JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Anti-Inflammatory Effects of Ethyl Acetate Fraction from Cnidium officinale Makino on LPS-Stimulated RAW 264.7 and THP-1 Cells
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Korean Journal of Plant Resources
  • Volume 25, Issue 3,  2012, pp.299-307
  • Publisher : The Plant Resources Society of Korea
  • DOI : 10.7732/kjpr.2012.25.3.299
 Title & Authors
Anti-Inflammatory Effects of Ethyl Acetate Fraction from Cnidium officinale Makino on LPS-Stimulated RAW 264.7 and THP-1 Cells
Jeong, Jin-Boo; Hong, Se-Chul; Jeong, Hyung-Jin; Koo, Jin-Suk;
  PDF(new window)
 Abstract
This work aimed to elucidate the anti-inflammatory effects of ethyl acetate fraction from Cnidium officinale Makino with a cellular system of LPS-stimulated RAW 264.7 and THP-1 cells. Some key pro-inflammatory cytokines and mediators including NO, iNOS, , COX-2, TNF-, NF- p50 and NF- p65 were studied by sandwich ELISA and western blot analysis. Ethyl acetate fraction could significantly inhibit the production of NO, , TNF-, iNOS and COX-2 in LPS-stimulated cell than that of single LPS-stimulated. And ethyl acetate fraction suppresses the activation of NF- p50 and NF- p65. All the results showed that ethyl acetate fraction had a good anti-inflammatory effect on LPS-stimulated RAW264.7 and THP-1 cells. Taken together, the anti-inflammatory actions of ethyl acetate fraction from Cnidium officinale Makino might be due to the down-regulation of NO, , TNF-, iNOS and COX-2 via the suppression of NF- activation.
 Keywords
Cnidium officinale;NO;;TNF-;NF-;
 Language
English
 Cited by
1.
Lipopolysaccharide로 유도한 대식세포의 염증반응에서 미나리 에탄올 추출물 및 분획물의 항염증 효과,장지훈;조현우;이보영;유강열;윤지영;

한국식품영양과학회지, 2016. vol.45. 11, pp.1595-1603 crossref(new window)
2.
Murine Macrophage RAW 264.7 세포에서 구실잣밤나무 추출물의 항염증 효과,고영종;송상목;현우철;양수경;송창길;이동선;윤원종;

한국자원식물학회지, 2014. vol.27. 5, pp.439-446 crossref(new window)
3.
천궁 열수추출물 첨가가 고콜레스테롤 식이를 급여한 흰쥐의 지방대사에 미치는 영향,원향례;

한국지역사회생활과학회지, 2015. vol.26. 2, pp.351-360 crossref(new window)
4.
LPS로 유도된 RAW 264.7 cell로부터 NF-κB 조절 억제와 마우스 모델을 통한 큰잎모자반 에탄올 추출물의 항염증 효과,강보경;김꽃봉우리;김민지;박시우;박원민;안나경;최연욱;배난영;박지혜;안동현;

한국미생물생명공학회지, 2015. vol.43. 2, pp.112-119 crossref(new window)
5.
까마귀쪽나무 열매와 잎의 항염증 효과 비교 연구,남궁승;장선아;손은화;박종필;손은수;구현정;윤원종;권정은;정용준;맹학;한효상;강세찬;

한국자원식물학회지, 2015. vol.28. 2, pp.145-152 crossref(new window)
6.
Lipopolysaccharide로 유도된 RAW 264.7 세포에 대한 참치심장 물 추출물의 항염증 효과,김민지;배난영;김꽃봉우리;박지혜;박선희;조영제;안동현;

KSBB Journal, 2015. vol.30. 6, pp.326-331 crossref(new window)
7.
꽃지누아리 에탄올 추출물의 LPS로 유도된 RAW 264.7 세포에 대한 항염증 효과,김민지;배난영;김꽃봉우리;박지혜;박선희;최정수;안동현;

한국식품영양과학회지, 2016. vol.45. 2, pp.181-187 crossref(new window)
8.
구절초(Chrysanthemum zawadskii var. latilobum)에서 분리된 다당류의 대식세포 면역조절 활성,성낙윤;박우용;김이은;조은지;김미환;류기형;변의홍;박윤제;

한국식품영양학회지, 2016. vol.29. 3, pp.431-437 crossref(new window)
1.
Anti-inflammatory Effect of Castanopsis cuspidata Extracts in Murine Macrophage RAW 264.7 Cells, Korean Journal of Plant Resources, 2014, 27, 5, 439  crossref(new windwow)
2.
Comparative Study of Litsea japonica Leaf and Fruit Extract on the Anti-inflammatory Effects, Korean Journal of Plant Resources, 2015, 28, 2, 145  crossref(new windwow)
3.
Immuno-Modulatory Activities of Polysaccharides separated from Chrysanthemum zawadskii var. latilobum in Macrophage Cells, The Korean Journal of Food And Nutrition, 2016, 29, 3, 431  crossref(new windwow)
4.
Anti-Inflammatory Effect of Grateloupia imbricata Holmes Ethanol Extract on LPS-Induced RAW 264.7 Cells, Journal of the Korean Society of Food Science and Nutrition, 2016, 45, 2, 181  crossref(new windwow)
5.
Effects of the Cnidium Officinale Makino Water Extract on the Lipid Metabolism of Rats Fed a Hypercholesterol Diet, The Korean Journal of Community Living Science, 2015, 26, 2, 351  crossref(new windwow)
6.
Anti-inflammatory Effect of Water Extract from Tuna Heart on Lipopolysaccharide-induced Inflammatory Responses in RAW 264.7 Cells, KSBB Journal, 2015, 30, 6, 326  crossref(new windwow)
 References
1.
Ahn, M.Y., K.S. Ryu, Y.W. Lee and Y.S. Kim. 2000. Cytotoxicity and L-amino acid oxidase activity of crude insect drugs. Arch. Pharm. Res. 23:477-481. crossref(new window)

2.
Andreakos, E., B. Foxwel and M. Feldmann. 2002. Is targeting Toll-like receptors and their signaling pathway a useful therapeutic approach to modulating cytokine-driven inflammation? Immunol. Rev. 202:250-265.

