Shoot Development thorough Cytokinin Treatments in Ovary Culture of Three Interspecific Hybrid Lilies Kim, Seung Deok; Kim, Ju Hyung; Lee, Jong Won; Lee, Ki Yeol; Kim, Tae Jung; Paek, Kee Yoeup;
We performed the following experiment to increase the development of shoots in the in vitro culture of hybrid progeny. MS medium was used as the base medium; cytokinins BA at a concentration of 2.0, 3.0, or 5.0 ㎎/L and TDZ at a concentration of 0.2, 0.3, or 0.5 ㎎/L were added to the in vitro cultures. For the FO progeny, the shoot development rate was highest of 18% with 2.0 ㎎/L BA, at 18%, and 3.0 ㎎/L and 5.0 ㎎/L BA resulted in a lower rate of 12%. TDZ addition tended to identical or similar results to that of the control media. For the FA progeny, addition of TDZ tended to lead to a higher shoot development rate and was superior with 0.3 ㎎/L treatment. However, the addition of BA to the media resulted in a shoot development rate between 14.3% and 15.7%, similar to that of the control.
Asano, Y. 1981. Pollen-tube growth in interspecific crosses on Lilium longiflorum (Ii). J. japan. Soc. Hort. Sci. 50:350-354.
Ascher, P.D. and S.J. Peloquin. 1968. Pollen tube growth and incompatibility following intra- and inter-specific pollinations in Lilium longiflorum. Amer. J. Bot. 55:1230-1234.
Chichiricco, G. 1996. Intra- and interspecific reproductive barriers in Crocus (Iridaceae). Plant Systematics and Evolution 201:83-92.
Ghosh, S. and K.R. Shivanna, 1980. Pollen pistil interaction in Lilium grandiflorum scanning electron microscopic observations and proteins of the stigma surface. Planta 149:257-261.
Lee, H.K. 2002. Improvement of breeding efficiency in interspecific hybridization of lilies. Ph.D Thesis, Seoul National Univ., Korea.
Loretta B., C.R. Patrizio, B. Claudia and S. Francesco. 2002. Adventitious shoot regeneration from leaf explants and stem nodes of Lilium. Plant Cell, Tissue and Organ Culture 74:37-44.
North, C. and A.B. Wills. 1969. Inter-specific hybrid of Lilium, lankongense Franchet produced by embryo-culture. Euphytica 18:430-434.
Kim, E.Y., J.D. Choi, K.I. Park, M.S. Byun and K.W. Kim. 1998. Induction of multiple shoots through microscale section culture in Lilium oriental hybrid. Korean J. Hort. Sci. 16:410-410.
Kim, E.Y., J.D. Choi, K.I. Park, M.S. Byun and K.W. Kim. 1999. Enhancement of proliferation rate through multiple shoot induction from culture of microscale section in Lilium. Korean J. Hort. Sci. 40:459-462.
Knox. 1986. An extensive review of technologies used to restore contaminated aquifers. p. 764.
Palacios, R., E. Martinez, M. Flores and D. Romero. 1990. Organization and dynamics of the Rhizobium genome. A Basis for Introducing Novel Arrangements of Genetic Information into the Environment. Scientific Committee on Problems of the Environment International Council of Scientific Unions. 44:69-78.
Park, H.B. and E.G. Choi. 1994. Effect of thidiazuron on bulb scale and floral organ culture of Lilium longiflorum. Chonbuk National Univesity. Institute of Agricultural Science & Technology Korea. 38:205-212.
Van Tuyl, J.M. 1988. Effect of temperature treatments on the scale propagation of Lilium longiflorum ‘White Europe’ and Lilium × ‘Enchantment’. Hort. Sci. 18:754-756.
Yoon, E.S. and S.R. Lee. 1988. Study of karyotype, meiosis and isozyme of hybrid from cross Lilium longiflorum × L. × elegans. Korean J. Plant Res. 1(1):80-87.
Yoon, E.S. 1990. Growth of pollen tube between self pollination and interspecific pollination of Lilium genus. Korean J. Plant Res. 3(2):123-128.