant properties in vitro: an edible marine brown alga, Ecklonia cava. Bioorg. Med. Chem. 17:1963-1973. crossref(new window)

24.
Ling, J., Wei, B., Lv, G., Ji, H. & Li, S. 2012. Anti-hyperlipidaemic and antioxidant effects of turmeric oil in hyperlipidaemic rats. Food Chem. 130:229-235. crossref(new window)

25.
Mayo, J. C., Tan, D. X., Sainz, R. M., Natarajan, M., Lopez-Burillo, S. & Reiter, R. J. 2003. Protection against oxidative protein damage induced by metal-catalyzed reaction or alkylperoxyl radicals: comparative effects of melatonin and other antioxidants. Biochim. Biophys. Acta 1620:139-150. crossref(new window)

26.
Miki, M., Tamai, H., Mino, M., Yamamoto, Y. & Niki, E. 1987. Free-radical chain oxidation of rat red blood cells by molecular oxygen and its inhibition by α-tocopherol. Arch. Biochem. Biophys. 258:373-380. crossref(new window)

27.
Niki, E. 1990. Free radical initiators as source of water-or lipid-soluble peroxyl radicals. Methods Enzymol. 186:100-108. crossref(new window)

28.
Qin, F., Rounds, N. K., Mao, W., Kawai, K. & Liang, C. S. 2001. Antioxidant vitamins prevent cardiomyocyte apoptosis produced by norepinephrine infusion in ferrets. Cardiovasc. Res. 51:736-748. crossref(new window)

29.
Sakr, S. A. & Abel-Samie, H. A. 2008. Apoptosis related protein Bax in liver of metalazxyl fungicide-treated mice: the effect of antox. Ozean J. Appl. Sci. 1:17-27.

30.
Shim, S. -Y., Choi, J. -S. & Byun, D. -S. 2009. Inhibitory effects of phloroglucinol derivatives isolated from Ecklonia stolonifera on FcεRI expression. Bioorg. Med. Chem. 17:4734-4739. crossref(new window)

31.
Yokozawa, T., Liu, Z. W. & Chen, C. P. 2000. Protective effects of Glycyrrhizae radix extract and its compounds in a renal hypoxia (ischemia)-reoxygenation (reperfusion) model. Phytomedicine 6:439-445. crossref(new window)