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Karyotype Analysis and Physical Mapping of rDNAs Using Bicolor-FISH in Tiarella polyphylla D. Don
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 Title & Authors
Karyotype Analysis and Physical Mapping of rDNAs Using Bicolor-FISH in Tiarella polyphylla D. Don
Kim, Soo-Young; Lee, Joong-Ku;
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 Abstract
Tiarella polyphylla D. Don(Saxifragaceae) is a perennial herb and distributed in China, Japan, Taiwan and Korea. Especially, it only grows in Ulleung island of Korea. It has been using for asthma, bruise and audition troubles with main components of some Triterpenoids and seven oleanolic Saponins. There is only known its chromosomal number rarely and cytogenetic study was not done. From this study, karyotype analysis and chromosomal localization of 5S and 45S rDNAs using bicolor-FISH(fluorescence in situ hybridization) were carried out. The somatic metaphase chromosome number was 2n=2x=14 and the size of chromosomes ranged . The chromosome complement consisted of four pairs of submetacentrics(chromosomes 1, 2, 3 and 6), two pairs of subtelocentrics(chromosomes 5 and 7) and one pair of telocentrics(chromosome 4). We also observed NOR(nucleolus organizer region) on the chromosome 4. In bicolor-FISH, one pair of 55 and 45S rDNA sites was detected on the centromeric region of chromosome 3 and short arm of chromosome 4, respectively. Bicolor FISH was very useful tool for the localization and identification of rDNAs on the chromosomes in Tiarella polyphylla.
 Keywords
Tiarella polyphylla;Karyotype analysis;Bicolor-FISH;5S rDNA;45S rDNA;
 Language
Korean
 Cited by
1.
정선황기의 세포유전학적 연구,한상은;김현희;허권;

식물분류학회지, 2013. vol.43. 2, pp.139-145 crossref(new window)
 References
1.
Benabdelmouna, A. and A.M. Darmency. 1997. Distribution and chromosomal organization of 18S-5.8S-25S and 5S rDNA in Petunia species. Agronomie (Plant Genet Breed) 17: 349-360

2.
Castilho, A. and J.S. Heslop-Harrison. 1995. Physical mapping of 5S and 18S-25S rDNA and repetitive DNA sequences in Aegilops umbellulata. Genome 38: 91-96

3.
D'Hont, A. Ison, K. Alix, C. Roux and J.G. Glaszmann. 1998. Determination of basic chromosome numbers in the genus Saccharum by physical mapping of ribosomal RNA genes. Genome 41: 221-225 crossref(new window)

4.
Fukui, K., N. Ohmido and G.S. Khush. 1994. Variability in rDNA loci in the genus Oryza detected through fluorescence in situ hybridization. Theor. Appl. Genet. 87: 893-899

5.
Funamoto, T. and T. Nakamura. 1991. Karyomorphological study on Japanese Tiarella polyphylla (Saxifragaceae). Chromosome Information Service 51: 14-15

6.
Hasterok, R., G. Jenkins, T. Langdon, R.N. Jones and J. Maluszynska. 2001. Ribosomal DNA is an effective marker of Brassica chromosomes. Theor. Appl. Genet. 103: 486-490 crossref(new window)

7.
Han, Y. H., L.J. Li, Y.C. Song, Z.Y. Li, Z.Y. Xiong and D.Y. Li. 2002. Physical mapping of the 5S and 45S rDNA in teosintes. Hereditas 137: 16-19 crossref(new window)

8.
Kim, S.Y., H.W. Choi and J.W. Bang. 2004.Physical mapping of rDNAs using McFISH in Anemarrhena asphodeloides Bunge. Korean J. Med. Crop Sci. 12: 515-518 (in Korean)

9.
Kim, S.Y., H.W. Choi, D.H. Koo, C.S. Kim and J.W. Bang. 2005. Karyotype analysis and physical mapping of rDNAs using McFISH in Jeffersonia dubia Benth. Korean J. Med. Crop Sci. 13: 48-51 (in Korean)

10.
Kim, S.Y., H.W. Choi, C.S. Kim, J.S. Sung, J.K. Lee and J.W. Bang. 2006a. Cytogenetic analysis of Astragalus species. Korean J. Med. Crop Sci. 14: 250-254 (in Korean)

11.
Kim, S.Y., J.W. Bang and J.K. Lee. 2006b. Cytogenetic analysis using mitosis, meiosis chromosomes and bicolor fluorescence in situ hybridization of Bupleurum latissimum Nakai. Korean J. Med. Crop Sci. 14: 354-359 (in Korean)

12.
Kim, S.Y., H.W. Choi, D.H. Koo, W.K. Lee, J.K. Lee and J.W. Bang. 2006c. Characterization of eight Rumex species by FISH (fluorescence in situ hybridization) and 5S rDNA spacer sequences. Korean J. Genetics 28: 243-251 (in Korean)

13.
Koo, D.H., N.S. Seong, J.S. Seong, K.H. Bang and J.W. Bang. 2003. Karyotype analysis and physical mapping of rDNAs in Bupleurum longeradiatum. Korean J. Crop Sci. 11: 402-407 (in Korean)

14.
Kurosawa, S. 1966. Cytological studies on some Eastern Himalayan plants. In The flora of Eastern Himalaya (Complied by Hitoshi Hava, Univ. of Tokyo Press. Japan). pp. 658-670

15.
Lee, W.K., H.W. Choi, D.H. Koo, S.Y. Kim and J.W. Bang. 2005. Molecular cytogenetics of five Pulsatilla species to the 5S, 45S rDNA genes by fluorescence in situ hybridization. Korean J. Genetics 27: 179-185 (in Korean)

16.
Lee, T.B. 1980. Illustrated Flora of Korea. Hyangmoonsa Co., Ltd (in Korean)

17.
Leitch, I.J. and J.S. Heslop-Harrison. 1992. Physical mapping of the 18S-5.8S-26S rDNA genes in barley by in situ hybridization. Genome 53: 1013-1018

18.
Levan, A., K. Frekga and A. Sandberg. 1964. Nomenclature for centromeric position in chromosomes. Hereditas 52: 201-220

19.
Maluszynska, J. and J.S. Heslop-Harrison. 1991. Localization of tandemly repeated DNA sequences in Arabidopsis thaliana. Plant J. 1: 159-166 crossref(new window)

20.
Matsuura, H. and T. Suto. 1935. Contributions to the ideogram study in Phanerogamous plants I. Jour. Fac. Sci. Hokkaido Imp. Univ. Ser. 5, Bot. 5: 33-75

21.
Park, S.H., S. R. Ohi, K.S. Ahn, J. G. Kim and H.K. Lee. 2002. Structure fetermination of a new lupen-type tetrapene, tiarellic acid, isolated from Tiarella polyphylla. Arch Pharm Res. 25: 57-60 crossref(new window)

22.
Schoennagel, E. 1931. Chromosomenzahl und Phylogenie der Saxifragaceen. Bot. Jahrb. 64: 266-308

23.
Skovsted, A. 1934. Cytological studies in the tribe Saxifrageae. Dansk Bot. Ark. 8: 1-52

24.
Soltis, D.E. and B.A. Bohm. 1984. Karyology and flavonoid chemistry of the disjunct species of Tiarella (Saxifragaceae). Syst. Bot. 9: 441- 447 crossref(new window)