3.
Banskota, A.H., Y. Tezuka, N.Y. Nguyen, S. Awale, T. Vobukawa and S. Kadota. 2003. DPPH radical scavenging and nitric oxide inhibitory activities of the constituents from the wood of Taxus yunnanensis. Planta Med. 69:500-505. crossref(new window)

4.
Bosca, L., M. Zeini, P.G. Traves and S. Hortelano. 2005. Nitric oxide and cell viability in inflammatory cells: a roll for NO in macrophage function and fate. Toxicology 208:249-258. crossref(new window)

5.
Buttery, L.D., T.J. Evans, D.R. Springall, A. Carpenter, J. Cohen and J.M. Polak. 1994. Immunochemical localization of inducible nitric oxide synthase in endotoxin-treated rats. Lab. Invest. 71:755-764.

6.
Jeong, S.I., D.H. Kwak, S. Lee, Y.K. Choo, W.H. Woo, K.S. Keum, B.K. Choi and K.Y. Jung. 2005. Inhibitory effects of Cnidium offcinale Makino and Tabanus fulvus Meigan on the high glucose-induced proliferation of glomerular mesangial cells. Phytomedicine 12:648-655. crossref(new window)

7.
Jung, H.W., U.K. Seo, J.H. Kim, K.L. Leem and Y.K. Park. 2009. Flower extracts of Panax notoginseng attenuates lipopolysaccharide- induced inflammatory response via blocking of NF-kB signaling in murine macrophages. J. Ethnopharmacol. 122:313-319. crossref(new window)

8.
Libby, P., P.M. Ridker and A. Maseri. 2002. Inflammation and atherosclerosis. Circulation 105: 1135-1143. crossref(new window)

9.
Lim, J.H., Y.M. Park, J.S. Kim, H.J. Jeong and E.W. Seo. 2011. Effect of Cnidium officinale extract on recovery capability of allergic contact-dermatitis in Rat. Korean J. Plant Res. 24:430-437 (in Korean). crossref(new window)

10.
Locksley, R.M., N. Killeen and M.J. Lenardo. 2001. "The TNF and TNF receptor superfamilies: integrating mammalian biology". Cell 104:487-501. crossref(new window)

11.
Menichini, F., F. Conforti, D. Rigano, C. Formisano, F. Piozzi and F. Senatore. 2009. Phytochemical compositon, anti-inflammatory and antitumour activities of four Teucrium essential oils from Greece. Food Chem. 115:679-686. crossref(new window)

12.
Moncada, S. 1999. Nitric oxide: discovery and impact on clinical medicine. Journal of the Royal Society of Medicine 92:164- 169.

13.
Nathan, C. 2002. Nitric oxide as a secretary product of mammalian cells. FASEB J. 6: 3051-3064.

14.
Pokharel, Y.R., Q.H. Liu, J.W. Oh, R. Woo and K.W. Kang. 2007. 4-Hydroxykobusin inhibits the induction of nitric oxide synthase by inhibiting NF-kB and AP-1 Activation. Biol. Pharm. Bull. 30:1097-1101. crossref(new window)

15.
Rishton, G.M. 2008. Natural products as a robust source of new drugs and drug leads: past successes and present day issues. Am. J. Cardiol. 101:43D-49D.

16.
Rocca, B. and G.A. FitzGerald. 2002. Cyclooxygenases and prostaglandins: shaping up the immune response. Int. Immunopharmacol. 2:603-630. crossref(new window)

17.
Sim, J.S., J.B. Jeong, J.H. Lee, T.H. Kwon, Y.J. Cha and H.J. Jeong. 2010. Inhibitory effect of the phenolic compounds from apples against oxidative damage and inflammation. Korean J. Plant Res. 23:487-497.

18.
Turini, M.E. and R.N. DuBois. 2002. Cyclooxygenase-2: a therapeutic target. Annu. Rev. Med. 53:35-57. crossref(new window)

19.
Yang, C.W., W.L. Chen, P.L. Wu, H.Y. Tseng and S.J. Lee. 2006. Anti-Inflammatory mechanisms of phenanthroindolizidine alkaloids. Mol. Pharmacol. 69:749-758.

20.
Yin, H., F. Zhang, M. Yu, H. Cheng, J. Lin, Y. Gao, B. Han and L. Zhu. 2005. Beta-endorphin ameliorates synovial cell hyperfunction in the collagen-induced arthritis rat model by specific downregulation of NF-kappa B activity. Neuroendocrinology 81:10-18. crossref(new window)

21.
Yoon, W.J., Y.M. Ham, B.S. Yoo, J.Y. Moon, J.S. Koh and C.G. Hyun. 2009. Oenothera laciniata inhibits lipopolysaccharide induced production of nitric oxide, prostaglandin $E_{2}$, and proinflammatory cytokines in RAW264.7 macrophages. J. Biosci. Bioeng. 107:429-438. crossref(new window)

22.
Yoshimura, A. 2006. Signal transduction of inflammatory cytokines and tumor development. Cancer Sci. 97:439-447. crossref(new window)

23.
Zhao, F., L. Wang and K. Liu. 2009. In vitro anti-inflammatory effects of arctigenin, a lignan from Arctium lappa L., through inhibition on iNOS pathway. J. Ethnopharmacol. 122:457-462 crossref(new window)