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Development of Leaf Mutant Cultivars of Cymbidium goeringii by Ethyl-methane-sulfonate (EMS) Treatment.
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  • Journal title : Korean Journal of Plant Resources
  • Volume 24, Issue 1,  2011, pp.17-22
  • Publisher : The Plant Resources Society of Korea
  • DOI : 10.7732/kjpr.2011.24.1.017
 Title & Authors
Development of Leaf Mutant Cultivars of Cymbidium goeringii by Ethyl-methane-sulfonate (EMS) Treatment.
Shin, Yun-Ho; Song, In-Ja; Kang, Eun-Jung; Bae, Tae-Woong; Sun, Hyeon-Jin; Kang, Si-Young; Lim, Pyung-Ok; Lee, Hyo-Yeon;
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This study was for developing leaf chlorophyll mutant cultivars of Cymbidium goeringii by ethyl-methanesulfonate(EMS) treatment. Chlorophyll mutant rhizomes were easily produced by 0.2% EMS treatment in this genus. Among the mutants, they became dark brown about 50% of the rhizomes. When the dark-brown rhizomes were cultured in a solidified MS medium, new rhizomes were formed from part of the old ones. Chlorophyll mutant rhizomes were obtained from subcultured meristem tissues of newly-formed rhizomes. The rhizomes were cut and subcultured for a year and then became mutant plants. As the results, they produced 4 kinds of leaf mutant cultivars; zigzag-striped, comb-striped, net-striped, and dwarf types, indicating that the EMS treatment in the rhizome could produce versatile leaf chlorophyll regulating mutants. These results suggest that our method is useful for developing leaf mutant cultivars of this planta which they are estimated as higher commercial values.
EMS(ethyl-methane-sulfonate);Chlorophyll deficient;Mutant, Rhizome;
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