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Anti-Inflammatory Effects of Ethyl Acetate Fraction from Cnidium officinale Makino on LPS-Stimulated RAW 264.7 and THP-1 Cells
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  • Journal title : Korean Journal of Plant Resources
  • Volume 25, Issue 3,  2012, pp.299-307
  • Publisher : The Plant Resources Society of Korea
  • DOI : 10.7732/kjpr.2012.25.3.299
 Title & Authors
Anti-Inflammatory Effects of Ethyl Acetate Fraction from Cnidium officinale Makino on LPS-Stimulated RAW 264.7 and THP-1 Cells
Jeong, Jin-Boo; Hong, Se-Chul; Jeong, Hyung-Jin; Koo, Jin-Suk;
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This work aimed to elucidate the anti-inflammatory effects of ethyl acetate fraction from Cnidium officinale Makino with a cellular system of LPS-stimulated RAW 264.7 and THP-1 cells. Some key pro-inflammatory cytokines and mediators including NO, iNOS, , COX-2, TNF-, NF- p50 and NF- p65 were studied by sandwich ELISA and western blot analysis. Ethyl acetate fraction could significantly inhibit the production of NO, , TNF-, iNOS and COX-2 in LPS-stimulated cell than that of single LPS-stimulated. And ethyl acetate fraction suppresses the activation of NF- p50 and NF- p65. All the results showed that ethyl acetate fraction had a good anti-inflammatory effect on LPS-stimulated RAW264.7 and THP-1 cells. Taken together, the anti-inflammatory actions of ethyl acetate fraction from Cnidium officinale Makino might be due to the down-regulation of NO, , TNF-, iNOS and COX-2 via the suppression of NF- activation.
Cnidium officinale;NO;;TNF-;NF-;
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